Simulations; Using Simulation Features; Esp Pelleting; Simulate Menu - Beckman Coulter Optima L-XP Instructions For Use Manual

Using Simulation Features

When running a separation using CsCl, it is critical to keep the salt from precipitating out and
potentially causing a rotor mishap. This is accomplished by running the rotor below the speed at
which the salt precipitates. ESP (Efficient Sedimentation Program) was developed as a way of
predicting optimum run conditions for pelleting RNA over a wide variety of rotor and tube
combinations. ESP accomplishes this by calculating the movement of three components in solution:
• RNA, ranging in size from 0.1 to 3kb,
• A hypothetical contaminant of chromosomal DNA, and
• CsCl
Movement of each component is monitored as a function of speed and considers the geometry of
different rotor and tube combinations. The program starts out at the maximum rated speed of the
rotor (less if the overall solution density exceeds the limit of the rotor/tube combination selected),
and simulates a sequence of steps reducing the speed of the rotor just prior to precipitation of
the CsCl.
You access the simulations windows from the Simulate menu shown in
the optimized run program generated from these simulated run parameters to the Settings window
and start the run, or save the simulation for later use. You can also print out the simulation (see
APPENDIX A, Preinstallation
Figure 3.1 Simulate Menu

ESP Pelleting

Pelleting separates particles of different sedimentation coefficients, the largest particles in the
sample traveling to the bottom of the tube first. ESP simulates the formation of gradient and
PN LXP-IM-7AB
Requirements, for printer hookup instructions).
CHAPTER 3

Simulations

Figure 3.1. You can transfer
3-1