Qiagen EZ1 Quick Start
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Quick-Start Protocol
EZ1&2™ ccfDNA Kit
The EZ1&2 ccfDNA Kit (cat. no. 954854) is shipped at room temperature (15–25°C). Upon
receipt, store all kit components dry at room temperature.
Further information
EZ1&2 ccfDNA Kit Handbook
Safety Data Sheets: www.qiagen.com/safety
Technical assistance: www.support.qiagen.com

Notes before starting

Plasma samples should be at room temperature before use. If plasma/serum was stored
frozen, thaw the samples by incubating at 37°C in a water bath.
If the sample volume is less than 2 ml, 4 ml, or 8 ml, adjust the volume with PBS to 2 ml,
4 ml, or 8 ml.

Procedure

1. Load reagent cartridges into the cartridge rack.
2. Resuspend Magnetic Bead Suspension EZ by vortexing briefly and then tap to ensure all
the liquid is at the bottom of the tube. Remove the cap from the tube with the bead
solution placed in position 11 of the EZ1&2 ccfDNA cartridge (see Figure 1).
Sample to
Insight__
: www.qiagen.com/HB-2915
November 2021

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Summary of Contents for Qiagen EZ1

  • Page 1 November 2021 Quick-Start Protocol EZ1&2™ ccfDNA Kit The EZ1&2 ccfDNA Kit (cat. no. 954854) is shipped at room temperature (15–25°C). Upon receipt, store all kit components dry at room temperature. Further information EZ1&2 ccfDNA Kit Handbook : www.qiagen.com/HB-2915  Safety Data Sheets: www.qiagen.com/safety ...
  • Page 2 Figure 1. EZ1&2 ccfDNA cartridge. 3. Transfer 900 µl Elution Buffer EZE into position 12 of the EZ1&2 ccfDNA cartridge. 4. Remove caps of all tubes and prepare the Tip Rack as follows (see Figure 2): Position 4/A: Tip holder with Filter Tip (provided) ...
  • Page 3 ® 1. Turn on the EZ2 Connect instrument. 2. Tap “DNA” on the Applications panel and select the “EZ1&2 ccfDNA Kit” and press Next. Follow onscreen instructions for selection of protocol, parameter definition, sample position selection, sample IDs, and worktable setup.
  • Page 4 ® , EZ1&2™, EZ2 ® (QIAGEN Group). Registered names, trademarks, etc. used in this document, even when not specifically marked as such, are not to be considered unprotected by law. 1126415 11/2021 HB-2891-002 © 2021 QIAGEN, all rights reserved. Ordering www.qiagen.com/shop | Technical Support support.qiagen.com | Website www.qiagen.com EZ1&2 ccfDNA Kit...

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