Labcyte Echo 520 User Manual page 103

Source Destinatio
plate n plate
384-well 96-well
384-well
384-well
384-well 1536-well
384-well 3456-well
1536-well 96-well
1536-well 384-well
1536-well
1536-well
1 Destacking: transferring liquid from a single source (src) plate to several destination (dest) plates
2 Stacking: transferring liquid from several source plates to a single destination plate
Examples of protocols that cannot be created with the standard Echo
software included with the Echo liquid handler installation:



* Compound from the source plate wells are transferred to the same relative well posi-
tions in the destination plate. For example, transferring compound from the first two
columns in the source plate to the first two columns in the destination plate.
Table 5.1 Liquid transfer types
1
destacking quadrant
(4 regions from 1 src plate to 4 dest plates)
standard (1 src plate to 1 dest plate)
2
stacking quadrant
(4 src plates to 4 regions on 1 dest plate)
stacking quadrant
(9 src plates to 9 regions on 1 dest plate)
destacking quadrant
(16 regions from 1 src plate to 16 dest
plates)
destacking quadrant
(4 regions from 1 src plate to 4 dest plates)
standard (1 src plate to 1 dest plate)
Note: See "What types of liquid transfer are supported?" on
page 1-12 for definitions of plate mapping terms.
Transfers other than replication
Variable drop volume transfers. For example, 5 nL from source well
A1 to destination well A1, 10 nL from source well A2 to destination
well A2, and so on.
Transfers to non-standard destination plates.
Note: Complex and non-standard liquid transfer protocols can be
created by using the Echo Cherry Pick and Echo Plate Reformat
applications, or by programming your own custom protocols with the
Echo ActiveX programming interface (refer to the Labcyte Echo
Integration Manual).
Transfer map type
(full or partial)
destacking interleaved
(2x2, or every other dest well)
stacking interleaved
(2x2, or every other dest well)
stacking interleaved
(3x3, or every third dest well)
destacking interleaved
(4x4, or every fourth dest
well)
destacking interleaved
(2x2, or every other dest well)
*
. For example, well A1 into well C12.
Echo Liquid Handler Software 5-3