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PowerPlex ESI 17 Fast System
Promega PowerPlex ESI 17 Fast System Manuals
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Promega PowerPlex ESI 17 Fast System manual available for free PDF download: Technical Manual
Promega PowerPlex ESI 17 Fast System Technical Manual (92 pages)
Applied Biosystems Genetic Analyzers
Brand:
Promega
| Category:
Measuring Instruments
| Size: 7 MB
Table of Contents
Table of Contents
2
Description
4
Product Components and Storage Conditions
5
Before You Begin
6
Precautions
6
Spectral Calibration
7
Protocols for DNA Amplification Using the Powerplex ® ESI 17 Fast System
7
Amplification Of Extracted Dna In A 25Μl Reaction Volume
8
Amplification Of Extracted Dna In A 25Μl Reaction Volume
9
Direct Amplification Of Dna From Storage Card Punches In A 25Μl Reaction Volume
11
Direct Amplification Of Dna From Swabs In A 25Μl Reaction Volume
15
Instrument Setup and Sample Preparation
18
Detection of Amplified Fragments Using the Applied Biosystems ® 3500 or 3500Xl Genetic Analyzer
18
Detection of Amplified Fragments Using the Applied Biosystems ® 3130 or 3130Xl Genetic Analyzer with Data Collection Software, Version 3.0 or 4.0
30
Data Analysis
33
Importing Powerplex ® ESI Fast Panels, Bins and Stutter Text Files into Genemapper ® ID-X Software
33
Importing the WEN ILS 500 ESS Size Standard into Genemapper ® ID-X Software
34
Creating a Size Standard with Genemapper ® ID-X Software
34
Creating a Casework Analysis Method with Genemapper ® ID-X Software
36
Creating a Databasing or Paternity Analysis Method with Genemapper ® ID-X Software
41
Importing Powerplex ® ESI Fast Panels and Bins Text Files into Genemapper ® ID Software, Version 3.2
44
Importing the WEN ILS 500 ESS Size Standard into Genemapper ID Software, Version 3.2
45
Creating a Size Standard with Genemapper ® ID Software, Version 3.2
46
Creating a Casework Analysis Method with Genemapper ® ID Software, Version 3.2
48
Creating a Databasing or Paternity Analysis Method with Genemapper ® ID Software, Version 3.2
52
Controls
55
Results
55
Troubleshooting
59
Amplification And Fragment Detection
59
Amplification Of Extracted Dna
62
Direct Amplification Of Dna From Storage Card Punches
64
Direct Amplification Of Dna From Swabs
68
Genemapper ® ID-X Software
71
Genemapper ® ID Software
73
References
77
Appendix
79
Advantages of Using the Loci in the Powerplex ® ESI 17 Fast System
79
The Wen Internal Lane Standard 500 Ess
83
Direct Amplification Of Dna From Storage Card Punches In A 12.5Μl Reaction Volume
83
Direct Amplification Of Dna From Swabs In A 12.5Μl Reaction Volume
88
Composition of Buffers and Solutions
91
Related Products
92
Summary Of Changes
92
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