Fluid Transfer - Labcyte Echo 520 User Manual

3.3.7 Fluid transfer

Volumetric Accuracy: <10% error (single source plate-wide average from
target volume)
Volumetric Precision: <8% CV
DMSO/water concentration: <8% accuracy error; <5% precision CV
(Echo 550 and 555 models only)
Fluid temperature: 21°C ± 5°C (70°F± 9°F)
Compound should be in liquid form prior to transfer.
Throughput: Transfer time for fluids in different plates and on different
Echo systems.
1
2
3
4
5
6
7
8
9
10
1
2
3
4
5
6
7
8
9
10
1
These plate type names are used with instruments that have shipped with Echo Server 2.4. Instruments that
shipped with earlier versions of software may use a different naming terminology and may have different
transfer specifications. Contact your Labcyte account representative with any questions for your specific
instrument.
1
Plate calibration 384PP_AQ_CP works with a much wider range of fluids. There are use
cases (particularly in protein crystallography applications) where the volumetric accuracy
may deviate from this specification. Please contact your Labcyte account representative
with any questions.
3-20 Echo Liquid Handler User Manual
1
Table 3.2 Fluid throughput comparison
1
Plate type
384PP_DMSO2
384PP_AQ_BP2
384PP_AQ_CP
384PP_AQ_GP2
384PP_AQ_SP2
384LDV_DMSO
384LDV_AQ_B
1536LDV_DMSO
1536LDV_DMSO_HT
1536HB_DMSO
384PP_DMSO2
384PP_AQ_BP2
384PP_AQ_CP
384PP_AQ_GP2
384PP_AQ_SP2
384LDV_DMSO
384LDV_AQ_B
1536LDV_DMSO
1536LDV_DMSO_HT
1536HB_DMSO
Transfer Echo 555
volume (nL) (min)
2.5 1.4
2.5 1.8
2.5 2.7
2.5 1.8
2.5 1.7
2.5 1.5
2.5 1.5
2.5 2.6
2.5 2.6
2.5 3.1
100 1.9
100 3.1
100 6.0
100 3.1
100 2.2
100 2.0
100 2.8
100 5.8
100 4.7
100 6.2
Echo 550 Echo 520
(min) (min)
2.5 2.5
2.9 --
3.7 --
2.9 --
2.7 --
2.6 2.6
2.6 --
4.5 4.4
-- --
5.0 5.0
3.8 7.7
4.2 --
7.2 --
4.2 --
3.3 --
3.9 7.7
3.9 --
8.2 12.7
-- --
10.2 25.5