Labcyte Echo 520 User Manual page 154

Liquid handler
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If the source plate fluid is stable, the survey interval can be safely increased.
For example, "every 5 plates" will cause a new survey to be performed
when the source plate and first destination plate are loaded, and every fifth
destination plate thereafter. Increasing the survey interval may improve
throughput.
Default: 1.
Assume Homogeneous Plate: When a
source plate is surveyed to measure the
DMSO/water ratio in each well, the Echo
liquid handler creates a DMSO concentration map of the entire plate. This
map is post-processed using a moving average filter if the A
H
OMOGENEOUS
with this processing. Typically, better liquid transfers are obtained using
post-processing when the source microplate contents are homogeneous. A
homogeneous source microplate is one where the solution to be
transferred is very similar, well-to-well.
If the U
Plate feature is unavailable, and its checkbox is "grayed out."
Default: Assume homogeneous plate off.
Power Monitoring: Selecting the P
M
ONITORING
to monitor the RF power used to eject each fluid droplet. Labcyte
recommends that power monitoring not be enabled by the user unless
directed to do so by Labcyte personnel. Enabling power monitoring adds
significant overhead to each droplet ejection and decreases liquid transfer
throughput.
Default: Power monitoring off.
Save Plate-Survey Data to Disk: The data
collected during a source plate survey is held in
cache memory. It is replaced when the next
source plate survey is performed. If the S
P
LATE
stored on the client PC disk. The data file is stored to <InstallDir>\Labcyte\
Medman\Data. This data can be useful to Labcyte personnel during
diagnostic troubleshooting.
Default: Source plate survey data is not saved on the Echo controller.
5-54 Echo Liquid Handler User Manual
Note: The survey interval can be set to 0; however, with this setting
the protocol will not survey the plate.
P
checkbox is selected. There is no overhead associated
LATE
P
checkbox is selected, the Assume Homogeneous
SE
REDEFINED
checkbox causes the Echo liquid handler
-S
D
D
URVEY
ATA TO
OWER
AVE
checkbox is selected, the survey data will be
ISK
SSUME

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