Labcyte Echo 520 User Manual page 136

Focus calibration indicator
Periodically, the focal length of the acoustic transducer should be checked.
When this period of time has expired, the F C indicator
OCUS ALIBRATION
turns yellow. The user should perform a focus calibration as soon as
possible. See "Focus calibration check" on page 5-50.
Green. Focus calibration has been performed recently.
Yellow. A Focus calibration should be performed as soon as possible.
Coupling fluid temperature and indicator
The coupling fluid temperature inside the
Echo liquid handler is reported in degrees
Celsius. The correct operating range for the
coupling fluid temperature is 22.1°C ± 0.9°C. The coupling fluid controls
the temperature of the acoustic transducer. A correct and stable acoustic
transducer temperature is required to maintain accurate and precise liquid
transfers.
The coupling fluid temperature indicator reports three states:
Green. Coupling fluid temperature is OK.
Yellow. Coupling fluid temperature warning: The coupling fluid
temperature is outside of the normal range: 21.2°C–23.0°C.
Red. Coupling fluid temperature fault: The coupling fluid temperature is
outside of the warning range: 20.2°C – 24.0°C.
RF Temp 1 temperature
The temperature inside the Echo controller is reported in degrees Celsius.
The temperature sensor monitors one of the most critical internal electronic
devices used to control the RF system. Normal RF T 1 temperature is
EMP
between 50–55°C. As the reported temperature increases to 55°C or
above, it is possible that source plate survey or liquid transfer accuracy will
be affected.
5-36 Echo Liquid Handler User Manual