Using Epi-Fluorescence Illumination; Switching Filter Cubes - Nikon SMZ25 Instruction Manual

13

Using Epi-Fluorescence Illumination

Epi-fluorescence microscopy used for observing fluorescence
images requires an optical element such as a fluorescence filter,
and a brighter light source including mercury lamp.
In this microscope system, state detection or motorized-type
epi-fluorescence illumination is available. With a filter cube
attached to the device, the filter cube is brought into the optical
path by the rotation of the turret, resulting in the generation of
epi-fluorescence illumination. The light source is a precentered
high-intensity mercury lamp.
Illumination led from the light source to the device by the HG fiber
passes through a fluorescence filter and emits excitation light to
the sample so that fluorescent images can be observed.
See Chapter 8, "9 Epi-Fluorescence Attachment" and select the
appropriate device based on the objective of microscopy.
• P2-EFLI Epi Fluorescence Attachment
• P2-EFLM Motorized Epi Fluorescence Attachment
For details on adjusting the light sources such as brightness
adjustment, refer to the instruction manuals of the light sources.
WARNING
The light source used with the epi fluorescence attachment (HG precentered fiber illuminator) requires special care during
handling because of its characteristics. Make sure you are familiar with and observe all warnings and cautions described at the
beginning of this instruction manual.

Switching Filter Cubes

13.1
The turret of the epi fluorescence attachment is rotated to switch the four filter cubes attached to the turret.
[Turret rotation operation]
P2-EFLI Epi Fluorescence Attachment:
P2-EFLM Motorized Epi Fluorescence Attachment: Filter cube switching buttons of the remote controller
BIN O
PHO TO
On the P2-EFLI Epi Fluorescence Attachment, turn the filter cube switching knob to assign to the indicator the turret address
(FL1 to FL4) of the filter cube to be placed into the optical path.
On the remote control, press the filter cube switching buttons (FL1 to FL4) to specify the turret address of the filter cube to be
placed into the optical path. The LED of the pressed button lights so that the turret address on the optical path can be
identified. The motorized epi fluorescence attachment has a turret address view window on the right front so that the turret
address on the optical path can be identified.
Chapter 7
Specific Operations
Filter cube switching knob of the device
Filter cube switching
knob
Switching filter cubes
81
Epi-fluorescence illumination with the epi
fluorescence attachment
Filter cube switching
buttons
FL Cube FL Cube
1 2 3 4
DISPLAY DISPLAY Z-RESET Z-RESET
+
Z-SPEED Z-SPEED ZOOM STEP ZOOM STEP
Coarse Coarse Fine Fine Ex Fine Ex Fine 1x 1x 0.5x 0.5x 0.1x 0.1x
−
ZOOM ZOOM
ESCAPE ESCAPE LIMIT LIMIT
STEP STEP
ON/OFF ON/OFF ON/OFF ON/OFF
− +
ALZ ALZ
ON/OFF ON/OFF ON/OFF ON/OFF