Using Diascopic Illumination - Nikon SMZ25 Instruction Manual

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Using Diascopic Illumination

The microscope system enables diascopic illumination when
used with a diascopic illumination base or a diascopic illumination
stand. All of the devices use LEDs as the light source and provide
uniform illumination.
The image to be observed results from diascopic illumination
which passes through the sample and enters the objective.
Diascopic illumination is used for observing transparent
substances such as macromolecular compounds, fibers, and
glass and tissue sections and biological specimens in petri
dishes.
In addition, a P-DF2 LED Dark Field Unit 2 or a P2-POL Simple
Polarizing Attachment can be attached to enable dark-field
diascopic illumination and diascopic simplified/polarized
illumination. This enables observation of samples that are difficult
to be observed under ordinary bright-field diascopic illumination
such as transparent materials and crystals and tissues and
minute organisms in culture solutions.
See Chapter 4 "8 Diascopic Illuminator" and select the
appropriate device based on the objective of microscopy.
• P2-DBL LED Diascopic Illumination Base
• P2-DBF Fiber Diascopic Illumination Base
• P-DSL32 LED Diascopic Illumination Stand
• P-DSF32 Fiber Diascopic Illumination Stand
• P-DF2 LED Dark Field Unit 2
• P2-POL Simple Polarizing Attachment
Empty cube
When an epi-fluorescence attachment is mounted, check that the empty cube of the attachment is in the optical path.
OCC illumination
The P2-DBL LED Diascopic Illumination Base, the P2-DBF Fiber Diascopic Illumination Base, the P-DSL32 LED
Diascopic Illumination Stand and the P-DSF32 Fiber Diascopic Illumination Stand support OCC illumination (oblique
illumination). OCC illumination, which offers high contrast, is appropriate for microscopy of phase objects.
Chapter 7
Specific Operations
80
Diascopic illumination with the P2-DBL LED
Diascopic Illumination Base