Refill The Coupling Fluid Bottle - Labcyte Echo 520 User Manual

6.2.2 Refill the coupling fluid bottle

The Echo coupling fluid couples the energy from the acoustic transducer to
the bottom of the source plate. Bubbles in the tubing (from very low fluid
level) or contamination in the fluid can reduce the accuracy or precision of
source plate surveys and fluid transfers. The coupling bottle should be
refilled (or cleaned and refilled) immediately. Maintaining clean coupling
fluid at the proper fill level ensures a high level of performance in the Echo
liquid handler.
Refill the coupling fluid bottle when one of the following occurs:
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Materials required
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1. Ensure that no fluid transfer or diagnostic procedures are under way.
2. Turn off the vacuum and coupling fluid pump.
a. Turn off the coupling fluid pump through the D
b. Unplug the vacuum line to decrease vacuum pressure.
3. Unscrew the cap on the coupling fluid bottle and remove the bottle
from its caddy. Be careful not to lift the coupler fluid tube (straight tube)
above the water level or you will introduce air into the line. If you have
air in the line, you will need to purge, then recycle the fluid through the
system for at least 15 minutes.
page 6-11
6-8 Echo Liquid Handler User Manual
C F indicator on the Echo software D
OUPLING LUID
yellow, indicating that the coupling fluid has dropped to the refill
required level. See "Coupling fluid indicator" on page
LCD panel W
ARNING
coupling fluid level.
Echo ActiveX events report that the coupling fluid has dropped to the
refill required level. Refer to the Labcyte Echo Integration Manual.
Distilled water, sufficient to fill the 1L bottle to ~900 mL
Caution: Do not use de-ionized water, as it may corrode the fluidics
system
50 μL (one drop) algaecide (Labcyte catalog number ECHO-AL04)
Gloves for protection when handling the algaecide
Paper towels or shop cloths to deal with any unexpected fluid leaks
the Echo software.
Note: The surge tank will add a few seconds to stopping the vacuum.
and "Cycle fluid through the Echo system" on page
indicator has turned yellow as a result of the low
See "Fault light" on page
See "Purge the fluid circuit" on
tab is
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5-35.
3-10.
window of
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6-13.