Mobile phase absorbance measured against air or water (Continued)
Absorbance at specified wavelength (nm)
200
EDTA,
0.11
disodium,
1 mM
HEPES, 10
2.45
mM,
pH 7.6
MES, 10
2.42
mM,
pH 6.0
Potassium
0.03
phosphate,
monobasic
(KH
PO
),
2
4
10 mM
Potassium
0.53
phosphate,
dibasic,
(K
HPO
),
2
4
10 mM
Sodium
1.85
acetate,
10 mM
Sodium
2.00
chloride,
1 M
Sodium
2.48
citrate,
10 mM
Sodium
1.00
formate,
10 mM
205
210
215
0.07
0.06
0.04
2.50
2.37
2.08
2.38
1.89
0.90
<0.01 —
—
0.16
0.05
0.01
0.96
0.52
0.30
1.67
0.40
0.10
2.84
2.31
2.02
0.73
0.53
0.33
220
230
240
0.03
0.03
0.02
1.50
0.29
0.03
0.45
0.06
<0.01 —
—
—
—
<0.01 —
—
0.15
0.03
<0.01 —
<0.01 —
—
1.49
0.54
0.12
0.20
0.03
<0.01 —
Mobile phase absorbance
250
260
280
0.02
0.02
0.02
<0.01 —
—
—
—
—
—
—
—
—
—
—
—
—
—
—
0.03
0.02
0.01
—
—
107