Waters ACQUITY UPLC Operator's, Overview And Maintenance Manual page 116

Mixed mobile phases
The table below contains approximate wavelength cutoffs for some other
solvents, buffers, detergents, and mobile phases. The solvent concentrations
represented are those most commonly used. If you want to use a different
concentration, you can determine approximate absorbance using Beer's Law,
because absorbance is proportional to concentration.
Wavelength cutoffs for different mobile phases
Mobile phase
Acetic acid, 1%
Ammonium acetate,
10 mM
Ammonium bicarbonate,
10 mM
BRIJ 35, 0.1%
CHAPS, 0.1%
Diammonium phosphate,
50 mM
EDTA, disodium, 1 mM
HEPES, 10 mM, pH 7.6
Hydrochloric acid, 0.1%
MES, 10 mM, pH 6.0
Potassium phosphate,
monobasic, 10 mM
dibasic, 10 mM
Sodium acetate, 10 mM
104
UV
cutoff Mobile phase
(nm)
230 Sodium chloride, 1 M
205 Sodium citrate, 10 mM
190 Sodium dodecyl sulfate
190 Sodium formate, 10 mM
215 Triethyl amine, 1%
205 Trifluoracetic acid, 0.1%
190 TRIS HCl, 20 mM, pH 7.0,
pH 8.0
225 Triton-X™ 100, 0.1%
190
Waters PIC
1 vial/liter
215 Waters PIC Reagent B-6,
1 vial/liter
Waters PIC Reagent B-6,
190 low UV, 1 vial/liter
190
205 Waters PIC Reagent D-4,
1 vial/liter
UV
cutoff
(nm)
207
225
190
200
235
190
202, 212
240
200
®
Reagent A,
225
190
190