Plumbing The Detector - Waters ACQUITY UPLC Operator's, Overview And Maintenance Manual

Plumbing the detector

Warning:
damage the instrument. See
more information.
Plumbing the detector involves connecting the flow cell and installing a
backpressure regulator, if necessary.
Although the in-line degasser removes most of the gas (air) from solvents,
some gas is reintroduced during partial loop injections. Under pressure, this
gas remains in solution. However, because the post-column pressure is
normally much lower than the pre-column pressure, any dissolved gas in the
post-column affluent comes out of solution and produces an unstable baseline
characterized by large, unexpected spikes.
A backpressure regulator maintains a minimum post-column pressure of
1724 kPa (17 bar, 250 psi), eliminating post-column outgassing and ensuring
a smooth baseline.
Requirement:
system, the backpressure regulator is required for optimum performance.
Note, however, that if a mass spectrometer or other detector is connected
downstream of the detector, you need not install a backpressure regulator.
The length of the tubing connecting to the mass spectrometer or other detector
helps to maintain the backpressure on the flow cell.
Recommendation:
any columns you are connecting to the detector before connecting them.
ACQUITY UPLC System Documentation CD or ACQUITY UPLC
See also:
H-Class System Documentation CD.
Using incompatible solvents can injure you and severely
If the ACQUITY PDA/eλPDA detector is the last detector in the
To avoid particulate contamination in the flow cell, flush
"Solvent considerations" on page 98
Plumbing the detector
for
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