Nikon SMZ25 Instruction Manual page 133

Bright-field episcopic observation
This is useful for observing the entire image of an opaque specimen during fluorescence observation. Although the
P2-EFLBFA Filter Cube (Bright Field) contains a UV blocking filter, adjust the brightness of the illuminator so that
illumination is not too bright. A 1/4 λ plate is supplied as an option for the P2-EFLBFA to prevent flare in the imaging
system due to light reflected into the optical system. Attach the 1/4 λ plate to a 0.5x or 1x objective.
When observing light reflected from a specimen with a mirror-like surface, the brightness on the right optical path differs
from that on the left optical path. Also, duplicated images may be produced by the internal half mirror.
OCC light shielding plate
When using an epi-fluorescence attachment with a diascopic illumination base, Nikon recommends closing the OCC light
shielding plate on the dia-illumination completely. This can decrease background noise caused by intrinsic fluorescence in
the diascopic illuminator.
 1/4 λ plate
When using a P2-EFLBFA Filter Cube (Bright Field) with a
0.5x or 1x objective, an optional 1/4 λ plate must be attached
to the tip of the objective.
1/4 λ plates are supplied to prevent flare in the imaging
system due to light reflected into the optical system.
There are the following two types of 1/4 λ plates.
• P2-CIA QL0.5X 1/4λplate 0.5x for coaxial epi illuminator:
Intended for 0.5x objectives
• P2-CIA QL1X 1/4λplate 1x for coaxial epi illuminator:
Intended for 1x objectives
When using a 0.5x objective, remove the protection ring from
the tip of the objective, and then screw in the 1/4 λ plate.
When using a 1x objective, remove the correction glass from
the tip of the objective, and then screw in the 1/4 λ plate.
The brightest illumination is generated when the white dot on
the 1/4 λ plate is at the front.
Select a position that has the optimum brightness and contrast
for observation.
For details about using the light source, refer to the instruction manual for the light source.
Chapter 8
Functions and Operations of the Devices
119
White dot
1/4 λ plate