General Description; Principle - Qiagen EZ2 Connect MDx User Manual

3

General Description

The EZ2 Connect MDx system is designed to perform automated isolation and purification of nucleic
acids in molecular diagnostic and/or molecular biology applications.
3.1

Principle

The EZ2 Connect MDx performs fully automated nucleic acid purification from up to 24 samples
per single run in molecular diagnostic and molecular biology applications using magnetic particle
technology. The EZ2 Connect MDx is designed to automate EZ1 DSP Kits available from QIAGEN
(in the IVD mode of the software), processing of non-DSP kits is also supported (in the Research
mode of the software).
The EZ2 Connect MDx gives the option to start a protocol either in the IVD mode of the software
(for validated IVD applications only) or in the Research mode of the software (for Molecular Biology
Applications (MBA) only). The usage of IVD protocols is only possible and strictly restricted to the
IVD mode of the software. This user manual focuses on operating the EZ2 Connect MDx in the IVD-
software mode. For detailed instructions on how to operate the EZ2 Connect MDx using the
Research mode of the software (with MBA-protocols or any customized protocols), please refer to
the user manual of the EZ2 Connect (available on the EZ2 Connect product webpage under the
Product Resources tab).
EZ2 Connect MDx is preinstalled with various protocols for processing QIAGEN EZ1 DSP and
EZ1&2 kits for purification of RNA, genomic DNA, and viral and bacterial nucleic acids, and RNA.
The user must first log-in to the software in IVD mode using the touchscreen, then scans a kit barcode
and loads labware, samples, and reagents onto the EZ2 Connect MDx worktable. The user then
closes the instrument hood and starts the protocol, which provides all necessary commands for
sample lysis and purification. A fully automated load check helps to ensure correct loading of the
worktable.
Offering an augmented user interface, users stay connected to their instrument through the built-in
screen and also remotely with a computer or a mobile device (e.g., a tablet) and the QIAsphere
App using the associated QIAsphere Connectivity Package (Configuring network and QIAsphere
Base connections and Appendix B - EZ2 accessories), enabling quick response times and the ability
to monitor runs while being away from the instrument.
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