Waters 2475 Manuals

Manuals and User Guides for Waters 2475. We have 2 Waters 2475 manuals available for free PDF download: Operator's Manual, Overview And Maintenance Manual

Waters 2475 Operator's Manual (228 pages)

Multi Fluorescence Detector

Brand: Waters | Category: Security Sensors | Size: 2 MB

Copyright Notice
2
Trademarks
2
Customer Comments
3
Safety Considerations
3
Safety Advisories
4
Operating this Instrument
4
Audience and Purpose
4
Intended Use of the Instrument
5
Calibrating
5
ISM Classification
5
ISM Classification: ISM Group 1 Class B
5
EC Authorized Representative
6
Table of Contents
7
Theory of Operation
16
Fluorescence Theory
16
Fluorescence Detection
17
Overview
17
Excitation Sources
17
Types of Light Sources
17
Excitation Wavelength Selection
17
Exciting the Sample
18
Flow Cell
18
Measuring Fluorescence
18
Quantitation
18
Emission Wavelength Selection
18
Photomultiplier Tube
18
Scanning
19
Multichannel Operation
19
Fluorescence Data
19
References
20
Detector Description
21
Features
21
Principles of Operation
23
Detector Optics
23
Optics Assembly Light Path
24
Photomultiplier (PMT) Calibration
26
PMT Sensitivity
26
Filtering Noise
26
Electronics
27
Wavelength Verification and Test
28
Operational Modes
28
Single-Channel Mode
28
Multichannel Mode
29
Spectrum Scanning
30
Lamp Energy and Performance
30
Auto-Optimize Gain
31
Method Optimization
32
Example of Recommended Method Development Approach
33
Ensuring Gain Optimization for each Peak of Interest
34
Startup Diagnostic Tests
35
Mobile-Phase Solvent Degassing
35
Wavelength Selection
36
Setting up the Detector
37
Before You Begin
38
Installing the Detector
39
Plumbing the Detector
39
Connecting Columns
39
Assembling Fittings
40
Making Tubing Connections
41
Making Signal Connections
41
Component Connection Overview
42
Connecting the Ethernet Cable
43
Choosing Signal Connections
46
Making I/O Signal Connections
47
Signal Connections
48
Connecting an Alliance Separations Module
50
Connecting RS-232 Devices
54
Connecting Ethernet Devices
56
Connecting Other Devices
59
Required Materials
59
Connecting Cables
59
Connecting a Data System Using a Bus SAT/IN Module
60
Connecting a 746 Data Module
63
Connecting a Chart Recorder
64
Connecting a 600-Series Pump
65
Connecting a 717Plus Autosampler
69
Connecting to the Electricity Source
72
Using the Detector
73
Starting the Detector
74
Initializing the Detector
74
Startup Failure
75
Idle Mode
76
Using the Operator Interface
76
Using the Display
76
Fluorescence and Message Icons
77
Using the Keypad
81
Navigating the User Interface
88
Navigating to and from the Home Screen
88
Preparing to Start a Run
90
Setting up a Run
90
Accessing Primary and Secondary Functions
91
Operating the Trace and Scale Functions
95
Configuring the Detector
97
Configuring Event Inputs and Contact Closures
97
Setting Pulse Periods
98
Setting the Display Contrast
99
Displaying System Information
100
Using Online Help
100
Operating the Detector
100
Two Operating Modes
101
Standalone Operation
101
Remote Control Operation Via Ethernet Connection Using 2475 Instrument Control Software
106
Verifying the Detector
106
Manual Wavelength Calibration
107
Normalizing Emission Units
108
Operating the Detector in Single-Channel Mode
109
Operating the Detector in Multichannel Mode
110
Setting Gain and EUFS
112
Programming Methods and Events
115
Storing Methods
115
Programming Timed Events
116
Programming Threshold Events
119
Storing a Method
121
Retrieving a Method
122
Viewing Events Within a Method
122
Resetting a Method
123
Clearing Events
123
Scanning Spectra
124
Types of Scanning
124
Before You Begin
124
Scanning New Spectra
129
Parameters Used for Sample and Zero-Scans
130
Programming a Zero-Scan
131
Running a Sample Scan
132
Scanning Using a Static Flow Cell
136
Managing Results
137
Storing a Spectrum
137
Getting Information about a Stored Spectrum
138
Reviewing a Stored Spectrum
138
Creating a Difference Spectrum (Subtracting a Spectrum)
139
Replaying a Spectrum
139
Conserving Lamp Life
139
Manually Extinguishing the Lamp
140
Manually Lighting the Lamp
141
Using a Timed Event Method to Program the Lamp
142
Shutting down the Detector
142
Maintenance Procedures
144
Contacting Waters Technical Service
144
Maintenance Considerations
144
Safety and Handling
144
Spare Parts
145
Routine Maintenance
145
Removing the Front-Left-Hand Panel Cover
146
Inspecting, Cleaning, and Replacing the Flow Cell
147
Flushing and Passivating the Flow Cell
147
Removing the Flow Cell Assembly
148
Replacing the Flow Cell
149
Replacing the Lamp
150
When to Replace the Lamp
150
Removing the Lamp
151
Installing the New Lamp
154
Recording the New Lamp's Serial Number
155
Replacing the Fuses
156
Cleaning the Instruments Exterior
157
Error Messages, Diagnostic Tests, and Troubleshooting
159
Startup Error Messages
160
Operational Error Messages
161
User-Selected Diagnostic Tests and Settings
166
Overview of Diagnostic Tests and Settings
166
Sample and Reference Energy Diagnostic Tests
169
Raman Signal-To-Noise Test Diagnostic Test
170
Input and Output Diagnostic Tests and Settings
170
Change-Lamp Function
173
Testing the Keypad
174
Testing the Display
175
Other Diagnostic Tests and Settings
175
Generating Test Peaks
175
Overriding the Optical Filter Setting
176
Reducing PMT Sensitivity
177
Troubleshooting
178
Introduction
178
Information Needed When You Contact Waters
178
Power Surges
179
Hardware Troubleshooting
179
A Safety Advisories
184
Warning Symbols
184
Task-Specific Hazard Warnings
184
Specific Warnings
185
Caution Symbol
187
Warnings that Apply to All Waters Instruments
188
Electrical and Handling Symbols
194
Electrical Symbols
194
Handling Symbols
195
B Specifications
197
C Solvent Considerations
202
Introduction
202
Clean Solvents
202
Solvent Quality
202
Preparation Checklist
202
Water
202
Buffers
203
Tetrahydrofuran (THF
203
Solvent Miscibility
203
How to Use Miscibility Numbers
205
Buffered Solvents
206
Solvent Viscosity
206
Mobile Phase Solvent Degassing
207
Gas Solubility
207
Solvent Degassing Methods
208
Solvent Degassing Considerations
209
UV Cutoffs for Common Solvents
210
Index
215

Waters 2475 Overview And Maintenance Manual (149 pages)

Multi-Wavelength Fluorescence Detector

Brand: Waters | Category: Laboratory Equipment | Size: 1 MB

General Information
2
Copyright Notice
2
Trademarks
2
Customer Comments
2
Contacting Waters
3
Safety Considerations
3
Safety Hazard Symbol Notice
4
Considerations Specific to the Instrument
4
Electrical Power Safety Notice
4
Equipment Misuse Notice
4
Safety Advisories
4
Operating the Device
4
Applicable Symbols
5
Audience and Purpose
6
Calibrating
6
Quality Control
6
EMC Considerations
7
Canada Spectrum Management Emissions Notice
7
ISM Classification: ISM Group 1 Class B
7
Table of Contents
8
Overview
14
Fluorescence Theory
15
Fluorescence Detection
15
Overview of Fluorescence Detection
15
Excitation Sources
16
Types of Light Sources
16
Excitation Wavelength Selection
16
Exciting the Sample
16
Flow Cell
16
Measuring Fluorescence
17
Quantitation
17
Emission Wavelength Selection
17
Photomultiplier Tube
17
Scanning
17
Multichannel Operation
17
Fluorescence Data
17
Energy Units
18
References
18
Principles of Operation
19
Detector Optics
19
Optics Assembly Light Path
21
PMT Calibration
22
PMT Sensitivity
22
Filtering Noise
23
Electronics
23
Wavelength Verification and Test
24
Operational Modes
24
Single Channel Mode
24
Multichannel Mode
25
Spectrum Scanning Mode
26
Lamp Energy Performance
26
Auto-Optimize Gain
27
Method Optimization
28
Example of Recommended Method Development Approach
28
Ensuring Gain Optimization for each Peak of Interest
29
Startup Diagnostic Tests
30
Mobile Phase Solvent Degassing
30
Wavelength Selection
31
Setting up the Detector
32
Before You Begin
32
Installing the Detector
32
Connecting to the Electricity Source
34
Plumbing the Detector
35
Connecting Columns in HPLC Systems
35
Connecting Columns in Arc Premier Systems
36
Assembling Fittings (HPLC Systems Only)
36
Making Tubing Connections in HPLC Systems
37
Making Tubing Connections in Arc Premier Systems
37
Making Signal Connections
38
Component Connection Overview
38
Connecting the Ethernet Cable
39
Choosing Signal Connections
41
Making I/O Signal Connections
41
Signal Connections
42
Connecting an Alliance E2695 Separations Module
45
Starting a Method
47
Making RS-232 Signal Connections
48
Connecting Other Devices
50
Connecting Cables
50
Connecting a Data System Using an E-SAT/IN Module
50
Connecting a Waters Fraction Collector
52
Using the Detector
55
Starting the Detector
55
Initializing the Detector
55
Startup Failure
56
Idle Mode
56
Using the Operator Interface
57
Using the Display
57
Fluorescence and Message Icons
58
Using the Keypad
60
Navigating the User Interface
65
Navigating to and from the Home Screen
65
Preparing to Start a Run
67
Setting up a Run
67
Accessing Primary and Secondary Functions
67
Operating the Trace and Scale Functions
71
Configuring the Detector
73
Configuring Event Inputs and Contact Closures
73
Setting Pulse Periods
74
Setting the Display Contrast
75
Displaying System Information
75
Using Online Help
76
Operating the Detector
76
Two Operating Modes
76
Stand-Alone Operation
76
Remote Control Operation for 474 Emulation Mode Via RS-232
77
Operation Details
80
Remote Control Operation Via Ethernet Connection Using Instrument Control Software
80
Verifying the Detector
80
Manual Wavelength Calibration
81
Normalizing Emission Units
82
Operating the Detector in Single-Channel Mode
83
Operating the Detector in Multichannel Mode
83
Setting Gain and EUFS
84
Programming Methods and Events
88
Storing Methods
88
Programming Timed Events
89
Programming Threshold Events
91
Storing a Method
92
Retrieving a Method
93
Viewing Events Within a Method
93
Resetting a Method
94
Clearing Events
94
Scanning Spectra
95
Types of Scanning
95
Before You Begin
95
Scanning New Spectra
99
Parameters Used for Sample and Zero-Scans
100
Programming a Zero-Scan
101
Running a Sample Scan
102
Scanning Using a Static Flow Cell
105
Managing Results
106
Storing a Spectrum
106
Displaying Information about a Stored Spectrum
107
Reviewing a Stored Spectrum
107
Creating a Difference Spectrum (Subtracting a Spectrum)
107
Replaying a Spectrum
108
Conserving Lamp Life
108
Manually Extinguishing the Lamp
109
Manually Lighting the Lamp
110
Using a Timed Event Method to Program the Lamp
111
Shutting down the Detector
111
Maintenance
112
Maintenance Considerations
112
Safety Advisories
112
Spare Parts
112
Routine Maintenance
112
Inspecting, Cleaning, and Replacing the Flow Cell
113
Flushing and Passivating the Flow Cell
114
Removing the Flow Cell Assembly
114
Replacing the Flow Cell
115
Replacing the Lamp
116
When to Replace the Lamp
116
Removing the Lamp
117
Installing the New Lamp
118
Recording the New Lamp's Serial Number
119
Replacing the Fuses
120
To Replace the Fuses
121
Cleaning the Detector's Exterior
121
Error Messages, Diagnostic Tests, and Troubleshooting
122
Startup Error Messages
122
Operational Error Messages
122
User-Selected Diagnostic Tests and Settings
123
Overview of Diagnostic Tests and Settings
123
Sample and Reference Energy Diagnostic Tests
125
Raman Signal-To-Noise Diagnostic Test
126
Input and Output Diagnostic Tests and Settings
126
Change-Lamp Function
129
Testing the Keypad
129
Testing the Display
130
Other Diagnostic Tests and Settings
130
Generating Test Peaks
131
Overriding the Optical Filter Setting
131
Reducing PMT Sensitivity
132
Troubleshooting
133
Introduction
133
Information Needed When You Contact Waters
133
Power Surges
134
Hardware Troubleshooting
134
A Safety Advisories
136
Warning Symbols
136
Specific Warnings
137
Biohazard Warning
138
Notices
138
Bottles Prohibited Symbol
139
Required Protection
139
Warnings that Apply to All Waters Instruments and Devices
139
Warnings that Address the Replacement of Fuses
143
Electrical Symbols
145
Handling Symbols
146
B Specifications
147
Physical Specifications
147
Environmental Specifications
147
Electrical Specifications
148
Performance Specifications
149

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