Using Diascopic Illumination - Nikon SMZ800N Instruction Manual

12

Using Diascopic Illumination

The microscope system enables diascopic illumination when
used with the diascopic illumination stand. Diascopic illumination
stands that use LEDs as the light source provide bright and
uniform illumination.
Observe an image visible by diascopic illumination that passes
through the sample and enters the objective. Diascopic
illumination is used for observing transparent substances such as
macromolecular compounds, fibers, and glass and tissue
sections and biological specimens in petri dishes.
In addition, a P-DF2 LED Dark Field Unit 2 or a C-POL Simple
Polarizing Attachment can be attached to enable dark-field
diascopic illumination and diascopic simplified/polarized
illumination. This enables observation of samples that are difficult
to observe using ordinary bright-field diascopic illumination such
as transparent materials and crystals and tissues and minute
organisms in culture solutions.
When performing microscopy using diascopic illumination with a
C-FMCN Focus Mount attached, match the observation axis with
the optical axis of the diascopic illumination stand as follows:
(1) Set the zoom magnification to the smallest.
(2) Loosen the fixing knob of the C-FMCN Focus Mount.
(3) Move the C-FMCN Focus Mount to align the observation
axis with the optical axis of the diascopic illumination stand.
Be careful not to make the periphery dark.
(4) Tighten the fixing knob of the C-FMCN Focus Mount.
Loosen the fixing knob of the C-FMCN Focus Mount
before aligning the optical axes.
Do not move the C-FMCN Focus Mount before loosening the fixing
knob of the C-FMCN Focus Mount. Doing so may cause failure or
malfunction.
See Chapter 4, "12 Diascopic Illuminators" and select the
appropriate device based on the objective of microscopy.
 C-DS Dia Illumination Stand
 C-LEDS Hybrid LED Stand
 P-DSL32 LED Diascopic Illumination Stand
 P-DSF32 Fiber Diascopic Illumination Stand
 P-DF2 LED Dark Field Unit 2
 C-POL Simple Polarizing Attachment
Empty cube
When an epi-fluorescence attachment is mounted, check that the empty cube of the attachment is in the optical path.
OCC illumination
The P-DSL32 LED Diascopic Illumination Stand and the P-DSF32 Fiber Diascopic Illumination Stand support OCC
illumination (oblique illumination). OCC illumination, which offers high contrast, is appropriate for microscopy of phase
objects.
Chapter 3
Specific Operations
22
Diascopic illumination with the P-DSL32 LED
Diascopic Illumination Stand