Waters ACQUITY UPLC Operator's, Overview And Maintenance Manual page 60

Photodiode array and egphotodiode array detector
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flush (see
"Performing a system, acid-cleansing flush" on page
observe no improvement, call Waters Technical Service.
Caution:
that can create backpressure exceeding the flow cell's maximum rating
of 6895 kPa (69 bar, 1000 psi).
The pressure through the flow cell must not exceed 6895 kPa (69 bar,
1000 psi). Increasing the flow rate usually increases the pressure.
High-viscosity fluids generally increase the pressure through the flow
cell and therefore require a lower flow rate. Allowable flow rates are
based on the limit of pressure that each flow cell can withstand.
Warning:
intervals.
Always use clean, well-degassed eluents.
Rule:
Required materials
1% formic acid
Clean, chemical-resistant, powder-free gloves
Water (for flushing buffers)
Intermediate solvent that is miscible in both the mobile phase and water
Stainless steel unions (to replace the column during flushing)
Wrench suitable for removing and replacing the column
To clean the flow cell
1.
In the detector control panel, click
2.
Stop the solvent flow and remove the column.
3.
Replace the column with a union or piece of tubing.
48
To prevent flow cell failure, do not connect any tubing or device
To avoid spills, empty the waste container at regular
(Lamp Off).
50). If you still

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