Procedure For Microscopy Under Diascopic Illumination - Nikon SMZ25 Instruction Manual

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Procedure for Microscopy under Diascopic Illumination

This section describes the procedure for microscopy under diascopic illumination using the SMZ25 Zooming Body combined
with the P2-DBL LED Diascopic Illumination Base.
For the same operations as in "2 Procedure for Microscopy under Episcopic Illumination," details are omitted.
1
Turn on the power.
Turn on the P2-CTLA Control Box by pressing the power
switch to the "|" position.
Check that the power LED on the control box lights.
P2-MFU Motorized Focus Unit
When the power is turned on, the motorized focus
unit is initialized as follows:
(1) The focus mount rises to the upper sensor
limit.
(2) This is followed by downward movement to the
position when power was turned on.
* Other motorized devices also perform internal
initialization.
Power supply to the P2-DBL LED
Diascopic Illumination Base
For a system configuration where a control box has
been connected, the control box supplies power to
the P2-DBL LED Diascopic Illumination Base. The
P2-DBL therefore need not be connected to a
power supply. (See Chapter 4, "8.1 P2-DBL LED
Diascopic Illumination Base.")
Power supply to the DS-L3 DS Camera
Control Unit
When the DS-L3 DS Camera Control Unit is
connected, turn on the control box and then the
DS-L3.
2
Set a sample.
→ Step 2 in "2 Procedure for Microscopy Under Episcopic Illumination"
Empty cube
When an epi-fluorescence attachment is mounted, check that the empty cube of the attachment is in the optical
path.
3
Bring an objective into the optical path.
→ Step 3 in "2 Procedure for Microscopy Under Episcopic Illumination"
Chapter 2
Microscopy Procedures
13
Power LED: On
Power switch: "|"
position
Power on