Capturing Images; Procedure For Capturing Images - Nikon SMZ25 Instruction Manual

15

Capturing Images

The microscope system can capture images of the sample being observed by attaching a camera such as a DS camera to
the vertical tube.

Procedure for Capturing Images

15.1
Follow the procedure below to capture images. For the camera settings, refer to the instruction manual supplied with the
DS-L4.
Using the DS-L4
Specify the following items beforehand.
• Folder to save
• Name of the save file (or select Auto.)
• File format and file size of the save file
• Date and destination of the data
(1) Adjust illumination of the microscope correctly and
focus the sample image.
(2) Adjust the mounting orientation of the DS camera.
For details about attaching the DS camera, see [5] Attach a
DS camera in Chapter 3, "3.3 Assembly of Other Devices
(Optional)."
Since shifting a sample horizontally causes the image on
the monitor to move horizontally, loosen the attachment
guide fixing screw and adjust the orientation of the DS
camera. After making the appropriate adjustments, tighten
the screw.
(3) Select the DS camera scene mode suitable for the microscopy type.
(4) Adjust the DS camera's white balance.
When using diascopic illumination, first capture an image of the top surface of the stage plate. When using episcopic
illumination, first capture an image of any white subject. Then, press the WB button to execute white balance
adjustment. (For fluorescent photomicrography, Nikon recommends adjusting the white balance under normal
bright-field microscopy conditions before capturing images.)
(5) Adjust the position of the sample.
(6) Readjust the focus on the target.
(7) Adjust the image brightness using the DS camera exposure compensation function.
(8) Press the [FREEZE] button on the DS-L4 to check the image.
(9) Save the image if acceptable.
To save the image, press the capture button on the remote controller or DS-L4, or step on the pedal of the AZ-PCR
Photo Release.
Chapter 7
Specific Operations
83
Adjusting the DS camera orientation
DS camera
C-mount adapter
Attachment guide
fixing screw
LV-TV TV Tube
Adapter