GE ÄKTA start Maintenance Manual
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GE ÄKTA start Maintenance Manual

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ÄKTA ™ start
Maintenance Cue Card
Overview
ÄKTA start is a simple and modern liquid chromatography system intended for
preparative purification of proteins at laboratory scale. The system can be used for
a variety of research purposes to fulfill the needs of the users in the academia and in
the life sciences industry.
The Maintenance Cue Card describes the maintenance schedule for ÄKTA start and
Frac30 (the Fraction collector), and provides instructions for routine maintenance.
Regular maintenance is important for safe and trouble-free operation of ÄKTA start.
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Summary of Contents for GE ÄKTA start

  • Page 1: Table Of Contents

    ÄKTA ™ start Maintenance Cue Card Contents System overview 2 Flow path overview 4 Regular maintenance 4 Cleaning system flow path 5 Clean the column 7 Other maintenance procedures 7 Storage of the instrument 11 Safety precautions 11 Getting help 12 Reference information 12 Overview ÄKTA start is a simple and modern liquid chromatography system intended for...

  • Page 2: System Overview

    System overview Create method: create, edit, import and delete methods Part Description Settings and service: configure, calibrate, troubleshoot ÄKTA start and diagnose modules Frac30, Fraction collector Instrument illustration PC with UNICORN™ start software The illustration below shows the locations of the modules placed on the wet side of the instrument.
  • Page 3 • Evaluating and analyzing the results from ÄKTA start Frac30 , Fraction collector Module Features System Provides an intuitive and easy-to-use interface to control ÄKTA start. Control Part Description Part Description Performs and monitors manual Dispenser arm Bowl assembly chromatography runs. Tubing holder Base unit Performs and monitors automated...

  • Page 4: Flow Path Overview

    Flow path overview The illustration below shows the flow path for ÄKTA start. Part Description Part Description Part Description Buffer valve Pressure sensor UV Monitor Mixer Wash valve Conductivity Monitor Sample valve Injection valve (manual) Outlet valve Pump Column Fraction collector Regular maintenance Interval Maintenance action...

  • Page 5: Cleaning System Flow Path

    Interval Maintenance action ÄKTA start When Clean the Fraction collector. required Method run Note: Frac30 is not a spill-free design. There will be some spillage when tubes shift position, depending on the flow rate. Prepare system Perform System cleaning, Pump Wash A and B, Washout Fractionation tubing.
  • Page 6 Operation from the Instrument Display Instruction Perform the following operations to run Washout Clean the inlets and outlets (buffer tubing, sample inlet fractionation tubing. tubing, fractionation tubing) Immerse the buffer inlet tubing (A & B) in 1 M NaOH. ÄKTA start Disconnect column from the flow path and reconnect the tubing with a Union connector.

  • Page 7: Clean The Column

    Clean the column * Luer connectors are a type of fittings used for making leak-free connections by interlocking the counter-parts (male and female connectors). Cleaning Clean the Conductivity flow cell After a run is completed, clean the column if required. Maintenance interval Instruction Clean the Conductivity flow cell when the Conductivity...
  • Page 8 Instruction Instruction Disconnect the inlet tubing from the Pressure sensor Immerse the buffer inlet tubing in DM water. to expose the sensor to atmospheric pressure only. Flush the UV flow cell with DM water using Pump. Make Access Settings and service > Pressure sensor. sure that there are no air bubbles in the UV flow cell.
  • Page 9 • Make sure to take note of all the required observations Note the current temperature of the calibration by recording parameters while the performance run solution in the conductivity flow cell as displayed in the is in progress. Enter the observed values in the ÄKTA Cell temperature field.
  • Page 10 Operation from UNICORN start Replace the inlet filters Instruction Note: An Inlet Filter Set containing inlet filters and support nets is included in the accessories kit. Select method based on the Fraction Collector configuration: a) Performance method with Frac: when Instruction Fraction collector is enabled;...

  • Page 11: Storage Of The Instrument

    These user instructions do not guarantee user safety - it is the responsibility of the user’s employer to ensure safe operation and training. Training courses are available and can be arranged by contacting your local GE Healthcare office. ÄKTA start Maintenance Cue Card, 29-0240-43 AC...

  • Page 12: Getting Help

    Health and Safety Declaration Form All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them. A Please complete the checklist in the On Site Service copy of these terms and conditions is available on request. Contact Health &...

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