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Waters TQ Detector Quick Start Manual

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Waters TQ Detector
Quick Start Guide
71500126803/Revision A
Copyright © Waters Corporation 2007.
All rights reserved.

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  • Page 1 Waters TQ Detector Quick Start Guide 71500126803/Revision A Copyright © Waters Corporation 2007. All rights reserved.
  • Page 2 Corporation assumes no responsibility for any errors that may appear in this document. This document is believed to be complete and accurate at the time of publication. In no event shall Waters Corporation be liable for incidental or consequential damages in connection with, or arising from, its use.
  • Page 3 Customer comments Waters’ Technical Communications department invites you to tell us of any errors you encounter in this document or to suggest ideas for otherwise improving it. Please help us better understand what you expect from our documentation so that we can continuously improve its accuracy and usability.

  • Page 4: Safety Considerations

    Instrument-specific safety considerations Solvent leakage hazard The source exhaust system is designed to be robust and leak-tight. Waters recommends you perform a hazard analysis, assuming a maximum leak into the laboratory atmosphere of 10% HPLC eluate. Warning: •...

  • Page 5: Flammable Solvents Hazard

    LC solvent if the nitrogen supply fails. High temperature hazard Warning: The source enclosure can be hot. To avoid burn injuries, avoid touching the source enclosure when operating or servicing the TQ Detector. Waters TQ Detector high temperature hazard: Source enclosure assembly...

  • Page 6: High Voltage Hazard

    High voltage hazard Warning: • To avoid electric shock, do not remove the TQ Detector’s protective panels. The components they cover are not user-serviceable. • To avoid non-lethal electric shock, any equipment connected to the ESI and IonSABRE™ APCI probes must be grounded.

  • Page 7: Operating This Device

    For in vitro diagnostic use. Intended use The Waters Tandem Quadrupole (TQ) Detector is intended to be used as a research tool to deliver authenticated mass measurement in both MS and MS/MS modes. The Waters TQ Detector can be used for general In Vitro Diagnostic applications, only by professionally trained and qualified laboratory personnel.

  • Page 8: Calibration

    When analyzing samples from a complex matrix such as soil, tissue, serum/plasma, whole blood, etc., note that the matrix components can adversely affect LC/MS results, enhancing or suppressing ionization. To minimize these matrix effects, Waters recommends you adopt the following measures: •...

  • Page 9: Ivd Authorized Representative Information

    IVD authorized representative information IVD authorized representative Waters Corporation (Micromass UK Limited) is registered in the United Kingdom with the Medicines and Healthcare Products Regulatory Agency (MHRA) at market Towers, 1 Nine Elms Lane, London, SW8 5NQ. The reference number is IVD000167.

  • Page 11: Table Of Contents

    Calibration ....................... viii Quality control ....................viii IVD authorized representative information ..........ix IVD authorized representative ................ix 1 Waters TQ Detector Overview ............1-1 Overview ......................1-2 Waters TQ Detector ..................1-2 ACQUITY TQD UPLC/MS system ..............1-4 MassLynx mass spectrometry software and data system ......1-5 ACQUITY UPLC Console................
  • Page 12 Installing the solvent manifold drip tray ............2-9 Installing the reservoir bottles..............2-10 Diverter valve positions................. 2-11 Purging the infusion syringe................. 2-13 Rebooting the TQ Detector ................2-14 Rebooting the TQ Detector by pressing the reset button ......2-14 Table of Contents...
  • Page 13 Shutting down the TQ Detector ..............2-15 Putting the TQ Detector in Standby mode for overnight shutdown ... 2-15 Complete TQ Detector shutdown ..............2-16 Emergency TQ Detector shutdown ............... 2-17 3 ESI and ESCi Modes of Operation ............. 3-1 Introduction .......................
  • Page 14 Caution symbol ....................A-5 Warnings that apply to all Waters instruments ......... A-5 Electrical and handling symbols ..............A-13 Electrical symbols ..................A-13 Handling symbols ..................A-14 Table of Contents...

  • Page 15: Waters Tq Detector Overview

    Waters TQ Detector Overview This chapter describes the instrument, including its controls and gas and plumbing connections. Contents: Topic Page Overview Ionization techniques and source probes Ion optics MS operating modes 1-10 MS/MS operating modes 1-11 Sample inlet 1-14 Vacuum system...

  • Page 16: Overview

    (APCI) and electrospray ionization (ESI). Optional ionization modes are IonSABRE™ APCI and APPI (atmospheric pressure photoionization). For TQ Detector specifications, see the Waters TQ Detector Site Preparation Guide. Waters TQ Detector: TP02592 Waters TQ Detector Overview...
  • Page 17 IntelliStart enables simplified set-up of the system for use in routine analytical and open access applications. The IntelliStart fluidics system is built into the TQ Detector. It delivers sample directly to the MS probe from the LC column or from two integral reservoirs.

  • Page 18: Acquity Tqd Uplc/Ms System

    ACQUITY TQD UPLC/MS system The Waters TQ Detector is designed for compatibility with the Note: ACQUITY UPLC system; if you are not using an ACQUITY UPLC system, refer to the documentation relevant to the LC system being used. The ACQUITY TQD UPLC/MS system includes an ACQUITY UPLC system and the Waters TQ Detector.

  • Page 19: Masslynx Mass Spectrometry Software And Data System

    • Configuring the instrument. • Creating LC inlet and MS/MS methods that define operating parameters for a run. • Using IntelliStart software to tune and mass calibrate the TQ Detector. • Running samples. • Monitoring the run. • Acquiring data.

  • Page 20: Acquity Uplc Console

    The ACQUITY UPLC Console functions independently of MassLynx and does not recognize or control the data system. See also: ACQUITY UPLC System console online Help for details of the TQ Detector. TQ Detector ACQUITY UPLC Console page: Waters TQ Detector Overview...

  • Page 21: Ionization Techniques And Source Probes

    (ESCi) Combined electrospray ionization and atmospheric pressure chemical ionization (ESCi) is supplied as standard equipment on the TQ Detector. In ESCi, the standard ESI probe is used in conjunction with a corona pin to allow alternating acquisition of ESI and APCI ionization data, facilitating high throughput and wider compound coverage.

  • Page 22: Atmospheric Pressure Chemical Ionization

    A dedicated high performance atmospheric pressure chemical ionization (APCI) probe is offered as an option. See also: The Waters TQ Detector Operator’s Guide for full details. Atmospheric pressure photoionization Atmospheric pressure photoionization (APPI) is offered as an option. It uses photons generated by a krypton-discharge ultra-violet (UV) lamp (∼10.2 eV) to...

  • Page 23: Ion Optics

    Ion optics The TQ Detector’s ion optics operate as follows: • Samples from the LC or Intellistart fluidics system are introduced at atmospheric pressure into the ionization source. • The ions pass through the sample cone into the vacuum system.

  • Page 24: Ms Operating Modes

    MS/MS analysis and for fault diagnosis. The selected ion recording (SIR) mode of operation is used as a quantitation mode when no suitable fragment ion can be found to perform a more specific multiple reaction monitoring (MRM) analysis. 1-10 Waters TQ Detector Overview...

  • Page 25: Ms/Ms Operating Modes

    MS/MS operating modes The following table shows the MS/MS operating modes. MS/MS operating modes : Operating mode Collision cell Product Static (at Pass all masses Scanning (daughter) ion precursor mass) spectrum Precursor Scanning Static (at product (parent) ion mass) spectrum Static (at Static (at product precursor mass)

  • Page 26: Precursor (Parent) Ion Mode

    MS2 are static, greater dwell time on the ions of interest is allowed, and therefore better sensitivity compared to scanning MS/MS. This is the most commonly used acquisition mode for quantitative analysis, allowing the compound of interest to be isolated from the chemical background noise. 1-12 Waters TQ Detector Overview...

  • Page 27: Constant Neutral Loss Mode

    Multiple reaction monitoring mode: Collision cell Static (at precursor mass) Pass all masses Static (at product mass) Typical application You typically use MRM to quantify known analytes in complex samples: • Drug metabolite and pharmacokinetic studies. • Environmental, for example, pesticide and herbicide analysis. •...

  • Page 28: Sample Inlet

    Pirani gauge. The gauge also serves as a switch, discontinuing operation when it senses vacuum loss. A vacuum isolation valve isolates the source from the mass analyzer, allowing routine source maintenance without venting. 1-14 Waters TQ Detector Overview...

  • Page 29: Rear Panel

    Rear panel The following figure shows the rear panel locations of the connectors used to operate the TQ Detector with external devices. TQ Detector rear panel: Analog Inject Start Ground Ground Not Used Event Stop Flow Switch 3 Ground Ground...

  • Page 30: Intellistart Fluidics System Overview

    IntelliStart fluidics system overview The IntelliStart fluidics system is built into the TQ Detector. The system delivers sample directly to the MS probe in one of two ways: • From the LC column • From two integral reservoirs. Tip: The integral reservoirs can also deliver sample through direct or combined infusion to enable optimization at analytical flow rates.

  • Page 31: Intellistart Fluidics System Operation

    IntelliStart to perform. See also: The TQ Detector online Help for further details of IntelliStart. Operating the IntelliStart fluidics system from the ACQUITY UPLC Console To operate the IntelliStart fluidics system from the Instrument Console: In the MassLynx window, click MS Console.

  • Page 32: Operating The Intellistart Fluidics System From The Tune Window

    To operate the IntelliStart fluidics system from the Tune window: In the MassLynx window, click MS Tune. In the TQ Detector Tune window, click the Fluidics page. Set the Flow Control parameters according to the instructions in the MassLynx online Help.
  • Page 33 To program the MS method: In the MassLynx window, click MS Method. In the MS Methods window, click Options > Method Events. In the Method events dialog box, select the appropriate flow state, as shown in the following table. Flow states : State Syringe TQ probe...
  • Page 34 1-20 Waters TQ Detector Overview...

  • Page 35: Preparing The Waters Tq Detector For Operation

    Preparing the Waters TQ Detector for Operation This chapter describes how to start and shut-down the TQ Detector. Contents: Topic Page Starting the TQ Detector Preparing the IntelliStart fluidics system Rebooting the TQ Detector 2-14 Shutting down the TQ Detector...

  • Page 36: Starting The Tq Detector

    Using incompatible solvents can cause severe damage to the Caution: instrument. • Refer to Appendix C of the Waters TQ Detector Operator’s Guide for TQ Detector solvent information. • Refer to Appendix C of the ACQUITY UPLC System Operator’s Guide for more information on solvent compatibility with ACQUITY.
  • Page 37 Press the power switch on the top, left-hand side of the TQ Detector and ACQUITY instruments. Each system instrument “beeps” and runs a series of startup tests. Allow 3 minutes for the embedded PC to initialize. An audible alert sounds when the PC is ready.
  • Page 38 Click Control > Pump to start the roughing pump. The operate LED remains off. There is a 20-second delay, during which the turbopump is starting, Tip: before the roughing pump starts. IntelliStart displays “Instrument in standby”. Preparing the Waters TQ Detector for Operation...
  • Page 39 When the TQ Detector is in good operating condition, IntelliStart displays “Ready”. Tip: Clicking Resolve should prepare the system for operation, putting the TQ Detector into Operate mode. If clicking Resolve fails to put the instrument into Operate mode, IntelliStart displays corrective actions. Starting the TQ Detector...

  • Page 40: Configuring Intellistart

    Properties. Click Apply > OK. Verifying the instrument’s state of readiness When the TQ Detector is in good operating condition, the power LED shows constant green and the operate LED is off. You can view any error messages in IntelliStart.

  • Page 41: Running The Tq Detector At High Flow Rates

    Running the TQ Detector at high flow rates ACQUITY UPLC is run at high flow rates. To optimize desolvation, and thus sensitivity, the ACQUITY TQD system should be run at appropriate gas flows and desolvation temperatures. IntelliStart automatically sets these when you enter a flow rate, according to the following table.

  • Page 42: Monitoring The Tq Detector Leds

    Monitoring the TQ Detector LEDs Light-emitting diodes on the TQ Detector indicate its operational status. Power LED The power LED, to the top, left-hand side of the TQ Detector’s front panel, indicates when the TQ Detector is powered-on or powered-off. Operate LED The operate LED, to the right of the power LED, indicates the operating condition.

  • Page 43: Preparing The Intellistart Fluidics System

    Preparing the IntelliStart fluidics system See also: Appendix B of the Waters TQ Detector Operator’s Guide. Installing the solvent manifold drip tray Required materials Chemical-resistant, powder-free gloves To install the solvent manifold drip tray: Warning: The solvent manifold drip tray can be contaminated with biohazardous and/or toxic materials.

  • Page 44: Installing The Reservoir Bottles

    The reservoir bottles can be contaminated with Warning: biohazardous and/or toxic materials. Always wear chemical-resistant, powder-free gloves while performing this procedure. Remove the reservoir bottle caps. Screw the reservoir bottles onto the TQ Detector as shown below. TP02630 2-10 Preparing the Waters TQ Detector for Operation...

  • Page 45: Diverter Valve Positions

    Diverter valve positions Column and syringe in home position after power-up After power-up, the flow path between the column and waste is open. The syringe is empty, and the flow path between it and waste is open. Probe Waste Idle Column Syringe Waste...
  • Page 46 Infusion position in infusion mode Probe Infusion Infusion Column Syringe Waste Reservoir A Reservoir B Combined position with LC flow and syringe in idle mode Probe Combined Idle Column Syringe Waste Reservoir A Reservoir B 2-12 Preparing the Waters TQ Detector for Operation...

  • Page 47: Purging The Infusion Syringe

    Depending on the solutions used, the IntelliStart fluidics system can Tip: require more then one purge cycle to minimize carryover. To purge the infusion syringe: In the ACQUITY UPLC Console system tree, expand TQ Detector. Click Interactive Display. Select the required solution reservoir. Click to purge the system.

  • Page 48: Rebooting The Tq Detector

    The reset button shuts down the electronics momentarily and causes the TQ Detector to reboot. To reboot the TQ Detector by pressing the reset button: Open the TQ Detector’s front left door. Press the red reset button on the top, left-hand side of the instrument.

  • Page 49: Shutting Down The Tq Detector

    Shutting down the TQ Detector Recommendation: You should always leave the TQ Detector in Operate mode except when performing routine maintenance. It is not necessary to switch to Standby mode. However, shutting down the TQ Detector is acceptable provided that you consider warm-up time on restarting. If you do shut down the TQ Detector, refer to the instructions in this section.

  • Page 50: Complete Tq Detector Shutdown

    To isolate the instrument, you must disconnect the power cable from the back of the instrument. Disconnect the power cable from the back of the TQ Detector. Power-off all other instruments and the workstation. Note: The fans inside some instruments run continuously, even after you power-off the instruments.

  • Page 51: Emergency Tq Detector Shutdown

    Caution: Data can be lost during an emergency shutdown. Operate the power button on the front of the TQ Detector. Disconnect the power cable from the back of the TQ Detector. Shutting down the TQ Detector...
  • Page 52 2-18 Preparing the Waters TQ Detector for Operation...

  • Page 53: Esi And Esci Modes Of Operation

    • ESI (electrospray ionization) • ESCi (combined electrospay and atmospheric pressure chemical ionization) If your system uses APCI mode, refer to Chapter 6 of the Waters TQ Detector Operator’s Guide. Contents: Topic Page Introduction Installing the ESI probe...

  • Page 54: Introduction

    To avoid electric shock, ensure that the instrument is suitably prepared before commencing this procedure. Prepare the instrument for working on the source. Chapter 5 of the Waters TQ Detector Operator’s Guide. See also: Warning: The source can be hot. To avoid burn injuries, take great care while working with the instrument’s access door open.
  • Page 55 Warning: The ESI probe tip is sharp. To avoid puncture wounds, handle the ESI probe with care. Remove the protective sleeve, if fitted, from the ESI probe tip. Ensure that the contacts on the ESI probe align with the probe adjuster assembly contacts, and carefully slide the ESI probe into the hole in the probe adjuster assembly.
  • Page 56 ESI probe mounted on the source enclosure, showing the connections to the front panel: Vernier probe adjuster Thumbscrew Nebulizer gas connection Diverter valve Desolvation gas connection ESI probe electrical lead Probe adjuster assembly electrical lead Probe adjuster assembly ESI probe Connect the ESI probe’s PTFE tube to the nebulizer gas connection.

  • Page 57: Installing The Corona Pin

    Installing the corona pin Required materials • Chemical-resistant, powder-free gloves • Needle-nose pliers • 80:20 acetonitrile/water To install the corona pin: Warning: The ACQUITY UPLC system connections, ESI probe, and source can be contaminated with biohazardous and/or toxic materials. Always wear chemical-resistant, powder-free gloves while performing this procedure.
  • Page 58 Use the needle-nose pliers to remove the blanking plug from the corona pin mounting contact. Store the blanking plug in a safe location. Tip: Corona pin mounting contact: Corona pin mounting contact blanking plug TP02660 The corona pin tip is sharp. To avoid puncture wounds, Warning: handle the corona pin with care.

  • Page 59: Optimizing The Esi Probe For Esci Operation

    Corona pin: ESI probe tip Corona pin TP02695 Sample cone tip Use the vernier probe adjuster to position the ESI probe tip so that it is pointing approximately mid-way between the tips of the sample cone and corona pin. See also: Figure titled “ESI probe mounted on the source enclosure, showing the connections to the front panel”...
  • Page 60 Click OK. In the MassLynx Tune window, click Ion Mode > ESCi+. Select box numbers 1 and 2, clear box numbers 3 and 4 (above the peak display). In row 1, set Ion Mode to ES. In row 2, set Ion Mode to APcI. In each row, set Mass to 42 and Span to 5.

  • Page 61: Removing The Corona Pin

    Removing the corona pin Required materials • Chemical-resistant, powder-free gloves • Needle-nose pliers To remove the corona pin: The ACQUITY UPLC system connections, corona pin, Warning: ESI probe, and source can be contaminated with biohazardous and/or toxic materials. Always wear chemical-resistant, powder-free gloves while performing this procedure.
  • Page 62 The corona pin tip is sharp. To avoid puncture wounds, Warning: handle the corona pin with care. Caution: To avoid damaging to the corona pin’s tip and bending the pin, use the needle nose pliers to grip the corona pin at the end that fits into the mounting contact.

  • Page 63: Removing The Esi Probe

    To avoid electric shock, ensure that the instrument is suitably prepared before commencing this procedure. Prepare the instrument for working on the source. Chapter 5 of the Waters TQ Detector Operator’s Guide. See also: Warning: The ESI probe and source can be hot. To avoid burn injuries, take great care while working with the instrument’s...
  • Page 64 If available, fit the protective sleeve to the ESI probe tip. Close the instrument’s access door. 3-12 ESI and ESCi Modes of Operation...

  • Page 65: Operating The Waters Tq Detector

    Operating the Waters TQ Detector This chapter is an introduction to operating your TQ Detector; it explains these tasks: • Setting-up your TQ Detector. • Performing a sample tune. • Developing instrument methods. • Verifying the system. Contents: Topic Page...

  • Page 66: Setting-Up The Instrument

    In the following example, sodium cesium iodide is used as the calibrant solution and sulfadimethoxine the tune sample. You can substitute solutions suitable for your requirements. Tip: See also: The TQ Detector online Help for further details of IntelliStart. Required materials • Sodium cesium iodide solution (2 ng/µL) •...
  • Page 67 14. When the purge finishes, click to purge the system again. To specify the instrument set-up parameters: In the ACQUITY UPLC Console system tree, click TQ Detector. Ensure that Ion Mode is ES+. In the ACQUITY UPLC Console system tree, click IntelliStart.
  • Page 68 You can use alternative reference solutions to calibrate at higher masses. The tune and calibration results are written to the files in the Instrument Tune and Calibration text boxes; you can use the default files, enter new names, or Browse for files. Operating the Waters TQ Detector...
  • Page 69 To specify the sample tune parameters: In the IntelliStart Setup Parameters dialog box, click the Sample Tune tab. Click Copy Instrument Setup masses. Rationale: The Tune Masses from the Instrument Setup tab are copied into the Sample Tune Masses boxes. Instrument Setup Parameters dialog box Sample Tune tab: In the Tune text box, enter sulfadimethoxine.ipr.
  • Page 70 LC flow are set correctly. Click OK. Instrument set-up starts. An autotune on the calibrant is Result: followed by automatic calibration. The ACQUITY UPLC Console displays the progress of the setup. Operating the Waters TQ Detector...

  • Page 71: Performing A Sample Tune

    Example ACQUITY UPLC Console display during calibration: IntelliStart creates tune and calibration files, which are saved as specified on the Instrument Setup Parameters dialog box’s Instrument Setup tab. Once calibration is complete, the sample tune starts on the four masses defined in the IntelliStart Setup Parameters dialog box. When the sample tune is complete, the results are written to the Sulfadimethoxine.ipr file.
  • Page 72 In the ACQUITY UPLC Console system tree, click TQ Detector. Ensure that the Ion Mode is ES+. In the ACQUITY UPLC Console system tree, click IntelliStart. Clear the Instrument Setup check box. If you require system pre-checking, click Pre-checks. Rationale: If Pre-checks is selected, when IntelliStart starts the sample tune, it determines whether the existing tune is still valid.

  • Page 73: Developing Experiment Methods

    See also: “To prepare the IntelliStart fluidics system:” on page 4-2. In the ACQUITY UPLC Console system tree, click TQ Detector. Ensure that the Ion Mode is ES+. In the ACQUITY UPLC Console system tree, click IntelliStart. Clear the Instrument Setup check box.
  • Page 74 Sulfadimethoxine.exp file. The Validation pane selection saves optimization data for validation purposes, and creates an autotune report file (Sulfadimethoxine.xml). A green check mark indicates a successful run; a red cross indicates a failure. 4-10 Operating the Waters TQ Detector...

  • Page 75: Verifying The System Using System Qc

    Verifying the system using System QC The System QC function uses pre-defined LC/MS methods to test the system by injecting a diagnostic sample on-column. The sample helps identify any problems with the configured system by providing these data: • Sensitivity (signal-to-noise ratio) •...
  • Page 76 Aqudb folder of the System QC project before performing the verification. To perform a manual verification using System QC: In the ACQUITY UPLC Console, click System QC. Click Start Instrument Setup Parameters dialog box System QC tab with default parameters: 4-12 Operating the Waters TQ Detector...
  • Page 77 In the IntelliStart Setup Parameters dialog box’s System QC tab, enter the number of pre-run and run injections, the vial (that is, the position of your sample in the Autosampler) and the injection volume. At least two injections are required for a run, because some of the Tip: validation parameters use standard deviation.
  • Page 78 Click Start Result: A message appears reminding you to ensure that the sample vial, QC parameters and LC flow are set correctly. Click OK. Result: System QC starts. The ACQUITY UPLC Console displays its progress. 4-14 Operating the Waters TQ Detector...
  • Page 79 Safety Advisories Waters instruments display hazard symbols designed to alert you to the hidden dangers of operating and maintaining the instruments. Their corresponding user guides also include the hazard symbols, with accompanying text statements describing the hazards and telling you how to avoid them.

  • Page 80: A Safety Advisories

    Heed all warnings when you install, repair, and operate Waters instruments. Waters assumes no liability for the failure of those who install, repair, or operate its instruments to comply with any safety precaution.

  • Page 81: Warnings That Apply To Particular Instruments, Instrument Components, And Sample Types

    The following warnings can appear in the user manuals of particular instruments and on labels affixed to them or their component parts. Burst warning This warning applies to Waters instruments fitted with nonmetallic tubing. Pressurized nonmetallic, or polymer, tubing can burst. Warning: Observe these precautions when working around such tubing: •...
  • Page 82 Standby mode before touching areas marked with this high voltage warning symbol. Biohazard warning This warning applies to Waters instruments that can be used to process material that might contain biohazards: substances that contain biological agents capable of producing harmful effects in humans.

  • Page 83: Caution Symbol

    Chemical hazard warning This warning applies to Waters instruments that can process corrosive, toxic, flammable, or other types of hazardous material. Warning: Waters instruments can be used to analyze or process potentially hazardous substances. To avoid injury with any of these materials, familiarize yourself with the materials and their hazards, observe Good Laboratory Practices (GLP), and consult your organization’s safety...
  • Page 84 Attention: Changes or modifications to this unit not expressly approved by the party responsible for compliance could void the user’s authority to operate the equipment. Important: Toute modification sur cette unité n’ayant pas été expressément approuvée par l’autorité responsable de la conformité à la réglementation peut annuler le droit de l’utilisateur à...
  • Page 85 • Keine Schläuche verwenden, die stark geknickt oder überbeansprucht sind. • Nichtmetallische Schläuche nicht für Tetrahydrofuran (THF) oder konzentrierte Salpeter- oder Schwefelsäure verwenden. • Durch Methylenchlorid und Dimethylsulfoxid können nichtmetallische Schläuche quellen; dadurch wird der Berstdruck des Schlauches erheblich reduziert. Warnings that apply to all Waters instruments...
  • Page 86 Attenzione: prestare attenzione durante l’utilizzo dei tubi di polimero pressurizzati: • Indossare sempre occhiali da lavoro protettivi nei pressi di tubi di polimero pressurizzati. • Estinguere ogni fonte di ignizione circostante. • Non utilizzare tubi soggetti che hanno subito sollecitazioni eccessive o son stati incurvati.
  • Page 87 Warnings that apply to all Waters instruments...
  • Page 88 Warning: The user shall be made aware that if the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired. Attention: L’utilisateur doit être informé que si le matériel est utilisé d’une façon non spécifiée par le fabricant, la protection assurée par le matériel risque d’être défectueuses.
  • Page 89 Attenzione: per una buona protezione contro i rischi di incendio, sostituire i fusibili con altri dello stesso tipo e amperaggio. Advertencia: sustituya los fusibles por otros del mismo tipo y características para evitar el riesgo de incendio. Warnings that apply to all Waters instruments A-11...
  • Page 90 Warning: To avoid possible electrical shock, disconnect the power cord before servicing the instrument. Attention: Afin d’éviter toute possibilité de commotion électrique, débranchez le cordon d’alimentation de la prise avant d’effectuer la maintenance de l’instrument. Vorsicht: Zur Vermeidung von Stromschlägen sollte das Gerät vor der Wartung vom Netz getrennt werden.

  • Page 91: Electrical And Handling Symbols

    Electrical and handling symbols Electrical symbols These can appear in instrument user manuals and on the instrument’s front or rear panels. Electrical power on Electrical power off Standby Direct current Alternating current Protective conductor terminal Frame, or chassis, terminal Fuse Recycle symbol: Do not dispose in municipal waste.

  • Page 92: Handling Symbols

    Handling symbols These handling symbols and their associated text can appear on labels affixed to the outer packaging of Waters instrument and component shipments. Keep upright! Keep dry! Fragile! Use no hooks! A-14 Safety Advisories...

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