Procedure For Microscopy Under Episcopic Illumination - Nikon SMZ25 Instruction Manual

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Procedure for Microscopy under Episcopic Illumination

This section describes the procedure for performing microscopy under episcopic illumination using the SMZ18 Zooming
Body combined with the P2-CIA Coaxial Epi Illuminator.
1
Turn on the power.
Turn on the P2-CTLB Control Box by pressing the power
switch to the "|" position.
Check that the power LED on the control box lights.
Power supply to the DS-L4 camera control
unit
When the DS-L4 camera control unit is connected,
turn on the control box and then the DS-L4.
2
Set a sample.
Place a sample on the stage plate and immediately under
the objective.
3
Bring an objective into the optical path.
Turn the nosepiece to bring the objective to be used into
the optical path.
Check that the objective falls into the click position when
turning the nosepiece.
(See Chapter 7, "8.2 Switching Objectives.")
P2-CIA Coaxial Epi Illuminator
• The P2-CIA Coaxial Epi Illuminator is supplied
with a 1/4 lambda plate (optional). When using
the 0.5x or 1x objective, attach the 1/4 lambda
plate to the tip of the objective. Turn the 1/4
lambda plate so that the white circle (indicator) is
at the front.
• For the P2-CIA Coaxial Epi Illuminator, available
objectives and zooming ratios are restricted. For
details, see Chapter 8, "7.1 P2-CIA Coaxial Epi
Illuminator."
Chapter 2
Microscopy Procedures
9
Power on
Sample
Setting the sample
Rotating the nosepiece
Power LED: On
Power switch: "|"
position
Objective on
the optical path