Waters 2489 UV/Visible Detector Manuals

Manuals and User Guides for Waters 2489 UV/Visible Detector. We have 4 Waters 2489 UV/Visible Detector manuals available for free PDF download: Operator's Manual, Overview And Maintenance Manual, Quick Install Manual

Waters 2489 Operator's Manual (234 pages)

UV/Visible Detector

Brand: Waters | Category: Security Sensors | Size: 3 MB

Table of Contents
9
Theory and Principles of Operation
17
1 Theory and Principles of Operation

18
- Detector Description
  18
- Detector Description
  
  19
- Principles of Operation
  
  20
  - Detector Optics
    20
    
    Optics Assembly Light Path
    21
    
    Waters Taperslit Flow Cell
    22
    
    Filtering Noise
    23
  - Wavelength Verification and Test
    25
- Operational Modes
  
  27
  - Single Wavelength Mode
    27
    
    Primary Parameters
    27
    
    Secondary Parameters
    28
  - Dual Wavelength Mode
    28
    
    Chart out Selection Modes
    29
  - Spectrum Scanning
    29
  - Cuvette Operations
    30
  - Ratioplot
    31
  - Maxplot
    31
  - Thermal Wander Management
    31
2 Installing the Detector

33
- Preparing for Installation
  
  34
- Site Selection and Power Requirements
  
  36
  - Site Selection
    36
  - Power Requirements
    37
  - Unpacking and Inspecting
    37
    
    Unpacking
    38
    
    Inspecting
    38
- Making Fluid Line Connections
  
  38
  - Connecting Columns
    39
  - Assembling the Fittings
    40
  - Making Connections
    41
- Making Electrical Power Connections
  
  41
- Connecting to Other Devices
  
  52
3 Preparing the Detector

69
- Initializing the Detector
  
  70
  - Diagnostic Test Failure
    71
- Using the Operator Interface
  
  71
  - Using the Display
    71
    
    Absorbance and Message Icons
    72
  - Using the Keypad
    74
  - Navigating the User Interface
    80
    
    Navigating to and from the Absorbance Screen
    80
    
    Setting up a Run
    81
  - Primary and Secondary Functions
    82
    
    Operating the Trace and Scale Functions
    88
  - Operating Other Detector Functions
    90
    
    Configuring the Detector
    90
    
    Configuring Event Inputs (Contact Closures)
    91
    
    Setting Pulse Periods
    92
    
    Setting Display Contrast
    93
    
    Displaying System Info
    93
    
    Using Help
    94
  - Operating the Detector
    94
    
    Overview of Detector Operation
    94
    
    Operating Modes
    94
    
    Stand-Alone Operation
    95
    
    Remote Control
    95
  - Verifying the Detector
    95
    
    Before You Begin
    95
    
    Recording Sample and Reference Beam Energies
    96
    
    Verifying Peak Response
    97
  - Wavelength Calibration
    98
  - Operating the Detector in Single Wavelength Mode
    99
  - Operating the Detector in Dual Wavelength Mode
    100
    
    Changing from Single to Dual Wavelength Mode
    101
    
    Obtaining a Ratioplot
    102
    
    Obtaining a Maxplot
    103
  - Programming Timed Events, Threshold Events, and Methods
    103
    
    Timed Events
    104
    
    Threshold Events
    107
    
    Storing a Method
    108
    
    Retrieving a Method
    109
    
    Viewing Events Within a Method
    110
    
    Resetting a Method
    110
    
    Clearing Events
    111
- Scanning Spectra
  
  112
  - Before You Begin
    112
  - Scanning New Spectra
    116
    
    Zero Scan
    117
    
    Running the Sample Scan
    120
  - Storing a Spectrum
    124
  - Getting Information about a Stored Spectrum
    124
  - Reviewing a Stored Spectrum
    125
  - Subtracting a Spectrum
    125
  - Replaying a Spectrum
    126
  - Scanning Using the Cuvette
    127
    
    Before You Begin
    128
    
    Cuvette Scanning Procedure
    128
  - Scanning Using a Flow Cell and a Syringe
    130
  - Conserving Lamp Life
    130
  - Shutting down the Detector
    133
    
    Removing Buffered Mobile Phase
    133
4 Maintaining the Detector

135
- Contacting Waters Technical Service
  
  136
- Maintenance Considerations
  
  136
  - Safety Precautions
    136
  - Spare Parts
    136
- Proper Operating Procedures
  
  137
  - Removing the Front Left Panel Cover
    137
  - Routine Maintenance
    138
- Maintaining the Flow Cell
  
  138
  - Flushing the Flow Cell
    139
  - Removing and Cleaning the Flow Cell
    140
  - Disassembling and Reassembling the Flow Cell
    140
    
    Before You Begin
    140
    
    Tools Required
    141
    
    Removing the Flow Cell Assembly
    141
    
    Disassembling the Flow Cell
    144
    
    Inspecting, Cleaning, and Replacing Damaged Flow Cell Components
    147
    
    Rebuilding the Flow Cell
    147
    
    Replacing the Flow Cell
    149
- Replacing the Lamp
  
  150
  - Lamp Characteristics
    150
  - Lamp Energy and Performance
    150
  - When to Replace the Lamp
    151
  - Removing the Lamp
    152
  - Installing the New Lamp
    155
  - Recording the New Lamp Serial Number
    157
  - Setting the Lamp Threshold
    159
- Replacing Fuses
  
  160
5 Error Messages, Diagnostic Tests, and Troubleshooting

164
- Error Messages
  
  164
  - Startup Error Messages
    164
  - Error Messages Preventing Operation
    167
- User-Selected Diagnostic Tests
  
  170
- Troubleshooting
  
  181
  - When You Contact Waters
    181
  - Diagnostic Tests
    182
  - Power Surges
    182
  - Hardware Troubleshooting
    182
A Safety Advisories

186
- Warning Symbols
  
  186
  - Task-Specific Hazard Warnings
    186
  - Warnings that Apply to Particular Instruments, Instrument Components, and
    187
    
    Burst Warning
    187
    
    Mass Spectrometer Flammable Solvents Warning
    187
    
    Mass Spectrometer Shock Hazard
    188
    
    Biohazard Warning
    188
    
    Chemical Hazard Warning
    189
- Caution Symbol
  
  189
- Warnings that Apply to All Waters Instruments
  
  189
- Electrical and Handling Symbols
  
  197
  - Electrical Symbols
    197
  - Handling Symbols
    198
B Detector Specifications

200
- Operational Specifications
  
  200
- Optical Specifications
  
  202
- Optional Waters Taperslit Flow Cell Specifications
  
  203
C Spare Parts

205
D Solvent Considerations

208
- Introduction
  
  208
  - Clean Solvents
    208
  - Solvent Quality
    208
  - Preparation Checklist
    208
  - Water
    208
  - Using Buffers
    208
  - Tetrahydrofuran
    209
- Solvent Miscibility
  
  209
  - How to Use Miscibility Numbers
    211
- Buffered Solvents
  
  212
- Head Height
  
  212
- Solvent Viscosity
  
  212
- Mobile Phase Solvent Degassing
  
  212
  - Gas Solubility
    213
    
    Effects of Intermolecular Forces
    213
    
    Effects of Temperature
    213
    
    Effects of Partial Pressure
    213
- Solvent Degassing Methods
  
  214
  - Sparging
    214
  - Vacuum Degassing
    214
  - Solvent Degassing Considerations
    214
    
    Vacuum Degassing
    215
- Wavelength Selection
  
  215

Waters 2489 Operator's Manual (194 pages)

UV/Visible Detector

Brand: Waters | Category: Security Sensors | Size: 2 MB

General Information
3
Trademarks
3
Customer Comments
3
Safety Considerations
4
Safety Advisories
5
Canada Spectrum Management Emissions Notice
5
Electrical Power Safety Notice
5
Safety Hazard Symbol Notice
5
Equipment Misuse Notice
6
Operate this Instrument
6
Applicable Symbols
7
Audience and Purpose
7
Intended Use of the 2489 Uv/Visible Detector
7
Calibrate
8
Quality-Control
8
ISM Classification
8
ISM Classification: ISM Group 1 Class B
8
EC Authorized Representative
9
Table of Contents
11
Theory and Principles of Operation
17
Detector Description
17
Principles of Operation
19
Detector Optics
19
Wavelength Verification and Test
24
Operational Modes
25
Single-Wavelength Mode
25
Dual-Wavelength Mode
27
Spectrum Scanning
28
Cuvette Operations
29
Ratioplot
29
Maxplot
30
Thermal-Wander Management
30
Install the Detector
31
Prepare to Install the Detector
32
Site Selection and Power Requirements
33
Site Selection
33
Power Requirements
35
Unpack and Inspect
35
Make Plumbing Connections
36
Make Electrical Power Connections
38
Make Signal Connections from Rear Panel and Ethernet Connectors to the Detector
40
Make Ethernet Connections
43
Connect to Other Devices
49
Connect the Detector to Empower Using an E-SAT/IN Module
50
Connect the Detector to a 745/745B/746 Data Module
53
Connect the Detector to a Fraction Collector
55
Prepare the Detector
57
Initialize the Detector
57
Idle Mode
59
Use the Operator Interface
59
Use the Display
59
Using the Keypad
63
Navigate the User Interface
68
Primary and Secondary Functions
70
Operate Other Detector Functions
78
Operate the Detector
82
Verify that the Detector Operates Properly
83
Wavelength Calibration
86
Operate the Detector in Single-Wavelength Mode
86
Operate the Detector in Dual-Wavelength Mode
87
Program Timed Events, Threshold Events, and Methods
90
Scan Spectra
97
Before You Begin
98
Scan New Spectra
100
Store a Spectrum
107
Review a Stored Spectrum
109
Subtract a Spectrum
109
Replay a Spectrum
110
Scan Using the Cuvette
110
Scan Using a Flow Cell and a Syringe
112
Conserve Lamp Life
113
Shutting down the Detector
115
Maintaining the Detector
117
Contact Waters Technical Service
117
Maintenance Considerations
118
Safety and Handling
118
Spare Parts
119
Proper Operating Procedures
119
Routine Maintenance
119
Maintain the Flow Cell
120
Flush the Flow Cell
120
Remove and Clean the Flow Cell
122
Disassemble and Reassemble the Flow Cell
122
Replace the Lamp
129
Removing the Lamp
131
Install the New Lamp
134
Record the New Lamp Serial Number
136
Set the Lamp Threshold
138
Replace Fuses
138
Error Messages, Diagnostic Tests, and Troubleshooting
141
Error Messages
141
Startup Error Messages
141
Error Messages Preventing Operation
144
User-Selected Diagnostic Tests
148
Overview
148
Use the Diagnostic Tests
150
Service Diagnostic Tests
157
Troubleshoot
157
When You Contact Waters
158
Diagnostic Tests
158
Power Surges
158
Hardware Troubleshooting
158
A Safety Advisories
161
Warning Symbols
162
Task-Specific Hazard Warnings
162
Specific Warnings
163
Caution Advisory
166
Warnings that Apply to All Waters Instruments
167
Warnings that Address the Replacing of Fuses
172
Electrical Symbols
173
Handling Symbols
174
B Specifications
175
Operational Specifications
176
Optional Waters Taperslit Flow Cell Specifications
178
C Solvent Considerations
181
Introduction
181
Preventing Contamination
181
Clean Solvents
181
Solvent Quality
182
Preparation Checklist
182
Water
182
Using Buffers
182
Tetrahydrofuran
182
Solvent Miscibility
183
How to Use Miscibility Numbers
185
Buffered Solvents
186
Head Height
186
Solvent Viscosity
186
Mobile Phase Solvent Degassing
186
Gas Solubility
187
Solvent Degassing Methods
187
Sparging
188
Vacuum Degassing
188
Solvent Degassing Considerations
188
Wavelength Selection
189
UV Cutoffs for Common Solvents
189
Mixed Mobile Phases
190
Wavelength Selection for Chromophore Detection
191

Waters 2489 Overview And Maintenance Manual (150 pages)

UV/Visible Detector

Brand: Waters | Category: Security Sensors | Size: 2 MB

Trademarks
3
Customer Comments
3
Contacting Waters
4
Safety Considerations
4
Safety Hazard Symbol Notice
4
Considerations Specific to the 2489 Uv/Visible Detector
4
FCC Radiation Emissions Notice
5
Electrical Power Safety Notice
5
Equipment Misuse Notice
5
Applicable Symbols
5
Intended Use of the 2489 Uv/Visible Detector
6
Calibrating
6
Quality Control
6
EMC Considerations
7
Canada Spectrum Management Emissions Notice
7
ISM Classification: ISM Group 1 Class B
7
EC Authorized Representative
7
Table of Contents
9
Theory and Principles of Operation
15
Detector Description
15
Principles of Operation
17
Detector Optics
17
Wavelength Verification and Test
21
Operational Modes
22
Single-Wavelength Mode
22
Dual-Wavelength Mode
24
Spectrum Scanning
25
Cuvette Operations
26
Ratioplot
26
Maxplot
26
Thermal-Wander Management
26
Installing the Detector
27
Before You Begin
27
Unpacking and Inspecting
27
Unpacking the Detector
27
Inspecting the Detector
28
Selecting a Laboratory Site
28
Stacking System Modules
29
Connecting to the Electricity Source
30
Connecting Signal Cables
31
Connecting I/O Cables
32
Connecting Signal Cables from the Rear Panel and Ethernet Connectors to the Detector
33
Making Ethernet Connections
33
Starting a Method
35
Turning the Detector Lamp on or off
36
Connecting the Detector to the 2695 Separations Module
36
Connecting the Detector to a Chromatography Data Workstation Using an E-SAT/IN Module
38
Connecting the Detector to a 745/745B/746 Data Module
41
Connecting the Detector to a Fraction Collector
42
Plumbing the Detector
43
Connecting Columns in HPLC Systems
44
Connecting Columns in ACQUITY Arc Systems
44
Assembling the Fittings (HPLC Only)
45
Making Tubing Connections in HPLC Systems
45
Making Tubing Connections in ACQUITY Arc Systems
45
Prepare the Detector
47
Initializing the Detector
47
Diagnostic Test Failure
48
Idle Mode
48
Using the Operator Interface
49
Using the Display
49
Using the Keypad
51
Navigating the User Interface
55
Primary and Secondary Functions
57
Operating Other Detector Functions
63
Operating the Detector
66
Verifying that the Detector Operates Properly
67
Wavelength Calibration
69
Operating the Detector in Single-Wavelength Mode
69
Operating the Detector in Dual-Wavelength Mode
70
Scanning Spectra
78
Before You Begin
78
Scanning New Spectra
80
Storing a Spectrum
86
Reviewing a Stored Spectrum
87
Subtracting a Spectrum
87
Replaying a Spectrum
88
Scanning Using the Cuvette
88
Scanning Using a Flow Cell and a Syringe
90
Conserving Lamp Life
90
Shutting down the Detector
92
Maintaining the Detector
93
Contacting Waters Technical Service
93
Maintenance Considerations
93
Safety and Handling
94
Spare Parts
94
Proper Operating Procedures
94
Routine Maintenance
94
Maintaining the Flow Cell
95
Flushing the Flow Cell
95
Removing and Cleaning the Flow Cell
97
Disassembling and Reassembling the Flow Cell
98
Replacing the Lamp
103
Lamp Characteristics
104
Lamp Energy and Performance
104
Removing the Lamp
105
Installing the New Lamp
107
Recording the New Lamp Serial Number
109
Setting the Lamp Threshold
110
Replacing Fuses
111
Error Messages, Diagnostic Tests, and Troubleshooting
113
Error Messages
113
Startup Error Messages
113
User-Selected Diagnostic Tests
113
Overview
113
Using the Diagnostic Tests
115
Service Diagnostic Tests
120
Troubleshooting
121
Diagnostic Tests
121
Power Surges
121
Hardware Troubleshooting
121
Lamp Troubleshooting
123
A Safety Advisories
125
Warning Symbols
125
Specific Warnings
126
Notices
127
Bottles Prohibited Symbol
128
Required Protection
128
Warnings that Apply to All Waters Instruments and Devices
128
Warnings that Address the Replacing of Fuses
131
Electrical and Handling Symbols
133
Electrical Symbols
133
Handling Symbols
134
B Specifications
137
Physical Specifications
137
Environmental Specifications
137
Electrical Specifications
138
Performance Specifications
139
Optical Component Specifications
139
Flow Cell Specifications
140
C Solvent Considerations
141
Introduction
141
Preventing Contamination
141
Clean Solvents
141
Solvent Quality
141
Preparation Checklist
141
Water
142
Using Buffers
142
Tetrahydrofuran
142
Solvent Miscibility
142
How to Use Miscibility Numbers
143
Buffered Solvents
144
Head Height
144
Solvent Viscosity
144
Mobile Phase Solvent Degassing
145
Gas Solubility
145
Solvent Degassing Methods
146
Sparging
146
Vacuum Degassing
146
Solvent Degassing Considerations
146
Wavelength Selection
147
UV Cutoffs for Common Solvents
147
Mixed Mobile Phases
148
Wavelength Selection for Chromophore Detection
149

Waters 2489 Quick Install Manual (4 pages)

UV/Visible Detector

Brand: Waters | Category: Security Sensors | Size: 0 MB

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Waters 2489 UV/Visible Detector Manuals

Waters 2489 Operator's Manual (234 pages)

Table of Contents

1 Theory and Principles of Operation

Detector Description

Principles of Operation

Operational Modes

2 Installing the Detector

Preparing for Installation

Site Selection and Power Requirements

Making Fluid Line Connections

Making Electrical Power Connections

Connecting to Other Devices

3 Preparing the Detector

Initializing the Detector

Using the Operator Interface

Scanning Spectra

4 Maintaining the Detector

Contacting Waters Technical Service

Maintenance Considerations

Proper Operating Procedures

Maintaining the Flow Cell

Replacing the Lamp

Replacing Fuses

5 Error Messages, Diagnostic Tests, and Troubleshooting

Error Messages

User-Selected Diagnostic Tests

Troubleshooting

A Safety Advisories

Warning Symbols

Caution Symbol

Warnings that Apply to All Waters Instruments

Electrical and Handling Symbols

B Detector Specifications

Operational Specifications

Optical Specifications

Optional Waters Taperslit Flow Cell Specifications

C Spare Parts

D Solvent Considerations

Introduction

Solvent Miscibility

Buffered Solvents

Head Height

Solvent Viscosity

Mobile Phase Solvent Degassing

Solvent Degassing Methods

Wavelength Selection

Waters 2489 Operator's Manual (194 pages)

Table of Contents

Waters 2489 Overview And Maintenance Manual (150 pages)

Table of Contents

Waters 2489 Quick Install Manual (4 pages)

Table of Contents

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