Nikon SMZ25 Instruction Manual page 125

 OCC (oblique coherent contrast) illumination
This device supports OCC illumination so that a transparent sample can be observed at high contrast using diascopic
illumination with a white LED. OCC illumination is an oblique illumination method suitable for observing the phase objects,
where a sample is obliquely illuminated with a coherent illuminating light by shielding a part of the optical path using a
shading plate. With this illumination method, a colorless, transparent sample can be observed with relief and contrast.
To adjust the OCC contrast, turn the OCC adjustment dial. Adjust until the optimum observation state is obtained in
combination with the selected magnification and phase amount of the sample. In addition, the control position (dial
position) can be reproduced using the adjustment knob with a scale.
 Condenser lens
The base contains condenser lenses for low/high-magnification microscopy. Switch the condenser lens based on the
objective being used to ensure uniform diascopic illumination. A wide range of observation magnifications are supported.
To switch the condenser lens, slide the condenser lens switching lever to the right or left.
Slide the lens switching lever to the right: For low magnification; objective lens 0.5x to 1x
Slide the lens switching lever to the left:
When the condenser lens is switched, the built-in prism sheet is automatically switched and the shading adjusted.
P2-SHR Plan Apo 0.5X and condenser lens for low magnification
P2-SHR Plan Apo 01X and condenser lens for low magnification
P2-SHR Plan Apo 1.6X and condenser lens for high magnification
P2-SHR Plan Apo 2X and condenser lens for high magnification
* Shading may occur in the low zoom magnification range.
 Filter
The base has three different filters: NCB11, ND4, and ND16. The individual filters can be inserted into and removed from
the optical path separately. To insert a filter into the optical path, push in the filter IN/OUT lever.
 Attachable optional devices
The following devices can be attached to this base:
• P-SXY64 XY Stage
•
C-SSL DIA Sliding Stage
•
P-DF2 LED Dark Field Unit 2
•
C-TRS Tilting Stage
•
C-LSL2 LED Epi Illuminator 2 (via an SMZ-U Epi Arm)
• C-FDF Flexible Double Arm Fiber Illumination Unit (via a C-FIDH Fiber Holder)
•
P2-POL Simple Polarizing Attachment
45-mm dia. filter
A 45-mm dia. filter (no thicker than 2 mm) can be dropped onto the filter dropping ring (attached to the base optical path
immediately under the grass plate).
Chapter 8
Functions and Operations of the Devices
For high magnification; objective lens 1.6x to 2x
Usable objectives and recommended zoom magnifications
Objective
Zoom magnification
Bright-field diascopic
illumination
From 1.5x
Entire area
Entire area
From 2.0x
111
OCC illumination
From 4.5x
From 4.0x
From 4.0x
From 6.0x