Labcyte Echo 520 User Manual page 173

Cycle fluid through the Echo system
Caution: The chiller does not need to be running for this procedure,
however, do not cycle coupling fluid through the Echo liquid handler
that is below 22°C or above ambient room temperature. Extreme
temperatures can damage the acoustic transducer.
1. Turn on the coupling fluid pump. The coupling fluid will be pumped into
the Echo liquid handler. After three to five minutes, verify that the
coupling fluid is returning to the coupling fluid bottle and that it does
not contain a large number of bubbles.
2. To completely purge the Echo fluidic circuit, repeat the empty-purge-fill
procedure two more times.
3. After filling the fluidic circuit, the coupling fluid bottle will be partially
empty. Turn the coupling fluid pump off, and add coupling fluid so that
the bottle contains 875 mL of coupling fluid. Make sure that the J-
shaped tube isn't covered by the new fluid level.
4. If repeated empty/fill cycles have been performed, remember to add
algaecide at the ratio of 50 μL per 1000 mL of distilled water.
5. Turn on the coupling fluid pump.
6. Check the C
OUPLING
window. The indicator should be green.
Note: If the empty-purge-fill procedure is being performed more than
once, add the algaecide only during the last fill of the coupling fluid
bottle.
Empty and clean the waste water bottle
The Echo liquid handler dries each source plate before it is extended
outside of the process door. To dry the source plate, the Echo liquid
handler vacuums coupling fluid from the bottom of the source plate and
sends the fluid to the waste water bottle.
Note: Newer Echo systems do not use a waste water bottle.
.
Empty the waste water bottle when any of the following occur:
 The W B
ASTE OTTLE
software is red, indicating that the waste water has reached the
empty waste bottle required level.
 The F indicator on the front panel of the Echo liquid handler is
AULT
red as a result of the full waste water bottle.
 Three months have passed since the bottle was emptied.
F status on the Echo software D
LUID
indicator on the D
IAGNOSTICS
tab of the Echo
IAGNOSTICS
Maintenance and Service 6-13