Nikon SMZ25 Instruction Manual page 135

 Filter cube
• P2-EFL GFP-B, P2-EFL GFP-L, and P2-EFL RFP Filter Cubes: Used for fluorescence observation with a given
wavelength.
• P2-EFLC Filter Cube: Used for fluorescence observation with an arbitrary wavelength when combined with arbitrary
filters, for example.
• P2-EFLBFA Filter Cube (Bright Field): Used for bright-field episcopic observation.
• Empty cube (Three cubes are supplied with an epi-fluorescence attachment): Used when performing diascopic
illumination observation using the epi-fluorescence attachment.
Light shielding plate
The device is equipped with a light shielding plate for preventing the reflection of ultraviolet light. Use the light shielding
plate when performing fluorescence microscopy. When in use, the light shielding plate can be pushed aside to positions
up to 45° horizontally to the rear. When not in use, the plate can be pushed aside to positions up to 170°.
Bright-field episcopic observation
This is useful for observing the entire image of an opaque specimen during fluorescence observation. Although the
P2-EFLBFA Filter Cube (Bright Field) contains a UV blocking filter, adjust the brightness of the illuminator so that
illumination is not too bright. A 1/4 λ plate is supplied as an option for the P2-EFLBFA to prevent in the imaging system
due to light reflected into the optical system. Attach the 1/4 λ plate to a 0.5x or 1x objective. When observing light
reflected from a specimen with a mirror-like surface, the brightness on the right optical path differs from that on the left
optical path. Also, duplicated images may be produced by the internal half mirror.
OCC light shielding plate
When using an epi-fluorescence attachment with a diascopic illumination base, Nikon recommends closing the OCC light
shielding plate on the dia-illumination completely. This can decrease background noise caused by intrinsic fluorescence in
the diascopic illuminator.
 1/4 λ plate
When using a P2-EFLBFA Filter Cube (Bright Field) with a
0.5x or 1x objective, an optional 1/4 λ plate must be attached
to the tip of the objective.
1/4 λ plates are supplied to prevent flare in the imaging
system due to light reflected into the optical system.
There are the following two types of 1/4 λ plates.
• P2-CIA QL0.5X 1/4 λ plate 0.5x for coaxial epi illuminator:
Intended for 0.5x objectives
• P2-CIA QL1X 1/4 λ plate 1x for coaxial epi illuminator:
Intended for 1x objectives
When using a 0.5x objective, remove the protection ring from
the tip of the objective, and then screw in the 1/4 λ plate.
When using a 1x objective, remove the correction glass from
the tip of the objective, and then screw in the 1/4 λ plate.
The brightest illumination is generated when the white dot on
the 1/4 λ plate is at the front.
Select a position that has the optimum brightness and contrast
for observation.
For details about using the light source, refer to the instruction manual for the light source.
Chapter 8
Functions and Operations of the Devices
121
White dot
1/4 λ plate