Nikon SMZ25 Instruction Manual page 127

 Power supply and illumination control
Power to the P-DSL32 LED Diascopic Illumination Stand is supplied by connecting a P2-CTLB Control Box or a 12 V AC
adapter. To provide diascopic illumination without using a control box, connect a 12 V AC adapter.
Turn on or off the LED diascopic illuminator using the LED power ON/OFF switch at the front of the base.
Adjust illumination using the LED brightness control dial on the base. To enable adjustment using the LED brightness control
dial on the base, the LED brightness control selection switch must be turned off.
[Controlling illumination from the P2-CTLB Control Box]
When the P2-CTLB Control Box is connected to the base, turn on the LED brightness control selection switch. In this state,
diascopic illumination can be turned on or off, brightness can be controlled, and light intensity can be monitored from the
control box.
Actual control is carried out by the PC (software: NIS-Elements) connected to the control box.
Note that illumination cannot be controlled from the control box, even if the LED brightness control selection switch is turned
on, unless the LED power ON/OFF switch on the base is turned on. Be sure to turn on the LED power ON/OFF switch.
• LED brightness control selection switch on:
• LED brightness control selection switch off:
For details, see "11.1 P2-CTLB Control Box."
 OCC (oblique coherent contrast) illumination
This stand supports OCC illumination so that a transparent sample can be observed at high contrast using diascopic
illumination with a white LED. OCC illumination is an oblique illumination method suitable for observing the phase objects,
where a sample is obliquely illuminated with a coherent illuminating light by shielding a part of the optical path using a
shading plate. With this illumination method, a colorless transparent sample can be observed with relief and contrast.
To adjust the OCC contrast, slide the OCC adjustment knob at the right front of the base back and forth. Adjust until the
optimum observation state is obtained in combination with the selected magnification and phase amount of the sample.
For ordinary bright-field microscopy under diascopic illumination, set the OCC adjustment control to the either the farthest
or closest position. Sliding the control gradually forward from that position will cause the OCC effect to increase. Sliding
the control by 46 mm will result in a completely shielded state.
In addition, the control position (knob position) can be reproduced using the OCC adjustment knob with a scale.
Objective
0.5X
1X
1.6X
2X
* Shading may occur in the low zoom magnification range.
Increasing contrast of the OCC observation image
For observations of the OCC observation image with higher contrast, remove the filter dropping ring (attached to the base
optical path immediately under the glass plate). The contrast can be increased by removing the filter dropping ring that
contains a diffuser plate to control excess contrast for OCC.
 Focusing mechanism
The support moves vertically using the focus dial at the front of the base so that samples with height variations can easily
be in focus.
• Focusing stroke: 6.2 mm
• Stroke per focus dial rotation: 3.24 mm
Chapter 8
Functions and Operations of the Devices
Assigns the control right to the control box.
Assigns the control right to the base.
Usable objectives and recommended zoom magnifications
Bright-field diascopic illumination
Entire area
Zoom magnification
From 1.3x
From 5.0x
From 7.0x
113
OCC illumination
Contrast in the field of view is not uniform.
From 5.0x
From 5.0x
From 7.0x