Clean And Refill The Coupling Fluid Bottle - Labcyte Echo 520 User Manual

6.2.3 Clean and refill the coupling fluid bottle

Clean and refill the fluid coupling bottle when one of the following
occurs:
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The following procedures are needed to clean and refill the coupling fluid
bottle.
1. Empty the coupling fluid bottle
2. Purge the fluid circuit
3. Clean and refill the coupling fluid bottle
4. Cycle fluid through the Echo system
This section describes each task in detail.
Tools and materials needed
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The fluidic system will be emptied, purged, and refilled several times,
therefore take note of the following caution:
6-10 Echo Liquid Handler User Manual
Fluid appears cloudy.
Two weeks have passed since the last coupling fluid refill.
Fluid transfer accuracy or precision has been affected by coupling
fluid contamination.
Note: During periods of heavy duty cycle use, refilling the coupling
fluid bottle will be necessary more frequently than once every two
weeks. It is acceptable to skip the complete fluidic circuit purge as long
as it is performed at least every second week.
4 liters of distilled water. The Echo fluidic system holds about
1100 mL of fluid (approximately 225 mL in the fluidic circuit and
875 mL in the coupling fluid bottle). Emptying, purging, and filling
the system will be performed several times.
Caution: Do not use de-ionized water, as it may corrode the fluidics
system.
An empty, clean 1000 mL coupling fluid bottle (square bottle catalog
number ECHO-AL15-S; round bottle catalog number ECHO-AL-15-R)
50 μL of algaecide (Labcyte catalog number ECHO-AL04)
Gloves for protection when handling the algaecide
Paper towels or shop cloths to deal with any unexpected fluid leaks
Screwdriver for removing the fuse cover
Caution: When working with the Echo coupling fluid system and
turning its coupling fluid pump on and off, make sure that the
vacuum line is connected and working before turning the coupling
fluid pump back on. If the vacuum line is disconnected or the vacuum