Nikon SMZ25 Instruction Manual
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Nikon SMZ25 Instruction Manual

Research stereo microscope
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M608 E
13.5.NF.1
*M608EN01*
Research Stereo Microscope
SMZ25/SMZ18
Instruction Manual

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  • Page 1 M608 E 13.5.NF.1 *M608EN01* Research Stereo Microscope SMZ25/SMZ18 Instruction Manual...

  • Page 3: Introduction

    Zooming Body. To ensure correct usage, read this manual carefully before operating this product. ● No part of this manual may be reproduced or transmitted in any form without prior written permission from Nikon. ● The contents of this manual are subject to change without notice.

  • Page 4: Contents Of The Manual

    Introduction Contents of the Manual Chapter 1, “Components” in this instruction manual is presented, based on the system configuration examples of SMZ25 Zooming Body and SMZ18 Zooming Body. Chapter 2, “Microscopy Procedures” describes three different microscopy procedures in accordance with the configuration examples in Chapter 1. Chapter 3, “Operations for Each Purpose”...

  • Page 5: Table Of Contents

    Contents Contents Introduction ......................... i Contents of the Manual ....................ii Symbols Used in This Manual ..................ii Safety Precautions ......................vi WARNING and CAUTION Symbols ................vi Label Attached to the Product ..................vi WARNING ........................ viii CAUTION ........................x Notes on Handling the Product ..................
  • Page 6 Internally Saved Information ................... 36 Functions and Operations of the Devices ........... 37 Zooming Body ......................38 SMZ18 Zooming Body ..................38 SMZ25 Zooming Body ..................39 Focus Units......................41 P2-FU Focus Unit ..................... 41 P2-MFU Motorized Focus Unit ................. 42 P2-FMDN Focus Mount ..................
  • Page 7 Contents Assembling .................... 71 System Configuration ..................... 72 Device List ......................74 Assembly Procedures .................... 75 Assembling the EPI System and DIA + EPI-FL System ........75 Compact System Assembly ................91 Assembly of Other Devices (Optional) ............. 94 Troubleshooting ..................97 Image Viewing ......................

  • Page 8: Safety Precautions

    Safety Precautions Safety Precautions To ensure correct and safe operation, read this manual before using this product. WARNING and CAUTION Symbols Although this product is designed and manufactured to be completely safe during use, incorrect usage or failure to follow the safety instructions provided may cause personal injury or property damage.
  • Page 9 Safety Precautions Heat warning label Caution: Heat of the empty cube Immediately after diascopic observation using an epi-fluorescence attachment, the front face of the empty cube may become hot, resulting in burns. Do not touch the front face of the empty cube.
  • Page 10 (which may take observed. approximately 30 minutes) before attempting to Safety is a top design priority for Nikon products. replace the lamp. Safety is ensured as long as the user observes all ● Do not place fabric, paper, or highly flammable of the warnings and cautions given in the manuals, volatile materials (i.e.
  • Page 11 Safety Precautions WARNING Hazards of mercury lamps Hazardous sample handling (when using the epi-fluorescence attachment) This product can also be used for microscopy and The light source used with the epi-fluorescence imaging of biological samples (cells/tissues) in a attachment (HG Precentered Fiber Illuminator) petri dish.
  • Page 12 Do not stare at operating lamp. product, stop using the product, and contact your May be harmful to the eyes. nearest Nikon representative. RISK GROUP 2 IEC62471: 2006” Remove any covers from the product before Be careful of the heat of the empty cube switching on.
  • Page 13 Safety Precautions CAUTION ● The total weight of the microscope system is over 20 kg. When moving the system to another location, separate the removable devices temporarily and carry the system while firmly holding the hand holding portions of the base, with multiple persons.

  • Page 14: Notes On Handling The Product

    Notes on Handling the Product Notes on Handling the Product Handle the product carefully. Handling a focus knob ● Never turn the focus knobs in opposite directions This product is a precision instrument. Avoid at the same time. Doing so may damage the subjecting it to sudden impacts and shocks.

  • Page 15: Components

    Chapter Components In the stereo microscopes SMZ25 and SMZ18, the SMZ25 Zooming Body or SMZ18 Zooming Body is attached to a motorized or manual focus mount. In addition, arbitrary models can be selected and combined from such device models as bases, objectives, tubes and episcopic illuminators.

  • Page 16: Microscopy System Under Episcopic Illumination

    Chapter 1 Components Microscopy System under Episcopic Illumination Names of the Devices and Operation Sections DS camera head P2-TERG 100 Optical path switching lever Trinocular Tilting Tube P2-FU Focus Unit P2-RLY Relay Box P2-CI Coaxial Epi Illuminator (To P2-CTLB Control Box) Zooming knob P2-RNI2 Intelligent Nosepiece...

  • Page 17: Microscopy System Under Diascopic Illumination And Epi-Fluorescence Illumination

    P2-RNI2 Intelligent Nosepiece P2-EFLM Motorized Epi Fluorescence P2-RC Remote Controller Attachment (See “2.2 Buttons on the P2-RC Remote Controller.”) SMZ25 Zooming Body P2-CTLA Control Box C-HGFIE HG precentered fiber Light shielding plate illuminator (See the instruction manual for the HG...

  • Page 18: Buttons On The P2-Rc Remote Controller

    Chapter 1 Components Buttons on the P2-RC Remote Controller (9) Capture button (1) LCD (2) Backlight control dial (6) Filter cube switching button (4) Fine/coarse focus switching button (4) Fine/coarse focus switching button (8) Z-reset button (3) Focus knob FL Cube FL Cube (3) Focus knob (7) LCD display switching...

  • Page 19: Compact System

    Chapter 1 Components Compact System Names of the Devices and Operation Sections DS camera head P2-TL100 Trinocular Tube L Optical path switching lever Zooming knob P2-FMDN Focus Mount SMZ18 Zooming Body Coarse focus knob Fine focus knob Objective P-PS32 Plain Stand Clamp tool Diopter adjustment ring Zooming knob...
  • Page 20 Chapter 1 Components ☆☆☆...

  • Page 21: Microscopy Procedures

    Chapter Microscopy Procedures This chapter describes the microscopy procedures listed below. The devices used in the descriptions are based on the configuration examples given in the previous chapter. Procedure 1: Procedure for microscopy under episcopic illumination Procedure 2: Procedure for microscopy under diascopic illumination Procedure 3: Procedure for epi-fluorescence microscopy It is assumed that all required components are attached to the microscope with all necessary cables properly connected.

  • Page 22: Operation Flowchart

    Chapter 2 Microscopy Procedures Operation Flowchart Microscopy under episcopic illumination or diascopic illumination Turn on the power. Set a sample. Bring an objective into the optical path. Turn on the illumination and adjust the brightness. Set the optical path to 100% to the binocular part.

  • Page 23: Procedure For Microscopy Under Episcopic Illumination

    Chapter 2 Microscopy Procedures Procedure for Microscopy under Episcopic Illumination This section describes the procedure for performing microscopy under episcopic illumination using the SMZ18 Zooming Body combined with the P2-CI Coaxial Epi Illuminator. Turn on the power. Turn on the P2-CTLB Control Box by pressing the power switch to the “|”...

  • Page 24: Adjust The Diopter

    Chapter 2 Microscopy Procedures Turn on the illumination and adjust the brightness. Refer to the instruction manual for the C-FLED2 LED Light Source for Fiber Illuminator. Aperture diaphragm The SMZ18 Zooming Body is equipped with an aperture diaphragm. Turn the aperture diaphragm dial counterclockwise (Ο...
  • Page 25 Chapter 2 Microscopy Procedures Adjust the interpupillary. Adjust to match the distance between your right and left eyes. Look into both eyepieces and move the binocular part of the tube so that the right and left fields of view merge into a single circle. This adjustment is required for each user.
  • Page 26 Chapter 2 Microscopy Procedures Turn off the power after completing microscopy. Turn off the P2-CTLB Control Box by pressing the power switch to the “O” position. Check that the power LED on the control box turns off. Power LED: Off Also turn off the LED light source for fiber illuminator.

  • Page 27: Procedure For Microscopy Under Diascopic Illumination

    Microscopy Procedures Procedure for Microscopy under Diascopic Illumination This section describes the procedure for microscopy under diascopic illumination using the SMZ25 Zooming Body combined with the P2-DBL LED Diascopic Illumination Base. For the same operations as in “2 Procedure for Microscopy under Episcopic Illumination,” details are omitted.
  • Page 28 ON/OFF ON/OFF ON/OFF ON/OFF Aperture diaphragm LED ON/OFF Diascopic LED The SMZ25 Zooming Body is equipped with an indicator brightness control aperture diaphragm. Turn the aperture diaphragm button dial counterclockwise (O side) to fully open the Remote on/brightness control aperture. (For details about using the aperture diaphragm, see Chapter 3, “10 Using the Aperture...
  • Page 29 0.1x. When a step size is selected, the Zoom operation corresponding step LED indicator lights. (See Chapter 4, “1.2 SMZ25 Zooming Body.”) Turn off the power after completing microscopy. Turn off the P2-CTLA Control Box by pressing the power switch to the “O”...

  • Page 30: Procedure For Epi-Fluorescence Microscopy

    Chapter 2 Microscopy Procedures Procedure for Epi-fluorescence Microscopy After locating a target in a sample in “3 Procedure for Microscopy Under Diascopic Illumination” (steps 1 to 10), follow the procedure below: Turn off the diascopic LED illumination. Press the diascopic LED ON/OFF button on the remote controller to turn off the LED illumination.
  • Page 31 Chapter 2 Microscopy Procedures Adjust the angle of the light shielding plate. Change the angle of the light shielding plate to 45° to prevent light reflected from the objective from entering your eyes. Adjusting the angle of the light shielding plate Turn on the HG precentered fiber illuminator and adjust the brightness.
  • Page 32 Chapter 2 Microscopy Procedures ☆☆☆...

  • Page 33: Specific Operations

    Chapter Specific Operations This chapter describes how to work with this microscope system in order to observe samples. For details about using the individual devices, see Chapter 4.

  • Page 34: Turning On The Power

    Chapter 3 Specific Operations Turning On the Power When using this microscope system, power to the following devices must be turned on:  P2-CTLA Control Box  P2-CTLB Control Box  P2-DBL LED Diascopic Illumination Base 12 V AC adapter For a system configuration where a control box has been connected, the P2-DBL LED Diascopic Illumination Base need not be connected to a power supply.

  • Page 35: Adjusting The Brightness

    Chapter 3 Specific Operations Adjusting the Brightness The illumination brightness can be adjusted for the following devices. Turn the brightness control dial to adjust the brightness to a comfortable level. Depending on the system configuration, some devices support remote control of the brightness. For details, see the sections in Chapter 4 describing how to use the individual devices.

  • Page 36: Switching The Optical Path Of The Tube

    Chapter 3 Specific Operations Switching the Optical Path of the Tube For observing a specimen using the eyepiece, switch the optical path to the binocular part. For imaging using the DS camera head, switch the optical path to the vertical tube. Use the optical path switching lever of the tube to switch the optical path.

  • Page 37: Adjusting The Angle Of Depression

    Magnification them into focus separately. decrease [Image zooming] SMZ18 Zooming Body: Zooming knob on the zooming body SMZ25 Zooming Body: Zoom buttons on the remote controller or the right pedal of the Magnification foot switch increase FL Cube...

  • Page 38: Adjusting The Interpupillary Distance

    Chapter 3 Specific Operations Adjusting the Interpupillary Distance Adjust to match the distance between your right and left eyes. This adjustment is required for each user. Look into both eyepieces and move the binocular part of the tube so that the right and left fields of view merge into a single circle. Adjusting the interpupillary distance...

  • Page 39: Focusing On The Sample

    Chapter 3 Specific Operations Focusing on the Sample Focus adjustment of the sample is performed by the rotation of the focus knobs on the focus unit or focus mount. When the remote controller or foot switch is connected, focusing can be adjusted using the focus knobs of the remote controller or the foot pedals on the left side of the foot switch.
  • Page 40 Chapter 3 Specific Operations Check that the optical path of the tube is 100% to the binocular part. Using coarse control, lower the zooming body to a position near the working distance. [Working distances of objectives] 0.5x： 71 mm 1x： 60 mm 1.6x：...

  • Page 41: Changing The Magnification

    Useful functions for image zooming When using the SMZ25 Zooming Body, the magnification step size can be switched by pressing the zoom step switching button of the remote controller. The magnification can also be switched automatically so that the field of view before switching is maintained when the objective is switched (ALZ function).

  • Page 42: Switching Objectives (Using The P2-Rni2 Intelligent Nosepiece)

    Chapter 3 Specific Operations Total magnification The total magnification of the microscope is determined from the formula below: Total magnification = zoom magnification x object’s magnification x eyepiece’s magnification  When using a vertical tube, replace the eyepiece’s magnification with the C mount adapter lens’ magnification to calculate the total magnification.

  • Page 43: Shifting A Sample

    Chapter 3 Specific Operations Shifting a Sample To view another part of a sample during microscopy, use the following procedure to shift the sample. Shifting the Stage in the X and Y Directions (P-SXY64 XY Stage) Turn the XY shift knob of the P-SXY64 XY Stage mounted instead of the stage plate to shift the sample on the glass plate and change the part to be observed.

  • Page 44: Using The Aperture Diaphragm

    Chapter 3 Specific Operations Using the Aperture Diaphragm The SMZ18 and SMZ25 Zooming Bodies contain an aperture diaphragm. Turn the aperture diaphragm dial to adjust the aperture. The aperture can be adjusted in the range from fully open to 3...

  • Page 45: Using Diascopic Illumination

    Chapter 3 Specific Operations Using Diascopic Illumination The microscope system enables diascopic illumination when used with the devices listed below. All of the devices use LEDs as the light source and provide uniform illumination. The image to be observed results from diascopic illumination which passes through the sample and enters the objective.

  • Page 46: Using Epi-Fluorescence Illumination

    Chapter 3 Specific Operations Using Epi-Fluorescence Illumination Epi-fluorescence microscopy used for observing fluorescence images requires an optical element such as a fluorescence filter, and a brighter light source including mercury lamp. In this microscope system, state detection or motorized-type epi-fluorescence illumination is available. With a filter cube attached to the device, the filter cube is brought into the optical path by the rotation of the turret, resulting in the generation of epi-fluorescence illumination.

  • Page 47: Selecting A Filter Cube

     Locating a target in the sample For epi-fluorescence microscopy, Nikon recommend locating a target with bright-field diascopic illumination (OCC illumination) and then switching to epi-fluorescence rather than emitting strong excitation light to the sample from the beginning.

  • Page 48: Capturing Images

    When using diascopic illumination, first capture an image of the top surface of the stage glass. When using episcopic illumination, first capture an image of any white subject. Then, press the WB button to execute white balance adjustment. (For fluorescent photomicrography, Nikon recommends adjusting the white balance under normal bright-field microscopy conditions before capturing images.) Adjust the position of the sample.

  • Page 49: Tips On Microscope Settings For Photomicroscopy

    Chapter 3 Specific Operations Tips on Microscope Settings for Photomicroscopy 15.2  Confirming the photomicrographic range The image on the monitor represents the photomicrographic range.  Adjusting to eliminate extraneous light Cover the eyepiece with a piece of cloth or similar item. ...

  • Page 50: Internally Saved Information

    Chapter 3 Specific Operations Internally Saved Information In stereo microscopes, the following information saved in the control box is read and used. The lower limit and the escape distance are previously set when the product is shipped. Other items are set to OFF or nothing is set. When using a motorized system or intelligent (status detection) system for the first time, set the items as required by following the specified procedures.

  • Page 51: Functions And Operations Of The Devices

    Chapter Functions and Operations of the Devices This chapter describes the main functions and how to use the devices.

  • Page 52: Zooming Body

    Chapter 4 Functions and Operations of the Devices Zooming Body Two types of zooming bodies are available: SMZ18 Zooming Body for manual zoom operations and SMZ25 Zooming Body for motorized zoom drive. SMZ18 Zooming Body This model, which offers high NA, is designed for manual zoom operations.

  • Page 53: Smz25 Zooming Body

    Zoom control is performed by the P2-CTLA Control Box. Control signals are sent to the SMZ25 via a motorized focus unit or relay box. The zoom information is sent to the control box via a motorized focus unit or relay box, and then transmitted to the DS-L3 DS Camera Control Unit and personal computer.
  • Page 54 Chapter 4 Functions and Operations of the Devices Useful zoom operation functions (ALZ function) When the objective is switched, the zoom magnification is automatically changed so that the field of view is maintained as it was. This prevents the target image from moving or disappearing and FL Cube FL Cube makes it possible to observe the image while increasing or...

  • Page 55: Focus Units

    The P2-FU Focus Unit consists of a square-type support and a focus mount with the coaxial coarse/fine focusing system. The focus mount is attached with the SMZ18 or SMZ25 Zooming Body P2-FU Focus Unit through the P2-RNI2 Intelligent Nosepiece or the P2-FM Focus (square support) Mount Adapter.

  • Page 56: P2-Mfu Motorized Focus Unit

    The P2-MFU Motorized Focus Unit consists of a square-type support and a focus mount with the motorized focusing system. The focus mount is attached with the SMZ18 or SMZ25 Zooming Body through the P2-RNI2 Intelligent Nosepiece or the P2-FM Focus Mount Adapter. The square support is attached with the...
  • Page 57 Chapter 4 Functions and Operations of the Devices Focus knobs on the remote controller Turning the knob backward: Upward movement of the focus mount Turning the knob forward: Downward movement of the focus mount  Focusing stroke: 100 mm Pedals of the foot switch Left rear pedal: Upward movement of the focus mount Left front pedal: Downward movement of the focus mount For details about the pedal operations, see “10.2 AZ-FSW Foot Switch”...
  • Page 58 Functions and Operations of the Devices Focus knob of the P2-MFU Motorized Focus Unit The focus knob of the P2-MFU Motorized Focus Unit attached with a cover cannot be used. This knob is to be used by Nikon during maintenance.

  • Page 59: P2-Fmdn Focus Mount

    Chapter 4 Functions and Operations of the Devices P2-FMDN Focus Mount The P2-FMDN Focus Mount is used exclusively for the SMZ18. Adjust the focus using the focus knob. Focus mount The focus mount can be attached to various stands having a support of 32 mm diameter.

  • Page 60: Nosepiece

    Chapter 4 Functions and Operations of the Devices Nosepiece P2-RNI2 Intelligent Nosepiece The P2-RNI2 Intelligent Nosepiece can be attached with two objectives. The attached objectives can be switched by turning the nosepiece. Nosepiece ■ Stereoscopic view and vertical view An intelligent nosepiece has two click positions for each objective.

  • Page 61: Objectives

    Chapter 4 Functions and Operations of the Devices Objectives The microscope system supports the following objectives. Attach the objectives to the zooming body or intelligent nosepiece. Model name P2-SHR Plan Apo 0.5X P2-SHR Plan Apo 1X P2-SHR Plan Apo 1.6X P2-SHR Plan Apo 2X Magnification 0.5x...

  • Page 62: Bases, Stand, And Stages

    Chapter 4 Functions and Operations of the Devices Bases, Stand, and Stages Bases consist of various focus stands in combination with the focus units. The base models include a base without a built-in diascopic illumination system and a base with a built-in optical system for diascopic illumination. A stand type is also available where a focus mount is connected to the support of the stand.

  • Page 63: P-Ps32 Plain Stand

    Chapter 4 Functions and Operations of the Devices P-PS32 Plain Stand This stand consists of a microscope system with the SMZ18 Zooming Body attached with the P2-FMDN Focus Mount. It is equipped with a stage plate having black and milky white planes.

  • Page 64: P-Sxy64 Xy Stage

    Chapter 4 Functions and Operations of the Devices P-SXY64 XY Stage This stage shifts the glass plate in the X and Y directions when the X/Y shift knobs are turned. Attach this stage instead of the base or the stage plate on the stand. The glass plate measures 215 mm x 154 mm x 5 mm and is detachable.

  • Page 65: Tubes

    Chapter 4 Functions and Operations of the Devices Tubes This microscope system can be used with the tubes listed in the table below. Attach the 10x, 15x, 20x, or 30x eyepiece to the binocular sleeve. Then, attach a camera such as a DS camera head to the vertical tube using an adapter.

  • Page 66: Episcopic Illuminator

    P2-CI Coaxial Epi Illuminator recommended zoom magnification are shown below. Compatible objectives and recommended zoom magnification Zoom magnification Objective SMZ18 Zooming Body SMZ25 Zooming Body P2-SHR Plan Apo 0.5X 3.8x - 2.5x - P2-SHR Plan Apo 1X 4x - 4x - P2-SHR Plan Apo 1.6X...

  • Page 67: C-Fdf Flexible Double Arm Fiber Illumination Unit

    Chapter 4 Functions and Operations of the Devices C-FDF Flexible Double Arm Fiber Illumination Unit This illuminator is a fiber connected to the C-FLED2 LED Light Source for Fiber Illuminator for illuminating samples. To C-FLED2 LED Light Source for Two fibers are held by the C-FIDH Fiber Holder. The orientation of Fiber Illuminator the holder’s arm can be adjusted so that the tips of the fibers face the samples for illumination.

  • Page 68: Diascopic Illuminator

    Chapter 4 Functions and Operations of the Devices Diascopic Illuminator The primary diascopic illuminator for this microscope system consists of an illumination base equipped with an illuminating optical system. A dark-field illuminator and polarizing illuminator are also available. P2-DBL LED Diascopic Illumination Base LED brightness control selection switch INPUT...
  • Page 69 Chapter 4 Functions and Operations of the Devices  Prism sheet The LED diascopic illumination base contains a prism sheet to reduce shading. When using the 1.6x or 2x objective, push in the prism sheet IN/OUT lever. When using the 0.5x or 1x objective, pull out the lever. Compatible objectives and recommended zoom magnification Zoom magnification Objective...

  • Page 70: P2-Dbf Fiber Diascopic Illumination Base

    Chapter 4 Functions and Operations of the Devices P2-DBF Fiber Diascopic Illumination Base This illumination base contains an optical system for episcopic illumination. Since the base supports OCC illumination, high-contrast observation of transparent samples is enabled. The Fiber C-FLED2 LED Light Source for Fiber Illuminator is used as the light source.

  • Page 71: P-Df Led Dark Field Unit

    Chapter 4 Functions and Operations of the Devices Tap holes for attaching accessories 20 100 The base’s top board has tap holes for attaching various 4-M5 accessories. Tap holes are not penetrating to Depth 7 prevent ingress of solution into the base. 2-M4: Positioned under the stage plate attachment section of the base.

  • Page 72: Epi-Fluorescence Attachment

    Chapter 4 Functions and Operations of the Devices Epi-Fluorescence Attachment The microscope system can be used with the status detection model P2-EFLI Epi Fluorescence Attachment and Motorized P2-EFLM Epi Fluorescence Attachment. P2-EFLI Epi Fluorescence Attachment This illuminator is attached between the zooming body and tube. BIN O A HG precentered fiber illuminator is used as the light source.

  • Page 73: P2-Eflm Motorized Epi Fluorescence Attachment

    Also, duplicated images may be produced by internal half mirror. OCC light shielding plate When using an epi-fluorescence attachment with a diascopic illumination base, Nikon recommends closing the OCC light shielding plate on the dia-illumination completely. This can decrease background noise caused by intrinsic fluorescence in the diascopic illuminator.
  • Page 74 Also, duplicated images may be produced by internal half mirror. OCC light shielding plate When using an epi-fluorescence attachment with a diascopic illumination base, Nikon recommends closing the OCC light shielding plate on the dia-illumination completely. This can decrease background noise caused by intrinsic fluorescence in the diascopic illuminator.

  • Page 75: Remote Control Device

    Chapter 4 Functions and Operations of the Devices Remote Control Device A remote controller, foot switch, and photo release are available as remote control devices of the microscope system. P2-RC Remote Controller 10.1 The P2-RC Remote Controller is used mainly for focus and zoom operations. The remote controller is attached to the REMOTE connector of the P2-CTLA Control Box.
  • Page 76 ALZ button Activates the ALZ function. To deactivate the ALZ function, press the button again. For the ALZ function, see “ Useful zoom operation functions (ALZ function)” in “1.2 SMZ25 Zooming Body” in this chapter. (18) ALZ ON/OFF indicator The LED lights when the ALZ function is activated.

  • Page 77: Az-Fsw Foot Switch

    Chapter 4 Functions and Operations of the Devices  LCD indications 1st page 1: Total magnification (zoom magnification x objective magnification x eyepiece magnification) (Example) [MAG: 236.25x] 2: Zoom magnification (Example) [ZOOM: 15.75x] 3: Objective magnification (Example) [OBJ: 0.75x] 4: Eyepiece magnification (Example) [EP10x] 5: Diascopic LED light intensity (Example) [LEDDIA ...
  • Page 78  The speed changes based on the magnification and nosepiece address. (See the table below.)  If a nosepiece address has not been set, the unit moves at the maximum speed. Coarse SMZ25 0.63 to 5.0 5.1 to 10.0 10.1 to 15.75...

  • Page 79: Az-Pcr Photo Release

    Chapter 4 Functions and Operations of the Devices AZ-PCR Photo Release 10.3 The AZ-PCR Photo Release is a foot switch for capturing images Connection cable using the camera connected to the microscope. The photo release is connected to the PHOTO connector of the AZ-FSW Foot Switch.

  • Page 80: Control/Communications Device And Relay Device

    Chapter 4 Functions and Operations of the Devices Control/Communications Device and Relay Device P2-CTLB Control Box 11.1 Power LED Power switch P2-CTLB Control Box When electrically connected to the following devices, the P2-CTLB Control Box can detect the device states and handle communications: Device Operation...
  • Page 81 Chapter 4 Functions and Operations of the Devices  Connector names and connection destinations The figure below shows the connectors on the rear of the control box and the connection destinations: (Connect to the USB port of a personal computer or the DS-L3.) LEDDIA (Connect to the LED light control terminal of the P2-DBL LED...

  • Page 82: P2-Ctla Control Box

    P2-RC Remote Controller Communications (10) AZ-FSW Foot Switch Detects the switch states, moves the P2-MFU Motorized Focus Unit vertically, and adjust the magnification of the SMZ25 Zooming Body. (11) AZ-PCR Photo Release Detects the switch states and captures images. (12) C-HGFIE HG Precentered Fiber Illuminator Switches the ND filter and opens/closes the shutter.

  • Page 83: P2-Rly Relay Box

    P2-EFLM Epi Fluorescence Attachment.) information signals are relayed. ZOOM (Connect to the P2-RNI2 Intelligent Nosepiece.) ZOOM (Connect to the SMZ25/SMZ18 Zooming Body.) P2-RLY Relay Box P2-RLYC Relay Cable 11.4 To the RELAY For systems where the P2-RLY Relay Box has not been...
  • Page 84 Chapter 4 Functions and Operations of the Devices ☆☆☆...

  • Page 85: Assembling

    Chapter Assembling This chapter contains the SMZ18/SMZ25 system configuration diagrams and lists of the system components, and explains how to assemble the systems. Before starting assembling, read the appropriate notes such as CAUTION “11 Cautions on assembling and installing the product”...

  • Page 86: System Configuration

    P2-FM focus mount adapter P2 RLYC relay cable Signal cable P2-RNI2 Signal cable intelligent nosepiece ZOOM* SMZ18 SMZ25 Signal cable zooming body zooming body ZOOM* P2-CTLA or P2-CTLB control box Objective P2-RLY relay box (Connectors: NP, ZOOM and FL) 1/4 lambda...
  • Page 87 Chapter 5 Assembling Control Box P2-RC remote controller P2-CTLB P2-CTLA control box control box (Connectors: HGFIE, USB, LEDDIA, (Connectors: FOOT, REMOTE, HGFIE, RELAY, and DC24V IN) USB, LEDDIA, STAND, and DC24V IN) AZ-FSW foot switch 24V AC Adapter and power cable AZ-PCR photo release Other Illuminators...

  • Page 88: Device List

    Illumination Remarks System Episcopic and Epi- Illumination fluorescence Illumination  SMZ18 Zooming Body   Main Body SMZ25 Zooming Body P2-MFU Motorized Focus Unit   Focus Unit or P2-FU Focus Unit Focus Mount  P2-FMDN Focus Mount Focus Mount...

  • Page 89: Assembly Procedures

    Assembling Assembly Procedures The SMZ18 and SMZ25 stereo microscopes are system microscopes that can be configured in a variety of ways. This chapter describes how to assemble the following three systems.  Episcopic illumination system (called the “EPI System” in this chapter) ...
  • Page 90 Chapter 5 Assembling Place the focus unit in the recession of the polystyrene piece as show in the figure. Positioning pins Fitting the focus unit Hold the base with the two base side positioning holes aligned with the two positioning pins on the focus unit, screw the four M8 hexagon socket head bolts (supplied with the focus mount) from the bottom of the base and tighten firmly using a hex...
  • Page 91 Chapter 5 Assembling Glass stage plate handling precautions Be very careful when handling the glass stage plate. Before attaching the glass stage plate, attach all other components in order to avoid the risk of breaking the glass during assembly. Loosen the M4 set screw for the stage plate in the front center of the base using a hex driver (nominal designation: 2).
  • Page 92 Chapter 5 Assembling Attach the intelligent nosepiece or focus mount adapter. Attach the intelligent nosepiece or focus mount adapter to the focus unit. Both the intelligent nosepiece and focus mount adapter are attached to the focus unit in the same manner. The following figure shows an example of attaching the intelligent nosepiece to the P2-FU Focus Unit.
  • Page 93 Signal cable Attach the zooming body. Attach the SMZ18 or SMZ25 Zooming Body to the intelligent nosepiece or focus mount adapter. Both zooming bodies are attached to the intelligent nosepiece or focus mount adapter in the same manner. The following figure shows an example of attaching the SMZ18 Zooming Body to the intelligent nosepiece.
  • Page 94  Connecting signal cables The signal cable for the SMZ25 comes from the left side of the zooming body. In case of the SMZ18, connect the signal cable supplied with the SMZ18 to the round connector on the left side of the zooming body.
  • Page 95 4N75 INSPECTION EQUIPMENT ZOOM SMZ25 MADE IN JAPAN the relay box is explained in Step [5]) Bring out the cable from the SMZ zooming body along the notch on the focus unit cover and connect to the connector “ZOOM.”...
  • Page 96 Chapter 5 Assembling Clamp the relay box cable using the arm clamps. Clamps Clamping a cable Place the relay box arms over the focus unit as shown in the figure, align the screw holes of the arm with the two screw holes at the back of the focus unit, and attach it by tightening the M5 hexagon socket head bolts supplied with the relay box using a hex driver (nominal designation: 4).
  • Page 97 Chapter 5 Assembling Attach the coaxial epi illuminator. (for the EPI System) Attach the coaxial epi illuminator to the zooming body. Completely loosen the M4 set screw for the illuminator at the top front of the zooming body using a hex driver (nominal designation: 2). Align and fit the round dovetail at the bottom of the coaxial epi illuminator with the round dovetail of the zooming body and tighten the fixing screw.
  • Page 98 Chapter 5 Assembling Attach the epi fluorescence attachment. (for the DIA + EPI-FL System) Attach the P2-EFLM Motorized Epi Fluorescence Attachment or the P2-EFLI Epi Fluorescence Attachment to the zooming body. Both epi fluorescence attachments are attached to the zooming body in the same manner. Completely loosen the M4 set screw for the illuminator at the top front of the zooming body using a hex driver (nominal designation: 2).
  • Page 99 Chapter 5 Assembling Remove the filter cube replacement hole cover on the right side of the epi fluorescence attachment (as viewed from the front). Push it upward to remove. Removing the filter cube replacement hole cover Align and insert the dovetail groove of the filter cube to the internal dovetail until it reaches the limit.
  • Page 100 Chapter 5 Assembling  Attaching a light shielding plate When using a focus mount, attach the light shielding plate to the zooming body, and when using an intelligent nosepiece, attach it to the bottom of the intelligent nosepiece.  Attachment to the zooming body Fit the ring of the light shielding plate to the periphery of the dovetail attachment adapter attached to the lower part of the zooming body, and firmly tighten the M2.5 hexagon...
  • Page 101 Chapter 5 Assembling Attach the tube. Attach a tube to be used to the epi illuminator or epi fluorescence attachment. All types of tubes can be attached to the illuminator in the same manner. Completely loosen the M4 set screw for the tube at the top front of the illuminator using a hex driver (nominal designation: 2).
  • Page 102 Chapter 5 Assembling Attach the objective. Attach the objective to be used to the zooming body or intelligent nosepiece. Up to two intelligent nosepieces can be attached. Both objectives are attached to the zooming body or intelligent nosepiece in the same manner. The following figures show an example of attaching a 2x objective to the intelligent nosepiece.
  • Page 103 Chapter 5 Assembling  Detaching the objective Loosen the objective fixing screw. Hold and rotate the objective clockwise (as viewed from the top) and align the mark (white dot) on top of the objective with the objective fixing screw position. Mating of the dovetail can be released at this position.
  • Page 104 Chapter 5 Assembling WARNING Before connecting the power cord To prevent electric shock, be sure to turn off the power switch (press it to the “” position) at the front of the control box before connecting the power cord to the adapter. Connect the Remote Control Devices.

  • Page 105: Compact System Assembly

    Chapter 5 Assembling Compact System Assembly Install the stand. Select a location free of vibration. Install a P-PS32 Plain Stand on a level surface.  Attaching a support to the stand Fit the support to the stand and tighten the clamp screw on the rear side using a hex driver supplied with the support (The hex driver is inserted on the top of the support).
  • Page 106 Chapter 5 Assembling Attach the focus mount. Attach the P2-FMDN Focus Mount to the stand. Fit the focus mount in the stand from the top of the support and tighten the fixing knob on the rear side. Attaching the focus mount by tightening the knob Attach the zooming body Attach the SMZ18 Zooming Body to the focus mount.
  • Page 107 Chapter 5 Assembling Attach the tube. Attach a tube to be used to the zooming body. The procedure for attaching a tube to the zooming body is the same as the procedure for attaching a tube to the fluorescence attachment or epi-fluorescence attachment. See “3.1, Step 8 Attach the tube.”...

  • Page 108: Assembly Of Other Devices (Optional)

    Chapter 5 Assembling Assembly of Other Devices (Optional) Attach the C-FDF Flexible Double Arm Fiber Illumination Unit. This unit can be attached to any kinds of the bases or the stand. Fixing Fixing Attach a base of the C-FIDH Fiber Holder to the right bolt bolt and left rear sides of the base.
  • Page 109 Chapter 5 Assembling Directly placing the polarizer When the LED diascopic illumination base is in use, the polarizer can be removed from the polarizer attachment and can be individually attached to the diascopic illumination base. Fit it under the stage plate of the diascopic illumination base.
  • Page 110 Chapter 5 Assembling Attach the C-SSL DIA Sliding Stage. Attach the C-SSL DIA Sliding Stage to the P2-PB Plain Base, P2-DBL LED Diascopic Illumination Base or P2-DBF Fiber Diascopic Illumination Base. Loosen the M4 set screw for the stage plate on the base using a hex driver (nominal designation: 2) to detach the stage plate.

  • Page 111: Troubleshooting

    If you detect problems that are not listed in the table or the problem still persists even after the indicated measures are taken, turn off the device and contact your nearest Nikon representative.

  • Page 112: Image Viewing

    Chapter 6 Troubleshooting Image Viewing Problem Cause Measure Devices are not attached correctly. Attach the devices correctly. The optical path switching lever of the tube is Position the lever correctly. positioned partway. Part of the field of view is missing. Firmly turn the nosepiece to the click position for The nosepiece is not turned to the click The field of view is...

  • Page 113: Motorized Devices

    Chapter 6 Troubleshooting Motorized Devices Problem Cause Measure The product cannot be The power cable is not connected. Connect the power cable properly. turned on. Check the connections between the AC adapter The power of the P2-CTLA Control Box is not and the P2-CTLA Control Box, and then move the Operating the remote turned on or the AC adapter is not connected.
  • Page 114 Chapter 6 Troubleshooting ☆☆☆...

  • Page 115: Maintenance And Storage

    Chapter Maintenance and Storage This chapter describes how to maintain and store the product.

  • Page 116: Cleaning

    Chapter 7 Maintenance and Storage Cleaning Clean and disinfect the microscope and lenses as described in the procedures below.  Tools used for cleaning  Blower  Soft brush  Soft cotton cloth, lens tissue, gauze, etc.  Pure alcohol (ethyl or methyl alcohol), medical alcohol CAUTION ...

  • Page 117: Disinfecting The Product

     Switch off the device (press the switch to the “O” position). If the device is warm, wait for it to cool before covering it with a cover. Periodic Inspection (Charged) To maintain the performance of this product, Nikon recommends periodic inspection (chargeable service). Contact your nearest Nikon representative for details.
  • Page 118 Chapter 7 Maintenance and Storage ☆☆☆...

  • Page 119: Specifications

    Chapter Specifications...

  • Page 120: Microscopy (Principles)

    (such as magnifications or Z-position information) is not guaranteed. These values are used as reference and include a margin of error. Performance Properties  Stereo microscope Name Zooming body Zooming body Model SMZ18 SMZ25 Zoom ratio 18:1 25:1 Magnification 0.75 to 13.5x 0.63 to 15.75x 0.15 0.156 (For 1x objective and 13.5x zoom)
  • Page 121 Chapter 8 Specifications  Base/stand Name Plain Base LED Diascopic Fiber Diascopic Plain Stand Illumination Base Illumination Base Model P2-PB P2-DBL P2-DBF P-PS32 Tap hole With tap holes for With tap holes for With tap holes for With tap holes for accessories accessories accessories...
  • Page 122 Chapter 8 Specifications  Eyepiece Name Eyepiece Eyepiece Eyepiece Eyepiece Model C-W10XB C-W15X C-W20X C-W30X Magnification Field number 12.5  Episcopic illuminator Name Coaxial Epi Illuminator Flexible Double Arm Ring Fiber Illumination LED Ring Illumination Fiber Illumination Unit Unit Unit Model P2-CI C-FDF...
  • Page 123 Chapter 8 Specifications  Relay device Name Relay Box Relay Cable Model P2-RLY P2-RLYC  Relay of signals for motorized control of a device or  Relay of status detection signals with the SMZ18 Function, etc. detection of the device status Zooming Body and RELAY of the P2-CTLB Control Box connected ...

  • Page 124: Physical Properties

    Chapter 8 Specifications Physical Properties Research stereo microscope SMZ25/SMZ18 Operating conditions Temperature: 0°C to +40°C Humidity: 60% RH max. (no condensation) Altitude: 2,000 m max. Pollution level: Degree 2 Overvoltage Category: Category II Short-term temporary overvoltage: 1440 V (5 sec. max.) Long-term temporary overvoltage: 490 V (over 5 sec.)

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