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Figure 23. Protocols screen.
Promega Corporation
2800 Woods Hollow Road • Madison, WI 53711-5399 USA
Toll Free in USA 800-356-9526 • Phone 608-274-4330
Maxwell
®
16 MDx Instrument Technical Manual
2.
3. A confirmation screen allows you to verify that the
!
B. Setting Up Samples and Collecting Input Data
Setting Up Samples: No Run Data Reporting
Please refer to your specific Maxwell
Technical Bulletin or Manual for detailed instructions
regarding hardware mode, sample preparation, cartridge
setup, and purification method.
•
Select FFPE/Cells to purify DNA from Formalin-
Fixed Paraffin-Embedded (FFPE) tissue (use with
the Maxwell
®
16 FFPE Tissue LEV DNA
Purification Kit, Promega Cat.# AS1130) or Cells
®
(use with the Maxwell
Purification Kit, Promega Cat.# AS1140).
•
Select RNA to purify RNA from tissue (use with
the Maxwell
®
16 Tissue LEV Total RNA Kit,
Promega Cat.# AS1220), and cells (the Maxwell
Cell LEV Total RNA Purification Kit, Promega Cat.#
AS1225).
•
Select Viral to purify Viral total nucleic acid from
serum or plasma (use with the Maxwell
Total Nucleic Acid Purification Kit, Promega Cat.#
AS1150).
•
Select the User option to access and run custom
protocols. Instructions for setting customized
protocols are presented in Section 10.A. Select the
method required.
•
Select the Other option to access specialized
protocols for Homogenization or Fixed Yield DNA
and to access a Demo option.
Homogenization allows limited homogenization of
some types of samples prior to running the
purification method. See Section 10.B.
Fixed Yield DNA allows purification of a limited
amount of DNA, allowing a reproducible amount
of DNA to be captured between samples.
The Demo protocol is for demonstration purposes
only and should not be used for sample purification.
Please contact Promega Technical Services at
techserv@promega.com for more information on
these specialized options.
correct selection has been made. If correct, press the
Run/Stop button, and you will be prompted to open
the door to load the samples. Press the Run/Stop button.
Open the door. The instrument platform will extend,
ready for sample loading. Follow the steps in Sections
5.B and 5.C to gather the run data and perform the run.
Follow the steps in Section 6 to generate the run reports.
Important. At this point there are different paths
forward, depending on whether the run data reporting
options are being used. See Section 5.B for more
information.
16 Cell LEV DNA
®
16
®
16 Viral
®
16 Purification Kit
part# TM320
rev. 9/09
12
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