Waters ACQUITY Overview And Maintenance Manual
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Waters ACQUITY Overview And Maintenance Manual

Qda detector
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ACQUITY QDa Detector
Overview and Maintenance Guide
715003956
Copyright © Waters Corporation 2017
Revision F
All rights reserved

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  • Page 1 ACQUITY QDa Detector Overview and Maintenance Guide 715003956 Copyright © Waters Corporation 2017 Revision F All rights reserved...

  • Page 2: General Information

    This document is believed to be complete and accurate at the time of publication. In no event shall Waters Corporation be liable for incidental or consequential damages in connection with, or arising from, its use. For the most recent revision of this document, consult the Waters website (waters.com).

  • Page 3: Customer Comments

    Contacting Waters Contact Waters with enhancement requests or technical questions regarding the use, transportation, removal, or disposal of any Waters product. You can reach us via the Internet, telephone, fax, or conventional mail. Waters contact information Contacting medium Information...

  • Page 4: Safety Hazard Symbol Notice

    Waters distributor. Solvent leakage hazard The source exhaust system is designed to be robust and leak-tight. Waters recommends that you perform a hazard analysis, assuming a maximum leak into the laboratory atmosphere of 10% LC eluate. If the instrument is a standard QDa fitted with a diaphragm pump, assume an additional 0.5% maximum leak into the laboratory atmosphere.
  • Page 5 High temperature hazard Warning: To avoid burn injuries, exercise care when handling the components of the source enclosure heated to high temperatures. Wait until the hot components have sufficiently cooled before you handle them. Mass spectrometer high temperature hazard Source enclosure Source components High-voltage hazard Warning:...

  • Page 6: Electrical Power Safety Notice

    Do not dispose of the instrument or return it to Waters for repair until the authority responsible for approving its removal from the premises specifies the extent of decontamination required and the level of residual contamination permissible.

  • Page 7: Equipment Misuse Notice

    Equipment misuse notice If equipment is used in a manner not specified by its manufacturer, protections against personal injury inherent in the equipment’s design can be rendered ineffective. Safety advisories Consult the "Safety advisories" appendix in this publication for a comprehensive list of warning advisories and notices.

  • Page 8: Audience And Purpose

    Waters designed the ACQUITY QDa detector for use as an ion confirmation and quantitation tool, as part of an ACQUITY UPLC or UPC² system, Alliance, and LC and SFC prep systems. The ACQUITY QDa detector is not intended for use in diagnostic applications.

  • Page 9: Emc Considerations

    EMC considerations FCC radiation emissions notice Changes or modifications not expressly approved by the party responsible for compliance, could void the user's authority to operate the equipment. This device complies with Part 15 of the FCC Rules. Operation is subject to the following two conditions: (1) this device may not cause harmful interference, and (2) this device must accept any interference received, including interference that may cause undesired operation.

  • Page 10: Ec Authorized Representative

    EC authorized representative Address Waters Corporation Stamford Avenue Altrincham Road Wilmslow SK9 4AX UK Telephone +44-161-946-2400 +44-161-946-2480 Contact Quality manager June 15, 2017, 715003956 Rev. F Page x...

  • Page 11: Table Of Contents

    Equipment misuse notice .......................vii Safety advisories ..........................vii Operating this device ..........................vii Applicable symbols.........................vii Audience and purpose........................viii Intended use of the ACQUITY QDa detector ................viii Calibrating .............................viii Quality control..........................viii EMC considerations..........................ix FCC radiation emissions notice...................... ix Canada spectrum management emissions notice................ix ISM classification: ISM group 1 class A..................
  • Page 12 1.6 Identifying your instrument version ....................17 1.7 ACQUITY Diverter Valve....................... 19 2 Preparing for operation....................20 2.1 Rear panel connections ........................ 20 2.2 Connecting to the electricity source ....................20 2.3 Connecting the probe........................21 2.4 Starting the instrument ........................22 2.5 Stop flow ............................23 3 Maintenance procedures ..................25 3.1 Maintenance schedule ........................
  • Page 13 A.1.1 Specific warnings ........................ 60 A.2 Notices............................62 A.3 Bottles Prohibited symbol ......................62 A.4 Required protection ........................63 A.5 Warnings that apply to all Waters instruments and devices ............63 A.6 Warnings that address the replacing of fuses................67 A.7 Electrical and handling symbols ....................68 A.7.1 Electrical symbols........................ 68 A.7.2 Handling symbols ........................
  • Page 14 C.2 Running Hexane and THF with the ACQUITY QDa Detector ............74 C.3 QDa solvent compatibility (KAD) ....................75 C.3.1 Items exposed to solvent ....................75 C.3.2 Solvents used to prepare mobile phases ................76 C.3.3 Solvents used infrequently ....................77 C.3.4 Solvents used commonly as diluents .................. 78 C.4 ...
  • Page 15 D.12 Connecting to the power supply ....................114 E Optional ACQUITY Diverter Valve ................115 E.1 Diverter valve safety advisories ....................115 E.2 Installing the ACQUITY Diverter Valve ..................115 E.2.1 Fitting the diverter valve assembly ..................115 E.2.2 Diverter valve wiring ......................120 E.2.3 Configuring the diverter valve fluidics ................122 E.2.4 Configuring the diverter valve waste tubing ..............

  • Page 16: Instrument Features And Operating Modes

    Instrument features and operating modes To effectively use the ACQUITY QDa Detector, you must familiarize yourself with its features and operating modes. 1.1 Operating modes You can acquire data using any of these operating modes: • Scanning, where the instrument scans across a user-defined span of mass-to-charge (m/z) ratios to produce a mass spectrum.

  • Page 17: Sample Inlet

    To learn how to enable start-up checks, see the instrument’s online Help. 1.4 Sample inlet You can introduce sample into the QDa detector from an ACQUITY LC system or from a syringe pump. If you are using an LC system, connect the ACQUITY QDa detector to the preceding instrument in your system using the supplied probe assembly.
  • Page 18 Table 1–1: Instrument versions Serial number suffix Models Backing pump Source exhaust Performance Diaphragm (attached) Internal valve Standard Rotary (external) Performance Diaphragm (attached) External valve assembly Standard Rotary (external) Figure 1–1: QDa with internal source exhaust (KAD version) QDa with internal source exhaust (KAD version) Maintenance Procedures External Connections June 15, 2017, 715003956 Rev.

  • Page 19: Acquity Diverter Valve

    External Connections 1.7 ACQUITY Diverter Valve The ACQUITY Diverter Valve is supplied as an optional accessory to the ACQUITY QDa Detector. The diverter valve is used to divert column eluent from the QDa to waste when handling variable samples and solutions, and can also support sample loop injections. You can attach the diverter valve to the QDa instrument using the bracket assembly supplied with the diverter valve kit.

  • Page 20: Preparing For Operation

    2.1 Rear panel connections When making connections to the QDa detector’s rear panel, refer to the figure in Appendix When making connections between the QDa detector’s rear panel and the ACQUITY Diverter Valve's rear panel, refer to the figure in Diverter valve wiring.

  • Page 21: Connecting The Probe

    on the back of the cart. Finally, connect the power strip’s cable to a wall outlet operating on its own circuit. 2.3 Connecting the probe To avoid puncture injuries from the sharp probe capillary, use care when inserting it into the source enclosure.

  • Page 22: Starting The Instrument

    Figure 2–1: Inserting the probe PEEK fitting Probe assembly Probe fitting Capillary Secure the probe tubing to the side of the source enclosure using the clip. 2.4 Starting the instrument Warning: To avoid injury from electrical shock or fire, and damage to the equipment, follow these guidelines: •...

  • Page 23: Stop Flow

    In the software, monitor the Instrument Console for messages and LED indications. 2.5 Stop flow When the ACQUITY QDa Detector is switched into Standby mode, or a nitrogen gas failure is detected, the instrument sends a stop-flow signal to the LC. The stop-flow signal switches off all solvent flow from the LC in order to prevent damage to the ACQUITY QDa Detector.
  • Page 24 If the LC flow needs to be reestablished while the ACQUITY QDa Detector is in Standby mode, or when it is switched off, you must ensure that the instrument is removed from the solvent flow path. Notice: To avoid irreparable damage to the instrument when it is in Standby mode or switched off, disconnect the instrument from the solvent flow path.

  • Page 25: Maintenance Procedures

    Maintenance procedures This section provides the maintenance guidelines and procedures necessary to maintain the device's performance. Keep to a maintenance schedule, and perform maintenance as required and described in this section. 3.1 Maintenance schedule The following table lists periodic maintenance schedules that ensure optimum instrument performance.

  • Page 26: Spare Parts

    3.2 Spare parts To ensure that your system operates as designed, use only Waters Quality Parts. Visit www.waters.com/wqp for information about Waters Quality Parts, including how to order them. 3.3 Replacing fuses...

  • Page 27: Troubleshooting With Connections Insight

    Connections INSIGHT is an intelligent device management (IDM) Web service that enables Waters to provide proactive service and support for a system. To use Connections INSIGHT, you must install its service agent software on the same PC as the chromatography data software. In a client/server system, you must also install the service agent on the computer that controls the system.

  • Page 28: Removing And Refitting The Source Enclosure

    Warning: To avoid personal contamination with biohazardous or toxic compounds, wear clean, chemical-resistant, powder-free gloves when performing this procedure. Warning: To prevent personal injury, always observe Good Laboratory Practice when handling solvents, changing tubing, or operating the detector. Know the physical and chemical properties of the solvents used (see the Material Safety Data Sheets for the solvents in use).
  • Page 29 Required materials • Chemical-resistant, powder-free gloves To remove the source enclosure: Power-off the instrument using the power button in the top left-hand corner of the front panel. Wait approximately 5 minutes to allow the instrument to vent. Open the source enclosure door. Disconnect the source enclosure's electrical cable from the front of the instrument by loosening the screws and pull the cable from the socket.

  • Page 30: Fitting The Source Enclosure To The Instrument

    Probe entrance Cable screws Source enclosure thumbscrew Source enclosure thumbscrew Remove the source enclosure by pulling it away from the instrument using both hands. Warning: To avoid puncture injuries from the sharp probe tip, use care when inserting and removing the probe from the source enclosure. Remove the probe from the top of the source enclosure using the probe fitting (see the figure "Inserting the probe"...

  • Page 31: Maintaining The Source Components

    3.8 Maintaining the source components Clean the source components when these conditions apply: • The sample cone and cone gas nozzle are visibly fouled. • You have dismissed inlet and sample-related causes for decreased signal intensity. 3.8.1 Removing the sample cone assembly from the ion block Required materials •...
  • Page 32 Figure 3–2: Removing the gas cone and PEEK cone clamp PEEK cone clamp Gas cone Ion block Remove the sample cone assembly from the right-hand side of the ion block and disassemble the parts by hand. Note: • Three parts compose the sample cone assembly: a metal sample cone, a disc aperture assembly, and a metal entrance-aperture carrier.
  • Page 33 Figure 3–3: Removing the sample cone assembly Calibration pin Sample cone assembly Ion block Figure 3–4: Sample cone assembly components June 15, 2017, 715003956 Rev. F Page 33...

  • Page 34: Replacing The Entrance-Aperture Seal And Disc

    Entrance-aperture carrier Disc aperture assembly Sample cone 3.8.2 Replacing the entrance-aperture seal and disc Replace the entrance seal aperture when these conditions apply: • You have dismissed LC and sample-related causes for decreased signal intensity. • Cleaning the source components fails to increase signal stability. Required materials •...
  • Page 35 Warning: To avoid personal contamination with biohazardous or toxic compounds, wear clean, chemical-resistant, powder-free gloves when performing this procedure. Warning: To avoid puncture injuries from the sharp calibration pin, use care when removing it from and replacing it in the ion block. To remove the ion block: Insert a flat-blade screwdriver beneath the top of the calibration pin, and then lever the pin away from the ion block.

  • Page 36: Cleaning The Source Components

    Securing screws Ion block If your ion block includes the slot on the right-hand side near the top, insert the short end of the hex wrench and, using the long end, lever the wrench from side to side to break the seal between the ion block and the source housing (see the figure below).
  • Page 37 • Formic acid • Ultrasonic bath • Source of oil-free, inert gas (nitrogen or argon) for drying (air-drying optional) • Wash bottle containing HPLC-grade (or better) 1:1 methanol/water • Large beaker To clean the sample cone and cone gas nozzle: Warning: To avoid personal contamination with biohazardous or toxic compounds, wear clean, chemical-resistant, powder-free gloves when performing this procedure.

  • Page 38: Refitting The Source Components

    Note: Do not clean the consumable components of the aperture assembly, such as the entrance-aperture seal and entrance-aperture disc. Instead, replace these consumables when performing general maintenance, the components are visibly damaged, or the performance or cleanliness of the machine is compromised. Tip: If the components are obviously contaminated, use 45:45:10 methanol/water/formic acid.

  • Page 39: Cleaning The Instrument Case

    If you removed the O-rings from the source components, refit them. Insert the rubber entrance-aperture seal into the sample cone. Insert the entrance-aperture carrier into the sample cone assembly. Note: Ensure that the entrance aperture seal is in place in the entrance-aperture carrier. Insert the entrance-aperture carrier and sample cone assembly into the entrance-aperture on the right-hand side of the ion block.
  • Page 40 Warning: To avoid personal contamination with biohazardous or toxic compounds, wear clean, chemical-resistant, powder-free gloves when performing this procedure. Warning: To avoid puncture injuries from the sharp calibration pin, use care when removing it from and replacing it in the ion block. Warning: To avoid burn injuries, exercise care when handling the components of the source enclosure heated to high temperatures.
  • Page 41 Figure 3–8: Removing the pumping block and ion guide Securing screws Pumping block Remove the 3 slotted screws securing the differential aperture to the ion guide housing using the flat-blade screwdriver, and then remove the differential aperture (see the figure "Removing the differential aperture"...
  • Page 42 Figure 3–9: Removing the differential aperture Electrical connectors Ion guide support rods Differential aperture support Differential aperture support screws O-ring Differential aperture securing screws Differential aperture Ion guide Disconnect the 4 electrical connectors from the ion guide, leaving them attached to the pumping block.
  • Page 43 Figure 3–10: Ion guide – electrical connector Ion guide electrical connectors – top view Ion guide electrical connectors – underside view Remove the 2 screws securing the differential aperture support to the ion guide support rods using the 3-mm hex wrench, and then remove the differential aperture support and ion guide from the pumping block.

  • Page 44: Cleaning The Differential Aperture

    To clean the differential aperture: Place the differential aperture in the glass vessel. Add Waters MS Cleaning Solution or 1:1 methanol/water to the vessel until the differential aperture is immersed completely. Place the vessel containing the differential aperture in the ultrasonic bath for 20 minutes.

  • Page 45: Cleaning The Ion Guide Assembly

    • Two lengths of PEEK, PTFE, or stainless steel tubing, appropriately sized for suspending the ion guide assembly in the glass vessels when cleaning • HPLC-grade deionized water • Waters MS Cleaning Solution (186006846) or HPLC-grade (or better) 1:1 methanol/water • Holding container for used Waters MS Cleaning Solution • HPLC-grade isopropyl alcohol •...
  • Page 46 Figure 3–12: Cleaning the ion guide Ion guide Add Waters MS Cleaning Solution or 1:1 methanol/water to the glass vessel until the ion guide is immersed completely. Place the vessel containing the ion guide in the ultrasonic bath for 20 minutes.

  • Page 47: Fitting The Ion Guide Assembly To The Instrument

    3.10.4 Fitting the ion guide assembly to the instrument Required materials • Chemical-resistant, powder-free gloves • 2.5-mm hex wrench Warning: To avoid personal contamination with biohazardous or toxic compounds, wear clean, chemical-resistant, powder-free gloves when performing this procedure. To fit the ion guide assembly to the instrument: Fit the differential aperture O-ring to the differential aperture support (see the figure "Removing the pumping block and ion guide"...
  • Page 48 Tip: It is normal for the ion guide assembly to have some freedom to move when assembled correctly. Carefully slide the pumping block and ion guide assembly into the instrument’s source housing. Tip: To correctly orientate the pumping block, ensure that the ion guide remains visible at the left-hand edge of the pumping block’s front face.

  • Page 49: Emptying The Nitrogen Exhaust Trap

    Securing screws Pumping block Fit the ion block and source components to the instrument (see Refitting the source components). Fit the source enclosure to the instrument (see Fitting the source enclosure to the instrument). 3.11 Emptying the nitrogen exhaust trap Inspect the nitrogen exhaust trap in the instrument’s exhaust line daily, and empty it before it is more than 10% full.
  • Page 50 Figure 3–15: Nitrogen exhaust trap From instrument exhaust connection (12-mm OD) To laboratory exhaust port (12-mm O.D.) Exhaust solenoid cable Required materials • Chemical-resistant, powder-free gloves To empty the trap: Requirement: Wear clean, chemical-resistant, powder-free gloves when performing this procedure. In the software, stop the LC flow.

  • Page 51: Emptying The Liquid-Trap Bottle

    Unscrew and remove the nitrogen exhaust trap from its cap and associated fittings. Warning: To avoid spreading contamination with biologically hazardous, toxic, and corrosive materials, dispose of all waste materials according to local environmental regulations. Dispose of the waste liquid in accordance with local environmental regulations. Fit and fully tighten the trap onto its cap.
  • Page 52 Warning: To avoid personal contamination with biohazardous or toxic compounds, wear clean, chemical-resistant, powder-free gloves when performing this procedure. Warning: To avoid burn injuries, allow the pump to cool before touching surfaces displaying the burn warning symbol. To add oil to the backing pump: Power-off the instrument using the power button in the top, left-hand corner of the front panel.

  • Page 53: Gas Ballasting The Rotary Backing Pump

    Oil level sight glass Oil drain plug Tilt the pump slightly and catch the oil in a suitable container. Dispose of the oil according to local environmental regulations. Insert the oil-drain plug into the pump’s rear panel. To flush the pump, pour 50-mL of fresh oil into the pump inlet, on the pump’s top side. Tip: If you encounter difficulty, remove the separator.

  • Page 54: Replacing The Internal Source Exhaust Valve (Kad)

    For information about replacing the backing pump’s demister element, see “Troubleshooting” in the VACUUBRAND Technology for Vacuum Systems Instructions for use (VACUUBRAND part number 99139 / 11/20/2013). Contact Waters to order parts (see Contacting Waters). 3.16 Replacing the internal source exhaust valve (KAD) Replace the source exhaust valve annually, or when a source-pressure test fails and all other causes of failure have been investigated.

  • Page 55: Removing The Source Exhaust Valve

    Exhaust trap bottle Source exhaust tube elbow connector Waste liquid drain port 3.16.1 Removing the source exhaust valve Before removing the source exhaust valve, ensure that a replacement valve (289010426) is available to install. Required materials • Chemical-resistant, powder-free gloves •...

  • Page 56: Fitting The Source Exhaust Valve

    Figure 3–18: Removing the source exhaust valve Source valve enclosure Alignment key and grip panel keyway Valve grip panel Retaining screws Source exhaust valve Source exhaust tube elbow connector Dispose of the source exhaust valve in accordance with local environmental regulations. 3.16.2 Fitting the source exhaust valve Perform the following procedure to fit the source exhaust valve into the enclosure.
  • Page 57 To insert the source exhaust valve into the enclosure: Requirement: Wear clean, chemical-resistant, powder-free gloves when performing this procedure. To gain access for inserting the valve into the enclosure, remove any obstructive exhaust tubing and rotate the elbow connector of the source exhaust tube clear of the path. Hold the source exhaust valve by the grip panel, orienting the curved edge of the panel toward the left.
  • Page 58 Ensure that the valve is firmly in place so that the panel is flush with the outer edge of the enclosure. Insert the 4 retaining screws in each corner of the grip panel and tighten them using the 5- mm hex wrench. Reconnect the source exhaust by threading the tubing through the hose brackets alongside the instrument and inserting the tubing into the elbow connector.

  • Page 59: A Safety Advisories

    Heed all warnings when you install, repair, or operate any Waters instrument or device. Waters accepts no liability in cases of injury or property damage resulting from the failure of individuals to comply with any safety precaution when installing, repairing, or operating any of its instruments or devices.

  • Page 60: Specific Warnings

    The following warnings (both symbols and text) can appear in the user manuals of particular instruments and devices, and on labels affixed to them or their component parts. A.1.1.1 Burst warning This warning applies to Waters instruments and devices fitted with nonmetallic tubing. June 15, 2017, 715003956 Rev. F Page 60...
  • Page 61 • The nitrogen supply pressure does not fall below 600 kPa (6 bar, 87 psi) during an analysis requiring the use of flammable solvents. A.1.1.4 Biohazard warning The following warning applies to Waters instruments and devices that can process material containing biohazards, which are substances that contain biological agents capable of producing harmful effects in humans.

  • Page 62: Notices

    A.1.1.5 Biohazard and chemical hazard warning This warning applies to Waters instruments and devices that can process biohazards, corrosive materials, or toxic materials. Warning: To avoid personal contamination with biohazardous, toxic, or corrosive materials, you must understand the hazards associated with their handling.

  • Page 63: Required Protection

    Use eye protection when performing this procedure. Requirement: Wear clean, chemical-resistant, powder-free gloves when performing this procedure. A.5 Warnings that apply to all Waters instruments and devices When operating this device, follow standard quality-control procedures and the equipment guidelines in this section.
  • Page 64 警告: 規制機関から明確な承認を受けずに本装置の変更や改造を行うと、本装置のユーザーとして の承認が無効になる可能性があります。 Warning: Use caution when working with any polymer tubing under pressure: • Always wear eye protection when near pressurized polymer tubing. • Extinguish all nearby flames. • Do not use tubing that has been severely stressed or kinked. •...
  • Page 65 Avvertenza: fare attenzione quando si utilizzano tubi in materiale polimerico sotto pressione: • Indossare sempre occhiali da lavoro protettivi nei pressi di tubi di polimero pressurizzati. • Spegnere tutte le fiamme vive nell'ambiente circostante. • Non utilizzare tubi eccessivamente logorati o piegati. •...
  • Page 66 い。 • 塩化メチレンやジメチルスルホキシドは、非金属チューブの膨張を引き起こす場合があり、その場 合、チューブは極めて低い圧力で破裂します。 This warning applies to Waters instruments fitted with nonmetallic tubing. This warning applies to instruments operated with flammable solvents. Warning: The user shall be made aware that if the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired.

  • Page 67: Warnings That Address The Replacing Of Fuses

    警告： 使用者必须非常清楚如果设备不是按照制造厂商指定的方式使用，那么该设备所 提供的保护将被削弱。 경고: 제조업체가 명시하지 않은 방식으로 장비를 사용할 경우 장비가 제공하는 보호 수단이 제대로 작동하지 않을 수 있다는 점을 사용자에게 반드시 인식시켜야 합니다. 警告: ユーザーは、製造元により指定されていない方法で機器を使用すると、機器が提供している 保証が無効になる可能性があることに注意して下さい。 A.6 Warnings that address the replacing of fuses The following warnings pertain to instruments and devices equipped with user-replaceable fuses. Information describing fuse types and ratings sometimes, but not always, appears on the instrument or device.

  • Page 68: Electrical And Handling Symbols

    警告: 火災予防のために、ヒューズ交換では機器ヒューズカバー脇のパネルに記載されているタイプお よび定格のヒューズをご使用ください。 Finding fuse types and ratings when that information does not appear on the instrument or device: Warning: To protect against fire, replace fuses with those of the type and rating indicated in the “Replacing fuses” section of the Maintenance Procedures chapter. Avertissement : pour éviter tout risque d'incendie, remplacez toujours les fusibles par d'autres du type et de la puissance indiqués dans la rubrique "Remplacement des...

  • Page 69: Handling Symbols

    Symbol Description Electrical power on Electrical power off Standby Direct current Alternating current Alternating current (3 phase) Safety ground Frame, or chassis, terminal Fuse Functional ground Input Output A.7.2 Handling symbols The following handling symbols and their associated statements can appear on labels affixed to the packaging in which instruments, devices, and component parts are shipped.
  • Page 70 Symbol Description Use no hooks! Upper limit of temperature Lower limit of temperature Temperature limitation June 15, 2017, 715003956 Rev. F Page 70...

  • Page 71: B Specifications

    Specifications The applicability of the following specifications depends on the conditions in individual laboratories. Refer to the ACQUITY QDa Detector System Site Preparation Guide, or contact the Waters Technical Service organization for additional information about the specifications. B.1 Physical specifications The following table lists the physical specifications for the ACQUITY QDa detector.

  • Page 72: Input/Output Specifications

    Moisture Protection – Normal (IPX0) – IPX0 means that no Ingress Protection against any type of dripping or sprayed water exists. The “X” is a placeholder that identifies protection against dust, if applicable. B.4 Input/output specifications The following table lists the input and output specifications for the ACQUITY QDa detector. Table B–4: Input and output specifications (Continued) Attribute...
  • Page 73 Table B–4: Input and output specifications (Continued) (continued) Attribute Specification Analog Out Maximum output: +10 V Minimum output: -10 V Output current: 10 mA June 15, 2017, 715003956 Rev. F Page 73...

  • Page 74: C Materials Of Construction And Compatible Solvents

    The lists of solvents presented in this appendix are not comprehensive. Some solvents might be chemically compatible with the ACQUITY QDa detector, though they are not referenced in the lists. Some solvents referenced in the lists but used at higher concentrations than specified might also be compatible with the instrument.

  • Page 75: Qda Solvent Compatibility (Kad)

    C.3 QDa solvent compatibility (KAD) Note: The solvent compatibility information in this section refers to the KAD version of the QDa detector, shown in the following figure: Figure C–1: QDa detector (KAD) PTFE exhaust tubing Exhaust trap bottle Source exhaust tube elbow connector Waste liquid drip port C.3.1 Items exposed to solvent The items that appear in the following table can be exposed to solvent.

  • Page 76: Solvents Used To Prepare Mobile Phases

    Table C–1: Items exposed to solvent (KAD) Item Material Gas exhaust port Aluminum Gas tubes FEP (fluorinated ethylene propylene) Ion block Stainless steel Ion block support PEEK (polyetheretherketone) O-rings Viton or PTFE (polytetrafluoroethylene)-encapsulated Viton Solvent waste/leak management Fluorinated ethyline polymer (FEP) tubing Source enclosure Aluminum Waste bottle...

  • Page 77: Solvents Used Infrequently

    Table C–2: Solvents used to prepare mobile phases (KAD) (continued) Solution Concentration Ethanol 100% Isopropanol 100% Propanol 100% Formic acid ≤0.5% Acetic acid ≤1% Trifluoroacetic acid (TFA) ≤0.2% Ammonium hydroxide ≤1% Ammonium formate ≤50 mM Ammonium acetate ≤50 mM Ammonium bicarbonate ≤50 mM Tetrahydrofuran (THF) ≤15%...

  • Page 78: Solvents Used Commonly As Diluents

    C.3.4 Solvents used commonly as diluents The following solvents, often used as sample diluents, are not expected to adversely affect the performance of the materials shown in the list of items exposed to solvents in the table "Items exposed to solvent (KAD)" in the topic Items exposed to solvent.

  • Page 79: Items Exposed To Solvent

    Exhaust tubing API gas connection Source exhaust connection Holding screws Convoluted liquid waste tubing Blanking fitting Unused source exhaust connection Note: The assembly cover is depicted as transparent to reveal the tubing connections. C.4.1 Items exposed to solvent The items that appear in the following table can be exposed to solvent. You must evaluate the compatibility issues if the solvents used in your application differ from the solvents usually used with these items.

  • Page 80: Solvents Used To Prepare Mobile Phases

    • The solvents shown in the table below do not comprise a comprehensive listing. Some solvents might be chemically compatible with the ACQUITY QDa detector, though they are not referenced in the lists. Some solvents that are referenced in the lists, but used at higher concentrations than specified, might also be compatible with the instrument.
  • Page 81 June 15, 2017, 715003956 Rev. F Page 81...

  • Page 82: D External Connections

    Notice: To avoid damaging system modules, contact Waters Technical Service before moving the modules. If you must transport a system component or remove it from service, contact Waters Technical Service for recommended cleaning, flushing, and packaging procedures.
  • Page 83 Vacuum pump tubing Nitrogen inlet Diaphragm pump securing screw Diaphragm pump supports Diaphragm pump securing screw Waste bottle solenoid connection Power cable For details of supported system configurations, contact Waters Technical Service. June 15, 2017, 715003956 Rev. F Page 83...

  • Page 84: Connecting The Standard Instrument's Backing Pump

    D.2 Connecting the Standard instrument’s backing pump The Standard instrument uses a diaphragm pump attached to the back of the instrument. Note: To connect the Performance instrument’s backing pump, see Connecting the Performance instrument’s backing pump. Required materials • Chemical-resistant, powder-free gloves •...

  • Page 85: Connecting The Performance Instrument's Backing Pump

    D.3 Connecting the Performance instrument’s backing pump Note: To connect the Standard instrument’s backing pump, see Connecting the Standard instrument’s backing pump. Figure D–2: Connecting the backing pump Vacuum hose assembly Exhaust port Power cable June 15, 2017, 715003956 Rev. F Page 85...
  • Page 86 Required materials • Chemical-resistant, powder-free gloves • 7-mm nut driver • 8-mm hex wrench • Utility knife • NW16 clamp (included in the installation kit) • NW16 O-ring (included in the installation kit) • PVC exhaust tubing (included in the installation kit) •...
  • Page 87 Figure D–3: Backing pump clearance Left-side minimum clearance is 15.24 cm (6 inches) Back-side minimum clearance is 15.24 cm (6 inches) Right-side minimum clearance is 15.24 cm (6 inches) Front-side minimum clearance is 35.56 cm (14 inches) To connect the backing pump: Place a suitable PTFE drip tray on the floor within 5 feet of the instrument.
  • Page 88 Use the NW16 center ring and clamp and the 7-mm nut driver to attach the flanged end of a length of 1-inch ID vacuum hose to the vacuum port on the pump. Use a hose clamp to connect the opposite end of the length of 1-inch vacuum hose in step 3 to the 1-inch OD straight vacuum port on the instrument’s rear panel.

  • Page 89: Making The Electrical Connections To The Rotary Backing Pump

    D.3.1 Making the electrical connections to the rotary backing pump Figure D–4: Backing pump electrical connections To power source Backing pump connector Pump control cable June 15, 2017, 715003956 Rev. F Page 89...

  • Page 90: Connecting To The Nitrogen Gas Supply

    Pump switching box Power cable To make the electrical connections to the rotary backing pump: Connect the pump control cable from the pump switching box to the backing pump connector on the instrument’s rear panel. Connect the rotary backing pump power cord to the pump switching box. Connect the pump switching box power cord to the main power source and use the power switch on the pump to power it on.

  • Page 91: Connecting And Disconnecting The External Source Exhaust Valve Assembly (Kab)

    Figure D–5: 6-mm stud Connect the free end of the long piece of 6-mm PTFE tubing to the 6-mm stud. Requirement: The nitrogen must be dry and oil-free, with a purity of at least 95%. Regulate the supply at 600 to 690 kPa (6.0 to 6.9 bar, 87 to 100 psi). D.5 Connecting and disconnecting the external source exhaust valve assembly (KAB) Replace the source exhaust valve annually, or when a source pressure test fails and all other...
  • Page 92 Connect the valve assembly to the instrument’s push-in source exhaust outlet (for more information, see the figure "External source exhaust valve assembly—rear configuration" on step 4). Connect the waste tubing to the valve assembly as follows: • Connect the API tubing to the API gas connection. •...
  • Page 93 Source exhaust connection Holding screw Convoluted liquid waste tubing Blanking fitting Unused source exhaust connection Note: In the figure, the assembly cover is depicted as transparent to reveal the tubing connections. Figure D–7: External source exhaust valve assembly—front configuration Blanking fitting API gas connection Unused source exhaust connection Holding screw...

  • Page 94: Disconnecting The External Source Exhaust Valve Assembly

    Note: In the figure, the assembly cover is depicted as transparent to reveal the tubing connections. D.5.2 Disconnecting the external source exhaust valve assembly Notice: To prevent damage to the instrument, do not use the valve assembly to lift the instrument.
  • Page 95 Required materials • Chemical-resistant, powder-free gloves To connect the source exhaust valve assembly: Requirement: Wear clean, chemical-resistant, powder-free gloves when performing this procedure. To connect the tubing in a rear configuration, ensure that the 90-degree elbow connector on the source exhaust valve is rotated to point toward the rear of the instrument, as shown in the figure below.
  • Page 96 Exhaust hose brackets PTFE exhaust tubing Exhaust trap bottle Source exhaust tube elbow connector Waste liquid drain port Figure D–9: Internal source exhaust valve—front configuration Source exhaust tube elbow connector Waste liquid drain port PTFE source exhaust tube and elbow connector (ensure a downward gradient) Exhaust trap bottle June 15, 2017, 715003956 Rev.

  • Page 97: Disconnecting The Internal Source Exhaust Valve

    D.6.2 Disconnecting the internal source exhaust valve When moving the QDa detector, you must disconnect the source exhaust valve tubing. Perform the following procedure to disconnect the tubing. Note: On Standard QDa detectors, ensure that you disconnect the 6-mm O.D. tube from the diaphragm pump’s 6-mm elbow to the 2.5-L bottle-stopper assembly (shown in the figure "Waste connections"...
  • Page 98 To connect the exhaust-trap bottle: Place the exhaust-trap bottle in the protective bottle stand, and then position the assembly in a readily accessible location below the ACQUITY QDa. Cut the 12-mm PTFE tubing into three lengths (see the figure "External source exhaust valve assembly"...
  • Page 99 Figure D–10: Rear configuration without LC stack or bottom of stack To laboratory exhaust system ACQUITY QDa 90-degree elbow PTFE tubing Exhaust-trap bottle Exhaust solenoid cable PTFE tubing (ensure downward gradient) Liquid waste container Convoluted liquid waste tubing Source exhaust valve assembly...
  • Page 100 "Internal source exhaust valve—front configuration" in Connecting the internal source exhaust valve. Figure D–11: Rear configuration with LC stack ACQUITY QDa 90-degree elbow To laboratory exhaust system PTFE tubing Exhaust-trap bottle Exhaust solenoid cable PTFE tubing (ensure downward gradient) June 15, 2017, 715003956 Rev.

  • Page 101: Alternate Configurations

    Liquid waste container Convoluted liquid waste tubing Source exhaust valve assembly Tip: This figure shows a QDa detector fitted with the external, source exhaust valve assembly (serial number suffix "KAB"). To see the QDa detector fitted with the internal, source exhaust valve (serial number suffix "KAD"), see the figures "Internal source exhaust valve—rear configuration"...
  • Page 102 ACQUITY QDa Source exhaust valve assembly To laboratory exhaust system PTFE tubing Exhaust-trap bottle Exhaust solenoid cable PTFE tubing (ensure downward gradient) Liquid waste container Convoluted liquid waste tubing 90-degree elbow Front exhaust connection Tip: This figure shows a QDa detector fitted with the external, source exhaust valve assembly (serial number suffix "KAB").
  • Page 103 Figure D–13: Front configuration with LC stack ACQUITY QDa Source exhaust valve assembly To laboratory exhaust system PTFE tubing Exhaust-trap bottle Exhaust solenoid cable Liquid waste container Convoluted liquid waste tubing PTFE tubing (ensure downward gradient) June 15, 2017, 715003956 Rev. F...

  • Page 104: Connecting The 2.5-L Waste Container

    90-degree elbow Front exhaust connection Tip: This figure shows a QDa detector fitted with the external source exhaust valve assembly (serial number suffix "KAB"). To see the QDa detector fitted with the internal source exhaust valve (serial number suffix "KAD"), see the figures "Internal source exhaust valve—rear configuration"...
  • Page 105 Figure D–14: Waste connections ACQUITY QDa detector 6-mm elbow Diaphragm pump (Standard QDa only) 4-mm bulkhead fitting (KAB only) 4-mm O.D. drain tubing (KAB only) Route both tubes to the bottle-stopper assembly on the 2.5-L waste container 6-mm O.D. tubing (Standard QDa only) Note: •...
  • Page 106 Notice: To prevent damage to the instrument, ensure that these criteria are met: • The drain tubing is not submerged in solvent within the waste container. • The waste container is not used to collect solvent waste from other sources. Secure the bottle-stopper onto the 2.5-L bottle (see the figure “2.5-L waste container tubing”...

  • Page 107: Connecting The Nitrogen Exhaust Line

    Required materials • Chemical-resistant, powder-free gloves • Utility knife • 12-mm PTFE tubing (included in the Waters Rough Pump Connect Kit) • 12-mm right-angle elbow connectors (included in the Waters Start-Up Kit) Warning: To prevent the nitrogen exhaust from carrying biologically hazardous, toxic, or corrosive LC solvents, you must use a nitrogen exhaust trap bottle and a laboratory exhaust system.

  • Page 108: Connecting The Exhaust Solenoid Cable

    Cut a length of 12-mm tubing long enough to connect the exhaust trap bottle to the laboratory exhaust system. Insert one end of the tubing into the remaining port on the exhaust trap bottle, and route the other end to the laboratory exhaust system. Note: To avoid damaging the instrument, power it off when connecting the solenoid cable.

  • Page 109: Connecting The Workstation

    Figure D–17: Exhaust solenoid connection From instrument exhaust connection (12-mm OD) To laboratory exhaust port (12-mm OD) Exhaust solenoid cable Connect the other end of the cable to the exhaust solenoid connection on the rear panel of the instrument (see External wiring and vacuum connections).

  • Page 110: Connecting To The Workstation

    FCC limits. To make Ethernet connections: Connect one end of one shielded Ethernet cable to the ACQUITY instrument’s network switch, and then connect the free end to the Ethernet card on the preconfigured ACQUITY workstation. June 15, 2017, 715003956 Rev. F...

  • Page 111: Input/Output Signal Connectors

    Connect one end of the other shielded Ethernet cable to the Ethernet port in the top, right- hand corner of the mass spectrometer’s rear panel, and then connect the free end to the ACQUITY instrument’s network switch. D.11 Input/output signal connectors...

  • Page 112: Signal Connections

    Switch (Out) Inject Start (In) Inject Start (In) Event (In) Event (In) Analog (Out) Analog (Out) D.11.1 Signal connections Table D–1: Instrument analog-out/event-in connections Signal connections Description Analog (Out) Used for analog chart output functionality. The output voltage range is 0 to 1 V.
  • Page 113 Figure D–19: Signal connections Connector Signal cable Place the second connection cover over the first cover and snap it into place. Figure D–20: Connection cover Signal connector Connection cover June 15, 2017, 715003956 Rev. F Page 113...

  • Page 114: Connecting To The Power Supply

    D.12 Connecting to the power supply To connect the instrument to the power supply, refer to Connecting to the electricity source. June 15, 2017, 715003956 Rev. F Page 114...

  • Page 115: E Optional Acquity Diverter Valve

    • Do not place the ACQUITY Diverter Valve directly on top of the ACQUITY QDa Detector. You must attach the valve to the instrument using the supplied mounting bracket. • Do not remove the mount fitted to the ACQUITY Diverter Valve. It is used for attaching the valve to the mounting bracket on the QDa.
  • Page 116 Notice: To avoid damaging the QDa or other instruments and devices, you must first de-stack the system. Take the steps necessary to disable each instrument and device in the system before de-stacking it. If the tubing guide is fitted to your instrument, remove it to allow the diverter valve to fit to your instrument.
  • Page 117 Figure E–2: Fitting the ACQUITY diverter valve mounting bracket Two 1/4-inch hex nuts Retaining stud holes Diverter valve mounting bracket Bracket drip tray Retaining studs Screw the hex nuts to the studs using the 1/4-inch hex nut socket wrench. Fit the diverter valve to the mounting bracket by aligning the two hooks on the diverter valve mount on the left side of the valve with the corresponding slots on the mounting bracket, and lower the device until the hooks are securely in the place.
  • Page 118 Figure E–3: Fitting the diverter valve onto the mounting bracket Mounting bracket hook slots Mount hooks E.2.1.1 Removing the tubing guide If a tubing guide is fitted to your instrument, you must remove the guide before fitting the diverter valve assembly to the QDa detector. The tubing guide obstructs the placement of the diverter valve.
  • Page 119 Figure E–4: Removing the QDa front panel QDa interface door QDa switch cover Air filter Tubing guide Front panel Six 4-mm hex-cap retaining bolts Use the #4 POZIDRIV screwdriver to loosen the two #4 POZIDRIV screws fixing the tubing guide to the instrument case, remove the guide, and tighten the screws. June 15, 2017, 715003956 Rev.

  • Page 120: Diverter Valve Wiring

    Fit the source enclosure to the instrument (see Fitting the source enclosure to the instrument). E.2.2 Diverter valve wiring When making connections to the ACQUITY diverter valve's rear panel, refer to the figure "Rear panel connections" in this topic. June 15, 2017, 715003956 Rev. F Page 120...
  • Page 121 9-pin D-Type socket for the contact closure cable USB socket (not used) Power cable socket Diverter valve mount For details of supported system configurations, contact Waters Technical Service. E.2.2.1 Connecting the diverter valve to the QDa Required materials • Contact closure cable •...

  • Page 122: Configuring The Diverter Valve Fluidics

    Connect the male end of the power cord to a suitable wall outlet. E.2.3 Configuring the diverter valve fluidics The ACQUITY diverter valve can be configured to switch the sample flow direction between the QDa and waste in divert mode, or configured in loop injection mode.
  • Page 123 Recommendation: To ensure that the sample flow direction is correct for each command, plumb the connections as shown in this document. Figure E–7: Common configuration of the diverter valve operating in divert mode Valve position 1 - ON = Flow to QDa Valve position 2 - OFF = Flow to waste To waste (blue PEEK tubing, 0.01-inch internal diameter) From LC (red PEEK tubing, 0.005-inch internal diameter)
  • Page 124 Operating the ACQUITY diverter valve E.2.3.1 Diverter valve tubing considerations Where an optical detector such as a PDA or TUV is configured in-line with the ACQUITY QDa Detector and an ACQUITY Diverter Valve, it is important to consider the system flow rate, due to the maximum recommended pressure limit of the flow cell.

  • Page 125: Configuring The Diverter Valve Waste Tubing

    In-line optical detector Maximum flow rate Tubing ACQUITY PDA/TUV 1.25 mL/min Red PEEK tubing, up to 500 mm, 0.005-inch I.D. ACQUITY PDA/TUV 1.0 mL/min Black PEEK tubing, up to 500 mm, 0.004-inch I.D. Alliance 2998/2489 1.5 mL/min Blue PEEK tubing, up to 500 mm, 0.010-inch I.D.

  • Page 126: Operating The Acquity Diverter Valve

    Cut the Tygon tubing and the blue PEEK tubing to the required lengths and feed these into a suitable waste collection vessel, using Y-pieces supplied with the ACQUITY Diverter Valve Assembly Kit for teeing in the tubing as required.

  • Page 127: Manually Operating The Diverter Valve

    Notes: • The events can be configured according to your requirements. • The example is taken from the ACQUITY QDa Detector ICS, version 1.68. E.3.2 Manually operating the diverter valve Manually operate the ACQUITY diverter valve by pressing the front-panel buttons.

  • Page 128: Injecting Sample And Manually Actuating The Diverter Valve

    Figure E–11: Diverter valve manual actuation Digital display: Shows the valve position number (1 or 2) Up arrow: Valve moves to, or stays in, position 2. Down arrow: Valve moves to, or stays in, position 1. E.3.3 Injecting sample and manually actuating the diverter valve To inject sample and manually actuate the diverter valve: Ensure that the QDa is on and mobile phase is flowing from the configured pump.

  • Page 129: Diverter Valve Maintenance Procedures

    Required material • Chemical-resistant, powder-free gloves • Suitable vessel in which to immerse rotor seal and stator when cleaning • 9/64-inch hex wrench supplied with the ACQUITY Diverter Valve Assembly Kit • HPLC-grade (or better) 1:1 methanol/water • Ultrasonic bath •...
  • Page 130 To clean the diverter valve: Stop the flow from the LC, ensure that the QDa detector is in standby mode, and disconnect the power cable from the ACQUITY Diverter Valve. Disconnect all tubing from the valve. Remove the 3 hex bolts securing the valve to the rotor using the 9/64-inch hex wrench.

  • Page 131: Replacing The Diverter Valve Components

    Required materials • Protective eyewear • Chemical-resistant, powder-free gloves • 9/16-inch hex wrench, supplied with the ACQUITY Diverter Valve Assembly Kit • Rotor seal • Stator (Stainless steel or Titanium) To replace the ACQUITY diverter valve stator and rotor seal: Stop the flow from the LC, ensure that the QDa detector is in standby mode, and disconnect the power cable from the diverter valve.
  • Page 132 Stop the flow from the LC, ensure that the QDa detector is in standby mode, and disconnect the power cable from the ACQUITY diverter valve. Disconnect the fluidics tubing from the pod stator on the front of the diverter valve.
  • Page 133 Figure E–13: Removing the diverter valve pod Pod collar Diverter valve pod Valve pod spindle Dispose of the used pod in accordance with local environmental regulations. Slide the new pod into the cuff on the valve, and carefully slot the pod key into the corresponding alignment keyway of the pod cuff on the valve.

  • Page 134: Removing The Diverter Valve Assembly

    Inspect the tubing and fittings prior to reconnecting, and replace these if signs of damage or leaking are visible. E.5 Removing the diverter valve assembly Required materials • 1/4-inch hex nut socket wrench supplied with the ACQUITY Diverter Valve Assembly Kit June 15, 2017, 715003956 Rev. F Page 134...
  • Page 135 Stop the flow from the LC, ensure that the QDa detector is in standby mode, and disconnect the ACQUITY diverter valve power cable from the power supply. De-stack the instruments and devices in the system to obtain sufficient access to the QDa instrument case.
  • Page 136 • To waste (blue PEEK tubing connector, in position 1 or 5, depending on the mode of operation: divert mode/loop injection mode). • Loop between positions 1 and 4 on the diverter valve (loop injection configuration only). Lift the diverter valve upward to free the hooks on the diverter valve mount on the left side of the device casing from the slots on the mounting bracket on the instrument case.

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