Adjusting The Units For Transmitted Light Observation; Centering The Condenser - Olympus GX53 Instructions Manual

4-9 Adjusting the units for transmitted light observation

a b
e
6
g
d
h
Field diaphragm image
A
Field of view of the Field of view of the
eyepiece eyepiece
B
C
D
1

Centering the condenser

1
2
3
c
4
5
Do not rotate.
6
7
8
NOTE
9
10
TIP
Set the main switch ( a or b ) of the reflected LED light source (BX3M-
PSLED) or the power supply for halogen bulb (TH44-100/TH4-200) to
(ON), and rotate the brightness control knob to set the appropriate
brightness.
For details, refer to the instruction manual for the power
supply (BX3M-PSLED) or (TH4-100/TH4-200).
Rotate the condenser height adjustment knob c to lower the
condenser to the lower limit.
Fully open the aperture diaphragm ring d (NA0.60).
Fully open the field diaphragm lever e (
Rotate the revolving nosepiece to engage the 10X objective in the
light path and place the sample on the stage to bring it into focus
approximately.
Rotate the field diaphragm lever e in the arrow direction to narrow
down the field diaphragm to within the field of view.
Rotate the condenser height adjustment knob c to bring the field
diaphragm image into focus. (Picture A)
Rotate the centering knobs (2 positions) g to adjust the field
diaphragm image so that it comes to the center of the field of view.
(Picture B)
Do not rotate the condenser clamping screw h attached
to the rear of the centering knob g by mistake.
Rotate the field diaphragm lever e to open the field diaphragm
gradually until the field diaphragm image is inscribed in the field of
view. (Picture C)
If the center of the field diaphragm image is deviated, try centering
again.
Open the field diaphragm until the field diaphragm image becomes
almost the same size (circumscribed) as the field of view. (Picture D)
As NA of this condenser is from 0.05 to 0.60, if the 40X or
higher-objective is used, the peripheral area of the field of view
becomes dark due to insufficient NA.
GX53
¦
→
).
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