Waters ACQUITY UPLC Operator's, Overview And Maintenance Manual page 50

• To conserve lamp life, extinguish the lamp while leaving the detector
running but idle. Note, however, that you should do so only when the
lamp will remain extinguished more than 4 hours.
• If you use buffered mobile phase, flush it from the detector before
powering-off to prevent these adverse conditions:
– Plugging of solvent lines and the flow cell
– Damaging of instrument components
– Microbial growth
Caution:
• To ensure optimum performance of the light-guiding flow cell, ensure
that eluent is flowing prior to powering-on the detector. If, however,
you must power-on the detector before the eluent is flowing,
extinguish the lamp after establishing communications.
• If the light-guiding flow cell will not be used for a period of time, flush
it with clean mobile phase, such as a water/acetonitrile or
water/methanol mix, and either cap the flow ports or dry the flow cell
with pure nitrogen or pure helium for 5 to 10 minutes.
• To avoid damaging the detector or column, remove the column and
disconnect the detector before you flush the system.
Flushing the detector
To flush the detector
1. Remove the column from the system.
2. Flush the system to waste with 100% HPLC-quality water at a rate of
1.0 ml/minute for 10 minutes.
3. Flush the system with a solution of 90:10 methanol/water for 10
minutes.
38
To avoid damaging the detector, do not exceed the
Caution:
6895 kPa (69 bar, 1000 psi) pressure limitation of the flow cell.