Reaction Tray Operation - Siemens ADVIA 1800 Operator's Manual

For analysis, the reaction tray rotates the cuvette in front of the spectrophotometer, which
measures the absorbance of the cuvette. After analysis, the reaction washer (WUD)
washes the cuvettes.
The RRV contains 221 reusable cuvettes (13 sets of 17 cuvettes). Each cuvette contains 80
to 300 µL of reaction liquid.
For sample analysis, the RRV cuvettes are kept at a constant temperature of 37°C by being
immersed in the reaction tank, which contains an oil-heat bath.

Reaction tray operation

At initialization, the tray rotates counterclockwise until RRV cuvette 1 is in the position
where the first reagent is dispensed.
After you start a run, the reaction tray moves RRV cuvette 1 counterclockwise to the
reagent 1 dispense position. Then the following cycle begins:
1. R1 dispenses into the RRV cuvette.
2. The cuvette moves a half-turn to the sample dispense position.
3. At the same time, an empty cuvette moves to the R1 dispense position, beginning the
analysis cycle for the next sample.
4. Sample is dispensed into the cuvette.
5. After three seconds, the cuvette moves another half-turn to the mixer 1 (MIXR1)
position.
The second cuvette moves to the sample dispense position, and an empty cuvette moves to
the R1 dispense position (three positions from MIXR1).
During each half-turn, the spectrophotometer measures the absorbance of the liquid in
each of the cuvettes that pass it (across all 14 wavelengths).
6. MIXR1 mixes the reagent and sample, completing the 6-second measurement cycle
(spectrophotometer readings occur every other half-turn for the reaction time required
by the test).
7. The system continuously dispenses R1 and sample into subsequent cuvettes and are
mixed in the cuvettes, until all tests complete and data is collected.
When required by assay conditions, the RRV stops for the dispensing and mixing of R2.
You can view measurement data at the Reaction Monitor window.
8. After the required measurements complete, the system washes the cuvettes used for
the assay, and checks the lamp energy.
During each half-turn, unused cuvettes are washed with cell conditioner. Then a cell blank
measurement is performed. You can view cell blank results in the Reaction Monitor
window.
If an assay is not running, you can operate the unit manually at the Manual Operation
window.
30 ADVIA 1800 Operator's Guide