Related Manuals for Thermo Scientific Dionex IonPac AS32-Fast-4mm Analytical
Summary of Contents for Thermo Scientific Dionex IonPac AS32-Fast-4mm Analytical
- Page 1 Dionex IonPac AS32-Fast-4µm Columns 065763 Revision 01 • March 2019 For Research Use Only. Not for use in diagnostic procedures.
- Page 2 Dionex IonPac AG32-Fast-4µm Guard Columns × 30 mm (Item # 303152) × 30 mm (Item # 303154) Thermo Scientific Product Manual for Dionex IonPac AS32-Fast-4µm Columns Page 2 of 42 065763-01 For Research Use Only. Not for use in diagnostic procedures.
- Page 3 Terms and Conditions of Sale, which Terms and Conditions of Sale shall govern all conflicting information between the two documents. Revision History: Revision 01, March 2019; Original Publication. Thermo Scientific Product Manual for Dionex IonPac AS32-Fast-4µm Columns Page 3 of 42 065763-01...
- Page 4 IMPORTANT test results; or might contain information that is critical for optimal performance of the system. Highlights helpful information that can make a task easier. Thermo Scientific Product Manual for Dionex IonPac AS32-Fast-4µm Columns Page 4 of 42 065763-01 For Research Use Only. Not for use in diagnostic procedures.
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Page 5: Table Of Contents
Introduction..................7 Dionex IonPac AS32-Fast-4µm/Dionex IonPac AG32-Fast-4µm Column Packing Specifications ........................7 Installation ..................9 Thermo Scientific Dionex High Pressure Ion Chromatography Systems ......... 9 System Requirements ........................... 10 System Voi d Volume............................. 10 Column Start-Up ............................10 Dionex IonPac Guard Columns ......................... 10 Sample Concentrators.......................... - Page 6 Column Cleanup Procedure .......................... 39 Appendix B – Quality Assurance Reports ..........40 Appendix C – Additional Information ..........42 General Information on PEEK™ Tubing ....................42 Thermo Scientific Product Manual for Dionex IonPac AS32-Fast-4µm Columns Page 6 of 42 065763-01...
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Page 7: Introduction
– Introduction 1. Introduction The Thermo Scientific™ Dionex™ IonPac™ AS32-Fast-4µm column is designed for analysis of low polarity anions, polysulfonated aromatics, aromatic dyes, and pigments. Its selectivity allows for sub-ppb quantification of perchlorate in high chloride and sulfate matrices using suppressed conductivity and mass spectrometry detection. - Page 8 Exceeding the maximum flow rates listed in the above table can disrupt the uniformity of the packing of the column bed and irreversibly damage the performance of the column. WARNING Thermo Scientific Product Manual for Dionex IonPac AS32-Fast-4µm Columns Page 8 of 42 065763-01 For Research Use Only.
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Page 9: Installation
– Installation Installation Read the instrument manuals. This manual assumes that you are using Thermo Scientific Dionex instrumentation and are familiar with the installation and operation of the Thermo Scientific Dionex Ion Chromatograph (IC). If you do not understand the operation of the... -
Page 10: System Requirements
Dionex IonPac columns are designed to be run on Dionex Ion Chromatographs equipped with suppressed conductivity detection. We recommend the use of ferrules and fittings rated with a pressure of >5000 psi. The use of Thermo Scientific Dionex IC PEEK Viper fittings is recommended to achieve consistent low dead volume connections and ensure optimum chromatographic performance. -
Page 11: Sample Concentrators
Injecting larger numbers of moles of a sample can result in overloading the column which can affect the detection linearity. For low concentrations of analytes, larger injection loops can be used to increase sensitivity. Thermo Scientific Product Manual for Dionex IonPac AS32-Fast-4µm Columns Page 11 of 42 065763-01 For Research Use Only. -
Page 12: Dionex Anion Dynamically Regenerated Suppressor Requirements
Suppressor, see Document No. 031956, the “Product Manual for the Dionex DRS 600.” 2.9 Dionex Anion Chemically Regenerated Suppressor Requirements A Thermo Scientific Dionex Anion Chemically Regenerated Suppressor (Dionex ACRS 500) may be used instead of a Dionex ADRS 600 for applications that require suppressed conductivity detection. -
Page 13: Operation
For assistance, visit Unity Lab Services online at www.unitylabservices.com. From the U.S., call the Customer Care Center for Dionex Products at 1 -800-346-6390. Outside the U.S., call the nearest Thermo Fisher Scientific office. NOTE Thermo Scientific Product Manual for Dionex IonPac AS32-Fast-4µm Columns Page 13 of 42 065763-01... -
Page 14: General Operational Precautions
Therefore, only use solvent in the eluent when needed for improved resolution NOTE of analytes of interest. Thermo Scientific Product Manual for Dionex IonPac AS32-Fast-4µm Columns Page 14 of 42 065763-01... -
Page 15: Eluent Preparation
Eluent Generation It is recommended that Dionex IonPac columns are used with Dionex HPIC Systems equipped with a Thermo Scientific Dionex Eluent Generator Cartridge. The use of eluent generation provides a more stable baseline, improved performance, and increased reproducibility compared to the use of manually prepared eluents. -
Page 16: Manually Prepared Eluents
(nitrogen can be used if eluents do not contain solvents). Contamination from carbon dioxide when basic eluents are exposed to the air can cause performance variability such as retention time shifts. Thermo Scientific Product Manual for Dionex IonPac AS32-Fast-4µm Columns Page 16 of 42 065763-01 For Research Use Only. -
Page 17: Example Applications
Care”. If your sample matrices are relatively low in ionic concentration, you may be able to increase the sensitivity of your system by using sample concentration techniques. Thermo Scientific Product Manual for Dionex IonPac AS32-Fast-4µm Columns Page 17 of 42 065763-01 For Research Use Only. -
Page 18: Isocratic Elution With And Without A Guard Column
Dionex IonPac AS32-Fast-4µm 2 x 150 mm 1. Fluoride 1.25 2. Bromide 3. Iodide 10.0 4. Perchlorate 5. Sulfate Minutes Thermo Scientific Product Manual for Dionex IonPac AS32-Fast-4µm Columns Page 18 of 42 065763-01 For Research Use Only. Not for use in diagnostic procedures. -
Page 19: Analysis Of Polythionates
16 mA Trithionate Peaks: mg/L 1. Dithionate 25.0 2. Trithionate 25.0 3. Tetrathionate 25.0 Dithionate Minutes Thermo Scientific Product Manual for Dionex IonPac AS32-Fast-4µm Columns Page 19 of 42 065763-01 For Research Use Only. Not for use in diagnostic procedures. -
Page 20: Analysis Of Aromatic Sulfonates, Pigments, And Dyes
9. FDC Red #40 Dionex AERS 500, 4mm AutoSuppression, recycle mode Suppressor Current: 198 mA µS Minutes Thermo Scientific Product Manual for Dionex IonPac AS32-Fast-4µm Columns Page 20 of 42 065763-01 For Research Use Only. Not for use in diagnostic procedures. - Page 21 9. FDC Red #40 Dionex AERS 500, 2mm AutoSuppression, recycle mode Suppressor Current 50 mA µS Minutes Thermo Scientific Product Manual for Dionex IonPac AS32-Fast-4µm Columns Page 21 of 42 065763-01 For Research Use Only. Not for use in diagnostic procedures.
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Page 22: Analysis Of Perchlorate In High Chloride And Sulfate Matrices
75 mA Peaks: mg/L 1. Chloride 1000 2. Perchlorate 3. Carbonate 1000 4. Sulfate 1000 Minutes Thermo Scientific Product Manual for Dionex IonPac AS32-Fast-4µm Columns Page 22 of 42 065763-01 For Research Use Only. Not for use in diagnostic procedures. - Page 23 µS Peaks: mg/L 1. Chloride 1000 2. Perchlorate 3. Carbonate 1000 4. Sulfate 1000 -0.1 Minutes Thermo Scientific Product Manual for Dionex IonPac AS32-Fast-4µm Columns Page 23 of 42 065763-01 For Research Use Only. Not for use in diagnostic procedures.
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Page 24: Analysis Of Persulfate
Dionex AERS 500, 2mm AutoSuppression, recycle mode µS Suppressor Current 19 mA Peaks: mg/L 1. Sulfate 2. Persulfate Minutes Thermo Scientific Product Manual for Dionex IonPac AS32-Fast-4µm Columns Page 24 of 42 065763-01 For Research Use Only. Not for use in diagnostic procedures. -
Page 25: Troubleshooting
Clean Column 5.2.4 Insufficient Equilibration Extend Equilibration T ime at 5.4.3A the Start of the Gradient Run Thermo Scientific Product Manual for Dionex IonPac AS32-Fast-4µm Columns Page 25 of 42 065763-01 For Research Use Only. Not for use in diagnostic procedures. - Page 26 Purge contamination, employ a 5.4.2 Efficiency Loss sample or system trap or guard column, clean or replace column Thermo Scientific Product Manual for Dionex IonPac AS32-Fast-4µm Columns Page 26 of 42 065763-01 For Research Use Only. Not for use in diagnostic procedures.
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Page 27: High Back Pressure
If this is the case, additional tightening may not seal the column but instead CAUTION damage the column tube or the end fitting. Thermo Scientific Product Manual for Dionex IonPac AS32-Fast-4µm Columns Page 27 of 42 065763-01... -
Page 28: Filter Eluent
B. Make sure that the eluents are made from chemicals with the recommended purity. C. Make sure that the deionized water used to prepare the reagents has a specific resistance of 18.2 megohm-cm. Thermo Scientific Product Manual for Dionex IonPac AS32-Fast-4µm Columns Page 28 of 42 065763-01 For Research Use Only. -
Page 29: A Contaminated Trap Column
Inconsistent Retention Times Inconsistent or shifting retention times could be due to one of several different factors. These should be checked to determine the cause and address the issue. Thermo Scientific Product Manual for Dionex IonPac AS32-Fast-4µm Columns Page 29 of 42 065763-01 For Research Use Only. -
Page 30: Drifting To Shorter Retention Time
When an injection valve is actuated, the timing is critical for consistent retention times. This will occur when the injection valve needs to be cleaned or retorqued. Refer to the valve manual for troubleshooting and service procedures. Thermo Scientific Product Manual for Dionex IonPac AS32-Fast-4µm Columns Page 30 of 42 065763-01 For Research Use Only. -
Page 31: Poor Peak Resolution
2 mm systems. Cut the tubing lengths as short as possible, checking to ensure a smooth, cut. The use of Thermo Scientific Dionex IC PEEK Viper fittings is recommended to achieve consistent low dead volume connections and ensure optimum chromatographic performance. -
Page 32: Analyte Specific Efficiency Loss
B. Check sample as source of contamination. C. Employ the use of an appropriate guard or trap column to remove contaminants. Contact your local representative for current product specific information. Thermo Scientific Product Manual for Dionex IonPac AS32-Fast-4µm Columns Page 32 of 42 065763-01 For Research Use Only. -
Page 33: Shortened Retention Times
Be especially careful to make sure that the recommended chemicals are used. The deionized water should have a specific NOTE resistance of 18.2 megohm-cm. Thermo Scientific Product Manual for Dionex IonPac AS32-Fast-4µm Columns Page 33 of 42 065763-01... -
Page 34: Loss Of Front-End Resolution
Small baseline disturbances at the beginning or at the end of the chromatogram can be overlooked so long as they do not interfere with the quantification of the peaks of interest. Thermo Scientific Product Manual for Dionex IonPac AS32-Fast-4µm Columns Page 34 of 42 065763-01 For Research Use Only. -
Page 35: Appendix A - Column Care
Currently at Thermo Fisher Scientific, we have obtained consistent results using Optima® Solvents by Fisher Scientific. We have found that bottled HPLC-grade water from Burdick & Jackson contains acceptably low levels of impurities. Thermo Scientific Product Manual for Dionex IonPac AS32-Fast-4µm Columns Page 35 of 42 065763-01 For Research Use Only. -
Page 36: Inorganic Chemicals
Solvents and water should be premixed in concentrations which allow proper mixing by the gradient pump to minimize outgassing. Ensure that all of the inorganic chemicals are soluble in the highest solvent concentration to be used during the analysis. Thermo Scientific Product Manual for Dionex IonPac AS32-Fast-4µm Columns Page 36 of 42 065763-01 For Research Use Only. -
Page 37: Column Cleanup
< 5% levels and the ionic strength of the eluent to < 50 mM levels to avoid creating high pressure zones in the column that may disrupt the uniformity of the column packing. Thermo Scientific Product Manual for Dionex IonPac AS32-Fast-4µm Columns... -
Page 38: Choosing The Appropriate Cleanup Solution
Organic solvents can be used alone if the contamination is nonionic and hydrophobic. The degree of nonpolar character of the solvent should be increased as the degree of hydrophobicity of the contamination within the range of acceptable solvents. Thermo Scientific Product Manual for Dionex IonPac AS32-Fast-4µm Columns Page 38 of 42 065763-01 For Research Use Only. -
Page 39: Column Cleanup Procedure
Reconnect the Dionex IonPac Analytical Column to the Dionex Suppressor and if your system was originally configured with a guard column, place the guard column in lin e between the injection valve and the analytical column. Thermo Scientific Product Manual for Dionex IonPac AS32-Fast-4µm Columns Page 39 of 42 065763-01 For Research Use Only. -
Page 40: Appendix B - Quality Assurance Reports
– Appendix B – Quality Assurance Reports 7. Appendix B – Quality Assurance Reports Thermo Scientific Product Manual for Dionex IonPac AS32-Fast-4µm Columns Page 40 of 42 065763-01 For Research Use Only. Not for use in diagnostic procedures. - Page 41 – Appendix B – Quality Assurance Reports Thermo Scientific Product Manual for Dionex IonPac AS32-Fast-4µm Columns Page 41 of 42 065763-01 For Research Use Only. Not for use in diagnostic procedures.
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Page 42: Appendix C - Additional Information
441.0 58.0 Note: Blue and Light blue are very similar in color. Additionally, tubing from different suppliers may differ to that stated above. Thermo Scientific Product Manual for Dionex IonPac AS32-Fast-4µm Columns Page 42 of 42 065763-01 For Research Use Only. Not for use in diagnostic procedures.
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