Waters ACQUITY UPLC Overview And Maintenance Manual
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Waters ACQUITY UPLC Overview And Maintenance Manual

Sample manager flow through needle
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ACQUITY UPLC Sample Manager -
Flow Through Needle
Overview and Maintenance Guide
715005051
Copyright © Waters Corporation 2019
Revision D
All rights reserved

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Summary of Contents for Waters ACQUITY UPLC

  • Page 1 ACQUITY UPLC Sample Manager - Flow Through Needle Overview and Maintenance Guide 715005051 Copyright © Waters Corporation 2019 Revision D All rights reserved...

  • Page 2: General Information

    This document is believed to be complete and accurate at the time of publication. In no event shall Waters Corporation be liable for incidental or consequential damages in connection with, or arising from, its use. For the most recent revision of this document, consult the Waters website (www.waters.com).

  • Page 3: Contacting Waters

    Contacting Waters Contact Waters with enhancement requests or technical questions regarding the use, transportation, removal, or disposal of any Waters product. You can reach us via the Internet, telephone, fax, or conventional mail. Waters contact information Contacting medium Information...

  • Page 4: Considerations Specific To The Sm-Ftn

    To avoid electric shock, observe these precautions: • Use SVT-type power cords in the United States and HAR-type power cords, or better, in Europe. For requirements elsewhere, contact your local Waters distributor. • Inspect the power cords for damage and replace them if necessary.

  • Page 5: Operating The Device

    Electrical and electronic equipment with this symbol may contain hazardous substances and should not be disposed of as general waste For compliance with the Waste Electrical and Electronic Equipment Directive (WEEE) 2012/19/EU, contact Waters Corporation for the correct disposal and recycling instructions For indoor use only No pushing June 11, 2019, 715005051 Rev.

  • Page 6: Audience And Purpose

    (SM-FTN). It gives an overview of the technology and operation of the SM-FTN. Intended use of the SM-FTN Waters designed the SM-FTN for use in liquid chromatography applications. The SM-FTN is not intended for use in diagnostic applications.

  • Page 7: Canada Spectrum Management Emissions Notice

    Class B products are suitable for use in both commercial and residential locations and can be directly connected to a low voltage, power-supply network. EC authorized representative Address Waters Corporation Stamford Avenue Altrincham Road Wilmslow SK9 4AX UK Telephone...

  • Page 8: Table Of Contents

    Table of contents General information .......................ii Copyright notice ............................. ii Trademarks............................ii Customer comments..........................ii Contacting Waters ..........................iii Safety considerations..........................iii Safety hazard symbol notice ......................iii Considerations specific to the SM-FTN ..................iv FCC radiation emissions notice...................... iv Electrical power safety notice ......................iv Equipment misuse notice .......................
  • Page 9 2 Preparation and operation ..................21 2.1 Installation recommendations for fittings..................21 2.1.1 Assembling new fittings ....................... 22 2.1.2 1/4-28 flangeless fitting with ferrule ..................23 2.1.3 1/4-28 flangeless fitting with 2-piece ferrule ................ 24 2.1.4 One-piece PEEK fitting ......................24 2.1.5 Stainless steel (gold-plated) fitting with long flats and 2-piece stainless steel ferrule (V- detail).............................24 2.2 Ventilation requirements .......................
  • Page 10 2.36 Selecting auto dilution ......................... 58 2.37 Responding to a leak-sensor alarm ................... 58 2.38 Diagnostic tests........................... 61 3 Maintenance ......................63 3.1 Contacting Waters Technical Service ..................63 3.2 Viewing module information ......................64 3.3 Recommended maintenance schedule..................64 3.3.1 Recommended maintenance schedule for the sample manager ........64 3.4 Spare parts............................
  • Page 11 A.1.1 Specific warnings ......................109 A.2 Notices............................110 A.3 Bottles Prohibited symbol ......................111 A.4 Required protection ........................111 A.5 Warnings that apply to all Waters instruments and devices ............111 A.6 Warnings that address the replacement of fuses ............... 115 A.7 Electrical symbols ........................117 A.8 Handling symbols ........................118 B Specifications......................119...

  • Page 12: Overview

    Overview You can submit samples for analysis on the ACQUITY UPLC system by loading microtiter plates or vials onto the rotary sample tray of the Sample Manager-Flow Through Needle (SM-FTN). Using a flow-through-needle mechanism, in which the needle is part of the high-pressure sample flow path, the sample manager injects the samples it draws from the plates and vials onto a chromatographic column.
  • Page 13 On/off switch – Powers the module on and off. Power LED – Indicates the power-on or power-off status of the module. This LED is green when power is on and unlit when power is off. Run LED – Indicates the run status. A steady green run LED indicates that injections are being run.
  • Page 14 Location of column heater leak sensor - The column heater leak sensor continuously monitors the column heater for leaks and stops the system flow when its optical sensor detects about 1.5 mL of accumulated, leaked liquid in its surrounding reservoir. Injection valve - A two-position, six-port injection valve.
  • Page 15 Sample compartment LED lighting - The compartment light automatically switches on when the SM-FTN’s sample compartment door is opened. It automatically switches off when the door is closed. The light can also be switched off via the console, to accommodate light- sensitive samples.

  • Page 16: Flow Path Through The System

    Ethernet switch Power input 1.2 Flow path through the system The following diagram shows how the SM-FTN functions as part of the ACQUITY UPLC system. Figure 1–5: Flow path through the system 1.3 Rθ positioning mechanism The Rθ (R-theta) positioning mechanism’s two axes control the orientation of the sample plates within the sample compartment and the relative position of the sample needle carriage.
  • Page 17 Figure 1–6: R-theta positioning mechanism, located below sample compartment Theta-rotary axis Belt Figure 1–7: R-linear axis, located inside sample compartment R-linear axis Z and Zp axis (needle and foot/pierce needle) Sample-needle carriage June 11, 2019, 715005051 Rev. D Page 17...

  • Page 18: Injection System

    1.4 Injection system The injection flow path includes the assemblies required to aspirate a sample and deliver it to the column. The process involves the needle, optional extension loop, sample syringe and syringe valve, injection valve, and injection/wash port. 1.5 Injection mechanics During an injection, this five-step sequence takes place: The needle moves to the sample vial and aspirates sample from it.

  • Page 19: Wash System

    Seal Locking nut Location of force sensor Spring cup Seal Needle Aluminum housing The following figure shows standard-injection-mode cycle time. Figure 1–9: Standard-injection-mode cycle time definition 1.5.1 Wash system The wash system cleans the exterior of the sample needle while it is inside the injection/wash port.

  • Page 20: Thermal System

    If you are concerned about material on the outside of the sample needle damaging the seal or affecting the contact between the seal and the needle, perform the pre-injection wash, to further reduce carryover. 1.5.1.2 Post-injection, exterior needle wash Performed by default, this wash washes the exterior of the sample needle after an injection begins and the needle is in the seal position.

  • Page 21: Preparation And Operation

    Before you prepare the sample manager for operation, prepare the solvent manager. Tip: For instructions explaining how to prepare the solvent manager, see the ACQUITY UPLC Quaternary Solvent Manager Overview and Maintenance Guide or ACQUITY UPLC Binary Solvent Manager Operator's Overview and Maintenance Information.

  • Page 22: Assembling New Fittings

    • To prevent band spreading, ensure that the tubing is fully bottomed in the connection port before tightening the compression screw. • For easier accessibility, use compression fittings with long flats to attach tubes to the injector valve. • Perform the sample-syringe leak test whenever you replace or loosen fittings during maintenance (see the console online Help).

  • Page 23: 1/4-28 Flangeless Fitting With Ferrule

    Insert the tube into the connection port. Rotate the compression screw, clockwise, into the connection port until the screw is finger- tight. Using a permanent marker, mark the compression screw at the 12-o’clock position. Mark the connection port at the 9-o’clock position. Ensure that the tubing makes contact with the bottom of the connection port, and use the 1/4-inch open-end wrench to rotate the compression screw clockwise 3/4-turn until the two marks line up.

  • Page 24: 1/4-28 Flangeless Fitting With 2-Piece Ferrule

    2.1.3 1/4-28 flangeless fitting with 2-piece ferrule First use or re-installed Compression screw 2-piece ferrule Tighten the fitting finger-tight. 2.1.4 One-piece PEEK fitting Figure 2–1: First use or reinstalled Compression screw Tighten the fitting finger-tight. 2.1.5 Stainless steel (gold-plated) fitting with long flats and 2-piece stainless steel ferrule (V-detail) First use June 11, 2019, 715005051 Rev.
  • Page 25 Long flats Compression screw 2-piece stainless steel ferrule Tighten the fitting finger-tight plus an additional 3/4-turn using a 1/4-inch open-end wrench. For detailed instructions about assembling new fittings, see Assembling new fittings. Tip: To prevent band spreading, ensure that the tubing is fully bottomed in the connection port before you tighten the compression screw.

  • Page 26: Ventilation Requirements

    Reinstalled tightening 2.2 Ventilation requirements Allow at least 15.2 cm (6.0 inches) clearance at the rear, and at least 6.4 cm (2.5 inches) clearance on the right-hand side of the sample manager for ventilation. 2.3 Stacking system modules without interlocking features This procedure applies to system modules that are not equipped with interlocking features.

  • Page 27: Stacking System Modules With Interlocking Features

    Feet on underside of module being stacked Indents on top side of previously added module Carefully lower the module so that the feet rest in the indents. Important: To maintain the integrity of the system stack and integrated waste system, ensure that the feet of the upper module rest in the indents of the lower module.

  • Page 28: Drainage System Requirements

    Alignment pins Alignment slots Lower the front of the module that you are adding so that its front alignment pin rests in the front alignment slot on the previously added module. Repeat steps 1 and 2 for the remaining system modules. 2.5 Drainage system requirements When plumbing the sample manager, observe these drainage system requirements: •...
  • Page 29 See also: The ACQUITY UPLC Binary Solvent Manager PLUS Overview and Maintenance Guide, the µBinary Solvent Manager Overview and Maintenance Guide, or the ACQUITY UPLC Quaternary Solvent Manager PLUS Series Overview and June 11, 2019, 715005051 Rev. D Page 29...

  • Page 30: Electricity Source

    (CH-30A), receive their power from the sample manager via the interconnect cable. Recommendation: Use a line conditioner and uninterruptible power supply (UPS) for optimum, long-term, input-voltage stability. Contact Waters to ensure the correct selection and size. To connect to a wall electricity source: Connect the female end of the power cord to the receptacle on the rear panel of the module.

  • Page 31: Connecting Signal Cables

    To avoid electric shock, observe these precautions: • Use SVT-type power cords in the United States and HAR-type power cords, or better, in Europe. For requirements elsewhere, contact your local Waters distributor. • Inspect the power cords for damage and replace them if necessary.
  • Page 32 Figure 2–6: Inserting connector into connector port Connector port Connector Using the flat-blade screwdriver, attach the positive and negative leads of the signal cable to the connector. Tip: Refer to the cable-connection label affixed to the rear panel of the module. Figure 2–7: Positive and negative lead connections Screw Connector...

  • Page 33: Input/Output Signal Connectors

    Figure 2–8: Grounding cable fork terminal on grounding stud Fork terminal Locking nut Grounding stud 2.8.1 Input/output signal connectors Refer to the cable-connection label affixed to the rear panel of the module. Note: A contact closure output connection (Inject Start) from the sample manager is required to trigger a mass spectrometer, an ACQUITY 2996 PDA detector, or an ACQUITY ELS detector running under MassLynx software control to start.

  • Page 34: Installing The Optional Lower Leak Sensor

    2.9.1 Installing the optional lower leak sensor Warning: Observe Good Laboratory Practice (GLP) at all times, particularly when working with hazardous materials. Consult the Material Safety Data Sheets regarding the solvents you use. Additionally, consult the safety representative for your organization regarding its protocols for handling such materials.
  • Page 35 Figure 2–9: Aligning leak sensor T-bar with slot Leak sensor T-bar Slot Slide the leak sensor ribbon cable into the slot on the left of the leak sensor reservoir. Figure 2–10: Leak sensor ribbon cable in slot June 11, 2019, 715005051 Rev. D Page 35...
  • Page 36 Leak sensor ribbon cable Leak sensor Slot Leak sensor ribbon cable Figure 2–11: Leak sensor ribbon cable routing Slot Leak sensor ribbon cable Leak sensor Attach the leak sensor connector to the lower port on the front of the device. Figure 2–12: Attaching leak sensor connector Leak sensor port on front of device Leak sensor connector...

  • Page 37: Installing The Optional Upper Leak Sensor

    In the console, select Sample Manager > Control > Reset module to reset the sample manager. In the console, enable the leak sensor (or sensors) to activate leak-detection capability (see the console online Help). 2.9.2 Installing the optional upper leak sensor Warning: Observe Good Laboratory Practice (GLP) at all times, particularly when working with hazardous materials.

  • Page 38: Calibrating The Needle

    Figure 2–13: Aligning leak sensor T-bar with slot Leak sensor T-bar Slot Attach the leak sensor connector to the upper port on the front of the device. Figure 2–14: Attaching leak sensor connector Leak sensor connector Leak sensor port on front of device Power-on the sample manager.

  • Page 39: Purge Solvent

    Required tools and materials • Business card To calibrate the needle, do the following: Open the sample manager door. Remove the plates from the trays. Click Maintain > Calibrate needle. Click Start, and then, in the confirmation window, click OK. Slide a business card under the needle.

  • Page 40: General Purge And Wash Solvent Guidelines

    See also: Wash system 2.13 General purge and wash solvent guidelines For best performance, follow these guidelines when selecting purge and wash solvents. Otherwise, you can increase the risk of carryover. The guidelines do not prohibit all other solvent combinations, however, which you can run with lower performance expectations or by manipulating injection parameters.

  • Page 41: Priming The Sample Manager

    To prime the sample syringe and wash solvent: From the system view of the ACQUITY UPLC Console, select the sample manager, and then click Control > Prime . Alternative: In the data application, in the control panel of the sample manager, right- click and then click Prime.

  • Page 42: Washing The Needle's Exterior

    Table 2–3: Priming parameter values (continued) Parameter Wash solvent Purge solvent Recommended priming: changing solvents 180 seconds 100-µL syringe: 50 cycles 250-µL syringe: 20 cycles 500-µL syringe: 10 cycles Note: Each priming cycle requires approximately 30 seconds. When the reported system status is “Idle", priming is finished. 2.15 Washing the needle's exterior The wash system cleans the outside of the sample needle while it is inside the injection/wash port.

  • Page 43: Loading Sample Plates

    Use plates that meet ANSI/SBS standards. Tip: Vial positions V1 through V4, located on the right-hand and left-hand sides of the sample tray, accommodate 4-mL vials. Contact Waters for inserts that allow you to use 2-mL vials in these positions. Required tools and materials •...
  • Page 44 Figure 2–15: Pulling sample tray out of sample compartment Plate selector switch Position for a 4-mL vial (V1 through V4) Sample tray pulled out Load the plate onto the tray so that well position A,1 is at the rear, left-hand corner, and the forward edge of the plate is behind the sample tray handle.
  • Page 45 Figure 2–16: Loading sample plate onto sample tray Well position A,1 Forward edge of sample plate Sample tray handle Figure 2–17: Sample plate vial positions Slide the tray in until it clicks into place. June 11, 2019, 715005051 Rev. D Page 45...

  • Page 46: Observing Vial And Plate Recommendations

    Close the sample-compartment door. 2.17 Observing vial and plate recommendations Waters recommends that you observe these usage guidelines for sample vials and plates in the sample manager: • Vials • Use only Waters-certified vials.

  • Page 47: Sample Chamber Considerations

    2.19 Choosing sample needles Waters offers multiple needle sizes, so you can choose the best option for your injection volume and sample viscosity. The 15-µL needle provides the best injection volume accuracy, while the 30-µL needle allows for sample aspiration rates up to four times faster.

  • Page 48: Installing And Replacing Extension Loops

    • 250 µL • 1000 µL Recommendation: The maximum injection volume is 75% of the total loop and needle volume. Injections larger than the recommended maximum volume can result in reduced sample recovery. Apply the following formula to calculate the maximum injection volume: Maximum injection volume (µL) = [loop volume (µL) + needle volume (µL)] ×...
  • Page 49 Figure 2–19: Injection valve Needle tubing (or extension loop tubing, if installed) Fully insert the extension-loop tubing into port 4 of the injection valve, and then thread the fitting into the port. Holding the extension loop tubing against the bottom of the port, finger-tighten the extension-loop fitting.

  • Page 50: Modifying Needle And Extension Loop Configuration Parameters

    The sample manager performs multiple draws of the syringe plunger to aspirate samples larger than the syringe volume. This repetitive action increases the time required for sample aspiration. Waters therefore recommends choosing a syringe size that can accommodate the total sample volume in the syringe (and optional extension loop).

  • Page 51: Choosing A Draw Rate For The Sample Syringe

    2.25 Choosing a draw rate for the sample syringe The optimal draw rate for the syringe plunger depends on the volume and viscosity of the sample and the specified cycle time. Default draw rates depend on the needle size: • 15 µL needle: 30 µL/min •...

  • Page 52: Recovering Maximum Sample From Vials

    FTN > General tab > Advanced, and change the “Needle Placement (from bottom)” value. Vial type Minimum Description Part number needle placement (mm) Waters Supplied Screw Cap 12 × 32 mm Clear Total Rec 186000385C Total Recovery Vial pre-slit PTFE/Silicone Septa Waters Supplied Screw Cap 12 × 32 mm Clear Max Rec 186000327C Max.

  • Page 53: Revising A Plate Type

    186002639 slit PTFE/Silicone Septa See also: Waters Sample Vials and Accessories brochure on www.waters.com. 2.28 Revising a plate type The ANSI-48Tube0.65mLHolder plate type is defined in Empower software so that the needle does not use sample near the bottom of the vial, greatly increasing the residual volume. The plate type contains 0.65-mL tubes.

  • Page 54: Load-Ahead Option

    • To prevent loss of sample from the end of the needle, add a post-aspirate air gap of 0.1 to 0.2 µL. Waters does not recommend adding air gaps before or after the sample. An air gap of 3 µL or greater causes an extra peak in the chromatography.

  • Page 55: Loop-Offline Option

    Figure 2–22: Standard injection mode cycle time definition The following figure shows load-ahead mode cycle time after the first injection. Figure 2–23: Load-ahead mode cycle time definition Tip: The time buffer delay is a “wait time” that compensates for variations in the time required to load a sample.

  • Page 56: Determining When To Take The Needle And Extension Loop Offline

    Take the loop offline before the first gradient change reaches the injection valve or after the gradient returns to the initial conditions. 2.32 Determining when to take the needle and extension loop offline When the needle and extension loop are eliminated from the flow path, they contain the mobile phase composition.

  • Page 57: Choosing The Load-Ahead And Loop-Offline Options

    result, the gradient can fail to remove all sample from the needle, resulting in low sample recovery and an increased risk of carryover. 2.33 Choosing the load-ahead and loop-offline options To choose the load-ahead and loop-offline options: In the instrument method editor, click the ACQ-FTN tab, and then click the General tab. Select the check boxes for the load-ahead and loop-offline options.

  • Page 58: Auto Additions

    See also: For more information about controlling contamination in chromatographic systems, see the Controlling Contamination in LC/MS Systems Best Practices (715001307) on the Waters website. 2.35 Auto additions If you use Empower 3 to control the sample manager, you can use the Auto additions function to make an injection composed of sample from as many as 10 vials, one of which is the sample vial.
  • Page 59 If the message reads “Leak Detected”, locate the source of the leak and make the repairs necessary to stop it. For additional information, see the ACQUITY UPLC Column Heater- Active Overview and Maintenance Guide, ACQUITY UPLC 30-cm Column Heater-Active...
  • Page 60 Figure 2–24: Leak-sensor assembly Serrations Ribbon cable Prism Tip: If you cannot easily manipulate the leak sensor after removing it from its reservoir, detach the connector from the front of the device (see Replacing the leak sensor). Use a nonabrasive, lint-free wipe to dry the leak-sensor prism. Roll up a nonabrasive, lint-free wipe and use it to absorb the liquid from the leak-sensor reservoir and its surrounding area.

  • Page 61: Diagnostic Tests

    Figure 2–26: Aligning lower leak sensor T-bar with slot T-bar Slot in leak-sensor reservoir 10. If you detached the connector from the front of the device, reattach it. Figure 2–27: Attaching leak sensor connector Leak sensor connector Leak sensor port on front of device 11.
  • Page 62 • Parking the sample needle and injection valve, which you do before storing the system or replacing a needle or valve. See also: • Quaternary Solvent Manager Overview and Maintenance Guide or ACQUITY UPLC Binary Solvent Manager Operator's Overview and Maintenance Information for information on the solvent manager’s leak test.

  • Page 63: Maintenance

    Help. 3.1 Contacting Waters Technical Service If you are located in the USA or Canada, report malfunctions or other problems to Waters Technical Service (800-252-4752). From elsewhere, phone the Waters corporate headquarters in Milford, Massachusetts (USA) or contact your local Waters subsidiary. The Waters website includes phone numbers and email addresses for Waters locations worldwide.

  • Page 64: Viewing Module Information

    Be prepared to provide the serial numbers of the modules in your system when you contact Waters customer support. To view module information: In the console, select a module from the system tree.

  • Page 65: Spare Parts

    As necessary cloth, or paper dampened with water 3.4 Spare parts To ensure that your system operates as designed, use only Waters Quality Parts. Visit www.waters.com/wqp for information about Waters Quality Parts, including how to order them. 3.5 Safety and handling...

  • Page 66: Replacing The Leak Sensor

    3.7 Replacing the leak sensor The sample manager is fitted with two leak sensors, bottom and top, referred to as the lower and upper leak sensors. The procedures for replacing the leak sensors are identical. Warning: Observe Good Laboratory Practice (GLP) at all times, particularly when working with hazardous materials.
  • Page 67 Grasp the leak sensor by its serrations and pull upward on it to remove it from its reservoir. Figure 3–2: Leak sensor serrations Serrations Unpack the new leak sensor. Align the leak sensor T-bar with the slot in the side of the leak sensor reservoir and slide the leak sensor into place.

  • Page 68: Replacing The Seal

    Leak sensor connector Leak sensor port on front of device Power-on the sample manager. In the Console, select the sample manager, and then click Control > Reset SM to reset the device. 10. In the Console, enable the leak sensor to activate its leak-detection capability. 3.8 Replacing the seal Warning: To avoid personal contamination with biologically hazardous or toxic compounds,...
  • Page 69 Using the T20 TORX driver, loosen the two captive screws that secure the access panel, and then remove the panel. Figure 3–5: Captive screws on access panel Captive screws (2) Using the 1/4-inch open-end wrench, unscrew the seal-port tube’s fitting from port 1 of the injection valve.
  • Page 70 Figure 3–7: Inject/wash station assembly Inject/wash station assembly Screw Note: To ensure proper reinstallation of the inject/wash station assembly, note the location and routing of the wash tubing, seal-port tube, and load-cell cable before removing Figure 3–8: Tubing and cable locations Wash tubing Seal-port tube Load-cell cable...
  • Page 71 Remove the wash tubing from the clip on the inside wall of the sample compartment. Figure 3–9: Wash tubing secured by clip on sample-compartment wall Wash tubing Clip Notice: To avoid damaging the adapter fitting, ensure that you hold it while unscrewing the wash-tube fitting.
  • Page 72 Figure 3–11: Sliding support sleeve out of the housing Support sleeve Seal-port tube Slot Spring cup Load-cell cable Spring Housing Locking nut 11. Place the seal port’s housing in its original location on the sample-compartment floor. Result: Doing so helps ensure that the spring remains in the proper position. Note: The spring can fall out of the spring cup if the seal port's housing is not placed in its original location on the sample-compartment floor.
  • Page 73 Figure 3–12: Wrench placement locations Place the 1/2-inch open-end wrench here on the PEEK support sleeve. Place the 5/16-inch open-end wrench here on the stainless steel locking nut. 14. Loosen the locking nut and unscrew it from the support sleeve. Figure 3–13: Removing locking nut from support sleeve Support sleeve Tube/port assembly...
  • Page 74 Figure 3–14: Removing the seal from seal port Seal Locking nut Seal port Notice: To prevent contaminating system components, wear clean, chemical- resistant, powder-free gloves, and work on a clean surface when replacing the seal. 16. Insert the new seal into the seal port. The seal is keyed, ensuring its correct installation, as shown in the following image.
  • Page 75 Figure 3–16: Seal notch and pin Seal Notch Seal cup 17. Finger-tighten the locking nut into the support sleeve. Figure 3–17: Locking nut on support sleeve Support sleeve Locking nut 18. Place the 1/2-inch open-end wrench on the support sleeve to hold the sleeve in place. Notice: To avoid damaging the seal port tube, do not excessively twist the tube.
  • Page 76 Figure 3–18: Wrench placement locations Place the 1/2-inch open-end wrench here on the support sleeve. Place the 5/16-inch open-end wrench here on the locking nut. 20. Ensure that the seal-port tube is bent at 90 degrees and that it remains in line with the threaded hole in the support sleeve.
  • Page 77 Seal-port tube Step in locking nut 21. Slide the seal-port tube into the slot on the side of the housing. Figure 3–20: Sliding seal-port tube into slot Support sleeve Seal-port tube Slot Spring cup Load-cell cable Spring Housing Locking nut Requirement: Ensure that the three prongs on the locking nut are seated inside the spring.
  • Page 78 Figure 3–21: Prongs seated inside spring Prong (3) Locking nut Spring 22. Slide the support sleeve into the housing, ensuring that the fitting hole on the support sleeve aligns with the slot on the housing. Figure 3–22: Support sleeve in housing Fitting hole Slot Notice:...
  • Page 79 Figure 3–23: Adapter fitting in support sleeve Adapter fitting Support sleeve 24. Ensure that the seal-port tube is bent upward, at a 90-degree angle, and that it is approximately 2.5 cm (1.0 inch) from the housing. Note: To avoid binding the seal-port tube, ensure that the tube does not contact either side of the inject/wash station assembly.
  • Page 80 Figure 3–25: Wash-tube fitting in adapter fitting Adapter fitting Wash-tube fitting 26. Place the inject/wash station assembly in its original location on the sample-compartment floor and align the screw hole with the hole in the floor, ensuring that the wash drain of the injection port is in the needle-wash basin.
  • Page 81 Figure 3–27: Inject/wash station assembly Inject/wash station assembly Screw 30. Ensure that all cables are routed so that they do not interfere with the operation of the needle-carriage home sensor for the needle carriage. Figure 3–28: Needle-carriage home sensor Needle-carriage home sensor 31.
  • Page 82 Figure 3–29: Tubing and cable locations Wash tubing Seal-port tube Load-cell cable 32. Screw the seal port’s fitting into port 1 of the injection valve, and then use the 1/4-inch open-end wrench to tighten the fitting 1/4-turn beyond finger-tight. Figure 3–30: Seal-port tube location on injection valve June 11, 2019, 715005051 Rev.
  • Page 83 Seal-port tube 33. Reinstall the access panel and use the T20 TORX driver to tighten the two screws that secure the panel to the front of the unit. Requirement: Ensure that the seal-port tube and sample-needle tubing are routed through the gap in the access panel and that they do not cross each other. Figure 3–31: Tubing routed through access-panel gap Seal-port tube Access-panel gap...

  • Page 84: Replacing The Sample Needle And Needle Guide

    3.9 Replacing the sample needle and needle guide Warning: To avoid personal contamination with biologically hazardous or toxic compounds, wear clean, chemical-resistant, powder-free gloves when performing this procedure. Warning: To avoid eye injury, use eye protection when performing this procedure. Required tools and materials •...
  • Page 85 Figure 3–32: Access panel captive screws Captive screws (2) Using the 1/4-inch open-end wrench, unscrew the fitting of the needle tubing or extension loop from port 4 of the injection valve. Figure 3–33: Injection valve Needle tubing (or extension loop tubing, if installed) Inside the sample compartment, open the cover of the needle-tubing guide channel, located on the roof of the compartment, by inserting your finger in the finger slot and rotating the cover, as shown below.
  • Page 86 Figure 3–34: Needle-tubing guide cover in closed position Finger slot Location of needle tubing Needle-tubing guide channel Needle-tubing guide cover Figure 3–35: Needle-tubing guide cover in open position Needle-tubing guide cover Location of needle tubing Needle-tubing guide channel Using a T10 TORX driver, loosen the screw on the needle-tubing clamp and remove the needle tubing from the clamp.
  • Page 87 Figure 3–36: Needle tubing in needle-tubing clamp Screw Needle-tubing clamp Needle tubing 10. While holding the needle tubing, push the needle latch back to release the needle- mounting cylinder from its mounting cavity and the needle tubing from its notches. Figure 3–37: Needle latch in closed position Needle tubing Needle latch...
  • Page 88 Figure 3–38: Needle latch in open position Notches Needle tubing Latch Needle-mounting cylinder Warning: To avoid puncture injuries, handle sample needles, syringes, fused silica lines, and borosilicate tips with extreme care. Notice: To avoid damage to the end of the needle, do not touch or press the end of the sample needle.
  • Page 89 Figure 3–39: Needle tip in needle guide Needle guide Needle tip 12. Using the T6 TORX driver, loosen the needle-guide set screw, and then use the needle- nose pliers to remove the needle guide. Figure 3–40: Needle-guide set screw Needle guide Set screw 13.
  • Page 90 Figure 3–41: Conical side of needle guide Conical side of needle guide Set screw 14. Remove the protective sleeve from the needle tip. Figure 3–42: Sample-needle assembly Fitting ID sleeve Mounting sleeve Needle-mounting cylinder Needle tip Protective sleeve June 11, 2019, 715005051 Rev. D Page 90...
  • Page 91 Warning: To avoid puncture injuries, handle sample needles, syringes, fused silica lines, and borosilicate tips with extreme care. Notice: To avoid damage to the end of the needle, do not touch or press the end of the sample needle. 15. While holding the needle assembly in both hands to control its position within the sample compartment, insert the needle tip into the needle guide at the bottom of the needle mechanism, forming a loop with the tubing that extends back into the sample chamber, as shown below.
  • Page 92 Figure 3–44: Needle-mounting cylinder in mounting cavity Needle-mounting cylinder Mounting cavity 17. Route the needle tubing through the two notches below the Z-flag. Figure 3–45: Notches below Z-flag Notches Needle tubing Z-flag 18. Close the needle latch, as shown below, to secure the needle assembly. June 11, 2019, 715005051 Rev.
  • Page 93 Figure 3–46: Needle latch in closed position Needle tubing Needle latch 19. Ensure that the needle tubing is routed to the left-hand side of the needle carriage’s rails and that it is secured in the guide channel on the roof of the compartment. Figure 3–47: Location of needle tubing in guide channel Needle-tubing guide cover Location of needle tubing...
  • Page 94 Figure 3–48: Needle-tubing guide cover closed Finger slot Location of needle tubing Needle-tubing guide channel Needle-tubing guide cover 21. Secure the mounting sleeve in the opening of the tubing clamp with the fitting oriented toward the valve. 22. Using a T10 TORX driver, tighten the screw on the needle-tubing clamp, leaving the screw loose enough to be able to move the needle in the guide.
  • Page 95 Figure 3–49: Sample-needle assembly installed in sample compartment Mounting sleeve in needle-tubing clamp Needle-mounting cylinder in mounting cavity Needle tubing in needle guide Needle tip Needle tubing Lower RZZ mechanical rod Needle tubing in notches 23. Using the T10 TORX driver, tighten the screw on the needle-tubing clamp. Note: You might need to slightly rotate or twist the needle to ensure that it routes straight down the channel before tightening the screw on the needle-tubing clamp.
  • Page 96 Figure 3–50: Bending needle tubing at needle-tubing clamp Needle-tubing clamp Needle tubing Rounded tool 25. Reinstall the access panel and use the T20 TORX driver to tighten the two screws that secure the panel to the front of the unit. Requirement: Ensure that the seal-port tube and the sample-needle tubing are routed through the gap in the access panel.
  • Page 97 26. Using a rounded tool (such as the shaft of the T20 TORX driver), make a bend and connect the needle tubing to the extension-loop port or, if the extension loop is not installed, to port 4 on the injection valve. Figure 3–52: Needle tubing installed on injection valve Needle tubing 27.

  • Page 98: Replacing The Sample Syringe

    3.10 Replacing the sample syringe Warning: To avoid personal contamination with biologically hazardous or toxic compounds, wear clean, chemical-resistant, powder-free gloves when performing this procedure. Warning: To avoid eye injury, use eye protection when performing this procedure. Recommendation: Perform the sample-syringe leak test whenever you replace the sample syringe (see the console online Help).
  • Page 99 Warning: To avoid solvent spills, move the solvent bottles to a location below the sample manager. Move the solvent bottles to a location below the sample manager. Remove the knurled screw that holds the plunger of the syringe to its mounting post. Figure 3–53: Sample syringe components Syringe barrel Mounting post...

  • Page 100: Cleaning The Injection Port

    Figure 3–54: Removing sample syringe Sample-syringe valve Mounting post Syringe barrel Knurled collar Unpack the replacement sample syringe. By hand, partially fill the new syringe with purge solvent to help remove air bubbles. Requirement: Ensure that you remove all air bubbles from the syringe. 10.
  • Page 101 Warning: To avoid personal contamination with biologically hazardous or toxic compounds, wear clean, chemical-resistant, powder-free gloves when performing this procedure. Warning: To avoid eye injury, use eye protection when performing this procedure. Required tools and materials • Chemical-resistant, powder-free gloves •...
  • Page 102 Figure 3–55: Access panel captive screws Captive screws (2) Dampen a nonabrasive, lint-free wipe with LC/MS-grade water and use it to clean the areas of the injection port shaded in the image below. Figure 3–56: Injection port areas to clean In the console, select the sample manager, and then click Maintain >...
  • Page 103 Figure 3–57: Needle tip in needle guide Needle guide Needle tip 10. Allow the water and deposits to drip off the needle, and then carefully remove the wipe from beneath the needle. 11. Reinstall the access panel and use the T20 TORX driver to tighten the two screws that secure the panel to the front of the unit.

  • Page 104: Replacing The Injection-Valve Cartridge

    Seal-port tube Access-panel gap Sample-needle tubing 12. Close the sample compartment and fluidics compartment doors. 13. In the console, select the sample manager, and then click Control > Reset SM to reset the sample manager. 3.12 Replacing the injection-valve cartridge Warning: To avoid personal contamination with biologically hazardous or toxic compounds, wear clean, chemical-resistant, powder-free gloves when performing this procedure.
  • Page 105 Result: Doing so moves the needle carriage toward the back of the sample compartment. Notice: To avoid damaging electrical components and circuitry, do not disconnect an electrical assembly while electrical power is applied to a module. To completely interrupt power, set the on/off switch to the "off" position, and then disconnect the power cord from the ac source.
  • Page 106 • Orient the new cartridge exactly as the old one was oriented. • The injection-valve cartridge must slide fully into the injection-valve cartridge chamber. If it does not, report the problem to Waters Technical Service. 11. Insert the 2-mm hex screw at the 10 o’clock position on the injection-valve cartridge.

  • Page 107: Cleaning The Exterior Of The Equipment

    12. Use the 1/4-inch open-end wrench to reattach the fittings associated with the tubing for the sample needle and the seal-port tube, and tighten them as much as 1/6-turn beyond finger- tight for existing fittings, or 3/4-turn beyond finger-tight, for new fittings. 13.

  • Page 108: A Safety Advisories

    Heed all warnings when you install, repair, or operate any Waters instrument or device. Waters accepts no liability in cases of injury or property damage resulting from the failure of individuals to comply with any safety precaution when installing, repairing, or operating any of its instruments or devices.

  • Page 109: Specific Warnings

    (Risk of high-pressure gas release.) A.1.1 Specific warnings A.1.1.1 Burst warning This warning applies to Waters instruments and devices fitted with nonmetallic tubing. Warning: To avoid injury from bursting, nonmetallic tubing, heed these precautions when working in the vicinity of such tubing when it is pressurized: •...

  • Page 110: Notices

    A.1.1.2 Biohazard warning The following warning applies to Waters instruments and devices that can process biologically hazardous materials. Biologically hazardous materials are substances that contain biological agents capable of producing harmful effects in humans. Warning: To avoid infection from blood-borne pathogens, inactivated microorganisms, and other biological materials, assume that all biological fluids that you handle are infectious.

  • Page 111: Bottles Prohibited Symbol

    Use eye protection when performing this procedure. Requirement: Wear clean, chemical-resistant, powder-free gloves when performing this procedure. A.5 Warnings that apply to all Waters instruments and devices When operating this device, follow standard quality-control procedures and the equipment guidelines in this section.
  • Page 112 Advertencia: cualquier cambio o modificación efectuado en esta unidad que no haya sido expresamente aprobado por la parte responsable del cumplimiento puede anular la autorización del usuario para utilizar el equipo. 警告： 未经有关法规认证部门明确允许对本设备进行的改变或改装，可能会使使用者丧 失操作该设备的合法性。 警告： 未經有關法規認證部門允許對本設備進行的改變或修改,可能會使使用者喪失操作 該設備的權利。 경고: 규정 준수를 책임지는 당사자의 명백한 승인 없이 이 장치를 개조 또는 변경할 경우, 이...
  • Page 113 Warnung: Bei der Arbeit mit Polymerschläuchen unter Druck ist besondere Vorsicht angebracht: • In der Nähe von unter Druck stehenden Polymerschläuchen stets Schutzbrille tragen. • Alle offenen Flammen in der Nähe löschen. • Keine Schläuche verwenden, die stark geknickt oder überbeansprucht sind. •...
  • Page 114 圧力のかかったポリマーチューブを扱うときは、注意してください。 • 加圧されたポリマーチューブの付近では、必ず保護メガネを着用してください。 • 近くにある火を消してください。 • 著しく変形した、または折れ曲がったチューブは使用しないでください。 • 非金属チューブには、テトラヒドロフラン(THF)や高濃度の硝酸または硫酸などを流さないでくださ い。 • 塩化メチレンやジメチルスルホキシドは、非金属チューブの膨張を引き起こす場合があり、その場 合、チューブは極めて低い圧力で破裂します。 This warning applies to Waters instruments fitted with nonmetallic tubing. This warning applies to instruments operated with flammable solvents. June 11, 2019, 715005051 Rev. D Page 114...

  • Page 115: Warnings That Address The Replacement Of Fuses

    Warning: The user shall be made aware that if the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired. Avertissement : L’utilisateur doit être informé que si le matériel est utilisé d’une façon non spécifiée par le fabricant, la protection assurée par le matériel risque d’être défectueuses.
  • Page 116 Avertissement : pour éviter tout risque d'incendie, remplacez toujours les fusibles par d'autres du type et de la puissance indiqués sur le panneau à proximité du couvercle de la boite à fusible de l'instrument. Warnung: Zum Schutz gegen Feuer die Sicherungen nur mit Sicherungen ersetzen, deren Typ und Nennwert auf den Tafeln neben den Sicherungsabdeckungen des Geräts gedruckt sind.

  • Page 117: Electrical Symbols

    警告： 为了避免火灾，应更换“维护步骤”一章的“更换保险丝”一节中介绍的相同类 型和规格的保险丝。 警告： 為了避免火災，更換保險絲時，應使用「維護步驟」章節中「更換保險絲」所指 定之相同類型與規格的保險絲。 경고: 화재의 위험을 막으려면 유지관리 절차 단원의 “퓨즈 교체” 절에 설명된 것과 동 일한 타입 및 정격의 제품으로 퓨즈를 교체하십시오. 警告: 火災予防のために、ヒューズ交換ではメンテナンス項目の「ヒューズの交換」に記載されているタ イプおよび定格のヒューズをご使用ください。 A.7 Electrical symbols The following electrical symbols and their associated statements can appear in instrument manuals and on an instrument’s front or rear panels.

  • Page 118: Handling Symbols

    A.8 Handling symbols The following handling symbols and their associated statements can appear on labels affixed to the packaging in which instruments, devices, and component parts are shipped. Symbol Description Keep upright! Keep dry! Fragile! Use no hooks! Upper limit of temperature Lower limit of temperature Temperature limitation June 11, 2019, 715005051 Rev.

  • Page 119: B Specifications

    Specifications The reproducibility of the specifications presented in this document depends on the conditions in individual laboratories. Contact the Waters Technical Service organization for additional information about specifications. B.1 SM-FTN and bioSM-FTN physical specifications Attribute Specification Height 27.1 cm (10.7 in) with .64 cm (0.25 in) feet Width 34.3 cm (13.5 in)

  • Page 120: Sm-Ftn And Biosm-Ftn Input/Output Specifications

    Attribute Specification Overvoltage category Pollution degree Normal (IPXO) Moisture protection Line voltages, nominal Grounded AC Voltage range 100 to 240 Vac Frequency 50 to 60 Hz Maximum power draw 400 VA a. Protection Class I – The insulating scheme used in the instrument to protect from electrical shock. Class I identifies a single level of insulation between live parts (wires) and exposed conductive parts (metal panels), in which the exposed conductive parts are connected to a grounding system.
  • Page 121 • 96 and 384 microtiter plates • 48-position, 2.00-mL vial plates • 48-position, 0.65-mL micro-centrifuge tube plates • 24-position, 1.50-mL micro-centrifuge tube plates For more information, see the Waters Sample Vials and Accessories Brochure (720001818EN) or visit the plate selector vial selector on the Waters website.
  • Page 122 Item Specification Maximum sample 768 in two, 384-well Waters-certified plates, or 96 in 2-mL vial holders. capacity Four additional positions for dilution functions. For more information, see the Waters Sample Vials and Accessories Brochure, or visit the plate selector vial selector on the Waters website.
  • Page 123 • Solvent B: 100% acetonitrile • Weak wash: 10:90 acetonitrile/water • Strong wash: 10:90 acetonitrile/water • Column: ACQUITY UPLC BEH C 1.7 mm, 2.1 × 50 mm • Mobile phase: 90% solvent A:10% solvent B • Flow rate: 0.6 mL/min •...
  • Page 124 (challenge) in 10:90 acetonitrile/water +0.1% formic acid, compared with blanks of 10:90 acetonitrile/water +0.1% formic acid • Injection volume: 5 µL • Column: ACQUITY UPLC BEH C 1.7 µm, 2.1 × 50 mm • Column temperature: 40 °C • Sample temperature: 10 °C •...
  • Page 125 Figure B–1: Recommended temperature sensor locations TS Temperature sensor Figure B–2: Minimum sample compartment temperature specifications Maximum compartment temperature +4 °C Minimum sustainable compartment set point temperature -2 °C Minimum compartment temperature Ambient temperature (°C) June 11, 2019, 715005051 Rev. D Page 125...

  • Page 126: Sm-Ftn And Biosm-Ftn Wetted Materials Of Construction

    B.6 SM-FTN and bioSM-FTN wetted materials of construction Description Specification SM-FTN 316 stainless steel, DLC, gold-plated stainless steel, PEEK blend, polyimide, PPS bioSM-FTN DLC, gold-plated stainless steel, MP35N, PEEK blend, polyimide June 11, 2019, 715005051 Rev. D Page 126...

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