Waters ACQUITY UPLC Operator's, Overview And Maintenance Manual page 77

Spectra that produce a large spectral contrast angle
Spectral contrast angle: 62.3°
Compound A
Compound B
Wavelength (nm)
Spectra with similar shapes
In the following figure, the absorbance spectra of two compounds, A and B, are
similar. They therefore produce a small spectral contrast angle (3.0°).
Spectral contrast theory 65