Waters ACQUITY UPLC Operator's, Overview And Maintenance Manual page 42

Result:
tests.
The power and lamp LEDs change as follows:
• Each system instrument's power LED shows green.
• During initialization, each system instrument's status LED flashes
green.
• After the instruments are successfully powered-on, all LEDs show
steady green. The solvent manager's flow LED and the sample
manager's run LED remain unlit.
3. Start Empower, MassLynx, or a third-party software. You can monitor
the ACQUITY console for messages and LED indications.
4. Flush the system with filtered, degassed, and sparged HPLC-grade
methanol or acetonitrile.
5. In the console, set the solvent manager to deliver a flow rate appropriate
for the flow cell in your system.
Use only thoroughly degassed HPLC-grade solvents. Gas in the
Tip:
mobile phase can form bubbles in the flow cell and cause the detector to
fail the Reference Energy diagnostic test.
6. Pump mobile phase for at least 15 minutes.
7. Ensure the detector cell is filled with solvent and free of bubbles.
8. Press the power switch on the front panel to power-on the detector.
Result:
lamp LED blinks green. The lamp LED shows steady green when the
lamp is ignited.
9. When the lamp LED is steady green, start Empower, MassLynx, or a
third-party software, and download an instrument or inlet method.
Result:
10. For best results, wait one hour for the detector to stabilize before
acquiring data.
30
Each system instrument "beeps" and runs a series of startup
The detector can fail to initialize correctly if the cell
Caution:
contains air. To avoid damaging the light-guiding flow cell, do not
ignite the detector lamp when no solvent is flowing through the
cell or when it is dry.
The detector runs a series of startup diagnostic tests while the
The ACQUITY Console displays messages and visual signals.