Table of Contents
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BX-RFAA
U-LH100HGAPO
U-LH100G
Power Supply Unit
U-25ND6
U-25ND25
U-RSL6
U-RSL6EM
BX-RFSS
U-EXBABG
U-EXBAUB
U-EXBAUG
INSTRUCTIONS
MOTORIZED REFLECTED
FLUORESCENCE
SYSTEM
This instruction manual is for the Olympus Motorized Reflected Fluorescence System. To ensure the
safety, obtain optimum performance and to familiarize yourself fully with the use of this system, we
recommend that you study this manual thoroughly before operating the system. Retain this instruction
manual in an easily accessible place near the work desk for future reference.
A X 9 9 4 8
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Table of Contents
Related Manuals for Olympus BX-RFAA
Summary of Contents for Olympus BX-RFAA
- Page 1 FLUORESCENCE SYSTEM This instruction manual is for the Olympus Motorized Reflected Fluorescence System. To ensure the safety, obtain optimum performance and to familiarize yourself fully with the use of this system, we recommend that you study this manual thoroughly before operating the system. Retain this instruction manual in an easily accessible place near the work desk for future reference.
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Page 2: Table Of Contents
CONTENTS Correct assembly and adjustments are critical for a reflected fluorescence system to exhibit its full performance. If you are going to assemble the microscope yourself, please read chapter 9, “ASSEMBLY” (pages 29 to 35) carefully. IMPORTANT — Be sure to read this section for safe use of the equipment. — NOMENCLATURE REFLECTED FLUORESCENCE OBSERVATION PROCEDURE USING THE CONTROLS... - Page 3 OPTIONAL MODULES 23-28 6-Position Filter Slider U-RSL6 ..........................23-24 6-Position Barrier Filter Slider U-RSL6EM ...................... 25 Rectangular Field Stop Unit BX-RFSS (for exclusive use with the BX-RFA/RFAA)..26 27-28 Exciter Balancers U-EXBABG/EXBAUB/EXBAUG (for exclusive use with the BX-RFA/RFAA) .... ASSEMBLY 29-35 —...
- Page 4 6. Do not disassemble the power supply unit because it contains high voltage parts inside. 7. Always use the power cord provided by Olympus. If no power cord is provided, please select the proper power cord by referring to the chapter “PROPER SELECTION OF THE POWER SUPPLY CORD” at the end of this instruction manual. If the proper power cord is not used, product safety and performance cannot be guaranteed.
- Page 5 If the burner is used far beyond the service life, the distortion will accumulate in the glass tube, which may explode in worst case. Applicable Intermediate Attachment The BX-RFAA motorized reflected fluorescence illuminator can be used in two-stage stacked configuration by attaching an intermediate attachment on it.
- Page 6 Caution If the system is used in a manner not specified by this manual, the safety of the user may be imperiled. In addition, the equipment may also be damaged. Always use the equipment as outlined in this instruction manual. The following symbols are used to set off text in this instruction manual.
- Page 7 U-LH100HGAPO (x 6) U-LH100HG UV cut plate ND filter/exciter balancer insertion slot (Page 27) 6-position filter insertion slot (Page 23) Motorized Reflected Fluorescence Illuminator BX-RFAA Aperture iris diaphragm knob (Page 12) Field iris diaphragm knob (Page 11) Motorized Revolving Nosepiece...
- Page 8 }The BX-RFAA can accept up to six fluorescence mirror units. Fluorescence Mirror Unit # Every mirror unit is composed of a dichroic mirror, barrier filter and 18 models including the U-MWU2 excitation filter which are suitable for each excitation method. Basi- cally, do not disassemble the mirror unit.
- Page 9 REFLECTED FLUORESCENCE OBSERVATION PROCEDURE }For the operating procedures for simultaneous observations of phase contrast or transmitted Nomarski DIC observation with fluorescence observation, see chapter 4, “SIMULTANEOUS FLUORESCENCE OBSERVATION METHODS” (page 15). (Controls Used) (Page) Preparation · Attach the fluorescence mirror units and objectives which are suitable for the observation method to be used. (P.
- Page 10 ‰ Š Mercury Lamp Housing U-LH100HGAPO U-LH100HG Motorized Reflected Fluorescence Illuminator BX-RFAA Motorized Revolving Nosepiece Œ ‹ Power Supply Unit (for 100 W mercury burner) ² Microscope ³ BX61 BX62, etc. † … Control Box BX-UCB Hand Switch U-HSTR2 ƒ...
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Page 11: Using The Controls
U-MBFL3 mirror unit equipped with a built-in ND filter or add a 3% ND filter to the U-MBF3. }Use of fluorescence mirror units according to the excitation wavelength Olympus has prepared some sets of fluorescence mirror unit combined with appropriate filters which are variable de- pending on wavelengths. - Page 12 Dichroic Mirrors and Filter Configurations of Fluorescence Mirror Units Excitation Mirror Unit Dichroic Mirror Excitation Filter Barrier Filter Fluorochrome Method · Autofluorescence observation U-MWU2 BP330-385 · DAPI staining: DNA DM400 BA420 · Hoechest 33258, 33342 staining: Chro- U-MNU2 BP360-370 mosome ·...
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Page 13: Objectives For Various Observation Modes
Objectives for Various Observation Modes Reflected Light Fluorescence Phase Contrast Transmitted Light Objective Difference Nomarski DIC U, V, BV B, IB, G, IY UPlanApo ¦ ¦ – – ¦ ¦ **¦** ¦ 10XO ¦ ¦ – ¦ 10XW ¦ ¦ –... -
Page 14: Centering The Field Iris Diaphragm
Centering the Field Iris Diaphragm (Fig. 1) 1. Engage the shutter in the light path by pressing the RSHT button on the hand switch. 2. Engage the B or IB mirror unit in the light path by pressing the MU+ or MU–... -
Page 15: Centering The Aperture Iris Diaphragm
Centering the Aperture Iris Diaphragm (Fig. 2) 1. Engage the shutter in the light path by pressing the RSHT button on the hand switch. 2. Engage the B or IB mirror unit in the light path by pressing the MU+ or MU–... -
Page 16: Centering The Mercury Burner
Centering the Mercury Burner (Fig. 3) @ ² ³ }Set the main switch to “ I ” (ON) and wait for 5 to 10 minutes until the arc image stabilizes before proceeding to the mercury burner centering. 1. Engage the shutter in the light path by pressing the RSHT button on the hand switch. -
Page 17: Using The Nd Filters
Precise Centering of Mirror Arc Image }The mirror arc image position has been adjusted and fixed at the factory. ƒ Perform the centering of the mirror arc image after completing the center- ing of the mercury burner and only when you want to make your adjust- ments very strict and precise. -
Page 18: Simultaneous Fluorescence Observation Methods
SIMULTANEOUS FLUORESCENCE OBSERVATION METHODS }By properly combining equipment, this system can be used in transmitted light brightfield observation, transmitted phase contrast observation and transmitted light DIC observation in addition to the reflected fluorescence observation. With speci- mens that fade rapidly, fading can be minimized by initially using transmitted light phase contrast or transmitted DIC observation for positioning. -
Page 19: Troubleshooting Guide
Under certain conditions, performance of the system may be adversely affected by factors other than defects. If problems occur, please review the following list and take remedial action as needed. If you cannot solve the problem after checking the entire list, please contact your local Olympus representative for assistance. Problem... - Page 20 Problem Cause Remedy Page c) Mercury burner flickers or is dark. It is soon after ignition. Leave for 10 minutes or more after ig- nition. Burner life has expired. If hour counter indicates 200 hours (USH102D) or 300 hours (HBO103W/2), replace it.
- Page 21 SPECTRAL CHARACTERISTICS OF FILTERS 1. U-excitation (Wide band) 4. BV-excitation (Wide band) U-MWU2 U-MWBV2 Wavelength nm 2. U-excitation (Narrow band) 5. BV-excitation (Narrow band) U-MNU2 U-MNBV2 Wavelength nm 3. V-excitation (Narrow band) 6. B-excitation (Wide band) U-MNV2 U-MWB2 Wavelength nm...
- Page 22 7. B-excitation (Narrow band) 10. IB-excitation (Narrow band) U-MNB2 U-MNIB2 Wavelength nm 8. B-excitation (Superwide band) 11. G-excitation (Wide band) U-MSWB2 U-MWG2 Wavelength nm 9. IB-excitation (Wide band) 12. G-excitation (Narrow band) U-MWIB2 U-MNG2...
- Page 23 13. G-excitation (Superwide band) 16. U-excitation for color separation (Narrow band) U-MSWG2 U-MNUA2 14. IG-excitation (Wide band) 17. IB-excitation for color separation (Wide band) U-MWIG2 U-MWIBA2 15. IV-excitation (Wide band) 18. IB-excitation for color separation (Narrow band) U-MNIBA2 U-MWIY2...
- Page 24 Typical Example of Emission Spectrum of Ultrahigh-Vacuum Mercury Burner For fluorochrome emission, a light beam having a specific wavelength is selected from a wide spectrum of wavelengths. The five major peaks of luminance are at wavelengths of 365/366, 404.7, 435, 546.1 and 577.0/579.1 nm. In addition, light beams having wave- lengths of 334.2 and 490 nm (at rather low luminance) are also applicable to fluoro- chrome emission.
- Page 25 · UIS (Universal Infinity System) optics Fluorescence Illuminator · Magnification: 1X (Superwide field compatible: FN 26.5) BX-RFAA · Observation switching: Motorized mirror unit turret (Switching rate approx. 0.8 sec.) Mirror unit turret carrying max. 6 mirror units. · Aperture and field iris diaphragms: both centrable and detachable ·...
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Page 26: Optional Modules
OPTIONAL MODULES # The sliding performance of the U-RSL6 or U-RSL6EM filter slider may drop when it has been used for 2000 or more times of reciprocation. In this case, remove the dirt and contamination on the sliding surface. If it is expected to use the slider for more than 2000 times of reciprocation, apply a thin layer of lubricant such as grease on the sliding surface. - Page 27 Using the Joint Plates The joint plate @ can be attached and locked between the slider knob and slider as shown in Fig. 6. The joint plates should be attached on both ends of the filter slider. By locking with the joint plates, you can switch the barrier and excitation filters together as a set.
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Page 28: 6-Position Barrier Filter Slider U-Rsl6Em
6-Position Barrier Filter Slider U-RSL6EM }This filter slider is for common use with the BX-URA2 or BX-RFA illuminator and accommodates a total of six barrier filters. Slider knob Joint plate Light shield plate (x 4) Inscription sticker position Diameter 8 mm, depth 1 mm Light shied position Barrier filter insertion positions (x 6) Diameter 25 mm, thickness max. -
Page 29: Rectangular Field Stop Unit Bx-Rfss (For Exclusive Use With The Bx-Rfa/Rfaa)
}Rectangle area: A rectangle which circumscribes the field with a number of 22 (the center of the rectangle should be located at the center of field). The rectangle iris diaphragm cannot be rotated. NOTES The BX-RFAA illuminator cannot be attached or removed while the BX-RFSS is installed. If you want to install the BX-RFAA illuminator, remove the BX-RFSS temporarily. -
Page 30: Exciter Balancers U-Exbabg/Exbaub/Exbaug (For Exclusive Use With The Bx-Rfa/Rfaa)
* To use an exciter balancer with the BX-RFAA, remove the adjustment lever and attach it to the opposite end. Clamping screw Adjustment lever* Installation Procedure (Fig. - Page 31 3. Push in the adjustment lever of the balancer slider to be used to engage the filter in the light path. }The angle of each adjustment lever can be adjusted in the range shown below, only when the lever is pushed in. Front (Observer) U-EXBAUG U-EXBABG or U-EXBAUB...
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Page 32: Assembly
The module numbers shown in the following diagram are merely the typical examples. For the modules with which the module numbers are not given, please consult your Olympus representative or the catalogues. # When assembling the microscope, make sure that all parts are free of dust and dirt, and avoid scratching any parts or touching glass surfaces. -
Page 33: 9-2 Detailed Assembly Procedures
9-2 Detailed Assembly Procedures Attaching the Revolving Nosepiece (Motorized Revolving Nosepiece) }With certain series of motorized revolving nosepiece products, the connector of the BX-RFAA illuminator should be unlocked before proceeding to the attaching operation as described below. U-D6REM/5BDREM These revolving nosepieces can be installed in the same way as manual revolving nosepieces. - Page 34 Attaching the Fluorescence Mirror Units (Figs. 11 & 12) 1. Using the Allen screwdriver, loosen the clamping screw @ at the right side of the illuminator. 2. Pull out the turret and place it upside down. }Dummy mirror units ² are mounted in the mirror unit positions. Remove the dummy mirror units from the positions you want to mount mirror units by loosening the clamping screw ³...
- Page 35 Making an Optional Fluorescence Mirror Unit }You can also fabricate optional fluorescence mirror units by fitting a commercially available barrier filter, excitation filter or dichroic mirror in the U-MF2 mirror unit frame. Dimensions of Optical Parts · Barrier filter Diameter 25 -0.1/-0.2 mm, max. thickness 6 mm ·...
- Page 36 Attaching the Mercury Burner (Figs. 13 - 16) 1. Loosen the socket clamping screw @ using the provided Allen screw- driver. 2. Hold the upper section of lamp housing and pull it upward to remove the ² socket section. # To prevent malfunctions, do not hold the lamp housing by the cen- tering knobs ².
- Page 37 Resetting the Burner Hour Counter 1. Press the center section @ of the reset button ² on the power supply unit’s front panel to reset the hour counter to “000.0”. }The hour counter shows elapsed time in hours. The service life of a burner is 200 (USH102D) or 300 (HBO103W/2) hours.
- Page 38 Make sure that the main switch is set to “ ” (OFF) before connecting the power cord. ² Always use the power cord provided by Olympus. If no power cord is provided with the microscope, please select the proper power ³...
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Page 39: I Proper Selection Of The Power Supply Cord
If no power supply cord is provided, please select the proper power supply cord for the equipment by referring to “ Specifications ” and “ Certified Cord ” below: CAUTION: In case you use a non-approved power supply cord for Olympus products, Olympus can no longer warrant the electrical safety of the equipment. - Page 40 Table 2 HAR Flexible Cord APPROVAL ORGANIZATIONS AND CORDAGE HARMONIZATION MARKING METHODS Alternative Marking Utilizing Printed or embossed Harmoniza- Black-Red-Yellow Thread (Length tion Marking (May be located on Approval Organization of color section in mm) jacket or insulation of internal wir- ing) Black Yellow...
- Page 41 MEMO...
- Page 42 2-43-2,Hatagaya, Shibuya-ku, Tokyo, Japan Postfach 10 49 08, 20034, Hamburg, Germany 2 Corporate Center Drive, Melville, NY 11747-3157, U.S.A. 491B River Valley Road, #12-01/04 Valley Point Office Tower, Singapore 248373 2-8 Honduras Street, London EC1Y OTX, United Kingdom. 104 Ferntree Gully Road, Oakleigh, Victoria, 3166, Australia This publication is printed on recycled paper.