Waters ACQUITY UPLC Operator's, Overview And Maintenance Manual page 63

To perform the system, acid-cleansing flush
Caution:
path.
1. Remove the sample and solvent manager bottle filters.
2. Place all lines A1, A2, B1, B2 seal wash, weak needle wash and strong
needles in 50:50 methanol:water.
3. Prime the solvent lines for 5 minutes each.
4. Prime the seal wash.
5. Prime the wash syringes and sample syringe for 4 cycles.
6. Connect a pressure restrictor in the fluid path, after the injector, to
create 13,800 kPa (138 bar, 2000 psi) backpressure in the system.
7. Transfer 1 mL of mobile phase to an autosampler vial, and place the vial
in position 1:A,1.
8. Create an instrument method adopting the following parameter
settings:
• Flow rate = 0.5 mL/min
• Gradient composition 50% A1:50% B1
• Full loop injection
9. Make 30 full-loop injections from the vial containing the mobile phase.
10. Set the run time to 0.5 minutes.
Expect this step to take approximately 30 minutes.
Tip:
11. Repeat steps 1 through 8 using 100% isopropanol as the solvent.
Requirement:
step. Route the restrictor to waste.
12. Repeat steps 1 through 8 using 100% water as the solvent.
Requirement:
prior to performing the phosphoric acid wash.
13. Repeat steps 1 through 8 using 30:70 (v/v) phosphoric acid/water as the
solvent.
Failure to remove the bottle filters contaminates the flow
Do not pass effluent through optical detector for this wash
Remove the Seal wash line from the mobile phase bottle
Maintaining the detector 51