Labcyte Echo 520 User Manual page 169

4. Visually inspect the used coupling fluid. It should not be cloudy or
visually contaminated with algae or other substances. If it is, clean the
bottle (see "Clean and refill the coupling fluid bottle" on page
before refilling it.
5. Fill the coupling fluid bottle with 900 mL of distilled water and add
1 drop (50 μL) of algaecide.
Note: The ratio of algaecide to water is optimized for deterring the
growth of algae while minimizing residual moisture on the bottom of
the plate. One drop per 900 mL is sufficient.
6. Replace the bottle cap. Hand-tighten the cap securely to ensure a tight
seal.
7. Carefully inspect the coupler fluid tube and attached flexible tubing for
the presence of bubbles. If you see bubbles, you will need to purge, then
recycle the fluid through the system after you complete this procedure.
See "Purge the fluid circuit" on page 6-11
Echo system" on page
8. Reconnect the vacuum line and turn the coupling fluid pump on through
the D window.
IAGNOSTIC
Note: The surge tank will add a few seconds to starting the vacuum.
Caution: Make sure that the vacuum line is connected and working
correctly before turning the coupling fluid pump back on.
If the vacuum line is disconnected, or the vacuum system is not operating
correctly, or the cap on the fluid bottle is not tightly sealed when the
coupling fluid pump is turned on, the coupling fluid can flood the bottom
of the system.
6-13.
and "Cycle fluid through the
Maintenance and Service 6-9
6-10)