Reflected Light Brightfield/Darkfield Simultaneous Observation - Olympus GX53 Instructions Manual

4-3 Reflected light brightfield/darkfield simultaneous observation

Select the brightfield (BF) observation.
Engage the 10X objective in the light path.
Engage the objective with the magnification to be used
in the light path and bring the sample into focus.
Adjust the aperture diaphragm and the field diaphragm.
(Fully open the aperture diaphragm during darkfield observation.)
Set the MIX slider for reflected light observation.
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Set the main switch to (ON).
Remove the analyzer, polarizer or
filter sliders from the light path.
Select the observation light path.
Place a sample on the stage.
Bring the sample into focus.
Adjust the brightness.
Adjust the interpupillary distance.
Adjust the diopter.
Adjust the brightness.
Observation
Operation part
Main switch
BF/DF selection lever
Eyepiece/camera light path selection
knob
Light path selection knob on trinocular tube
or Observation/SP selection knob on the
side port
Stage plate
X-axis/Y-axis knobs
Revolving nosepiece
Coarse/Fine focusing knobs
Brightness control knob
Interpupillary distance adjustment scale
Diopter adjustment ring
Revolving nosepiece
Coarse/Fine focusing knobs
Aperture diaphragm lever
Field diaphragm lever
Brightness control knob
MIX slider for reflected light observation
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