Procedure For Microscopy Under Diascopic Illumination - Nikon SMZ25 Instruction Manual

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Procedure for Microscopy under Diascopic Illumination

This section describes the procedure for microscopy under diascopic illumination using the SMZ25 Zooming Body combined
with the P2-DBL LED Diascopic Illumination Base.
For the same operations as in "2 Procedure for Microscopy under Episcopic Illumination," details are omitted.
1
Turn on the power.
Turn on the P2-CTLA Control Box by pressing the power
switch to the "|" position.
Check that the power LED on the control box lights.
P2-MFU Motorized Focus Unit
When the power is turned on, the motorized focus
unit is initialized as follows:
(1) The focus mount rises to the upper sensor
limit.
(2) This is followed by downward movement to the
position when power was turned on.
* Other motorized devices also perform internal
initialization.
Power supply to the P2-DBL LED
Diascopic Illumination Base
For a system configuration where a control box has
been connected, the control box supplies power to
the P2-DBL LED Diascopic Illumination Base. The
P2-DBL therefore need not be connected to a
power supply. (See Chapter 8, "8.1 P2-DBL LED
Diascopic Illumination Base.")
Power supply to the DS-L4 camera control
unit
When the DS-L4 camera control unit is connected,
turn on the control box and then the DS-L4.
Forced stop of motorized movement
The motorized focus unit is equipped with the STOP switch on the left side. Pressing the switch causes motorized
movement to be forcibly stopped. To resume the motorized movement, perform the following steps:
1. Press the STOP switch again.
2. Turn off the power by pressing the power switch of the P2-CTLB Control Box to the "" position.
3. Turn on the power by pressing the power switch to the "|" position. (After the power on, initialization at power on
is automatically performed.)
2
Set a sample.
→ Step 2 in "2 Procedure for Microscopy Under Episcopic Illumination"
Empty cube
When an epi-fluorescence attachment is mounted, check that the empty cube of the attachment is in the optical
path.
Chapter 2
Microscopy Procedures
13
STOP switch
Power LED: On
Power switch: "|"
position
Power on