Priming And Initializing The Ise Module; Replacing Ise Electrodes; Removing Electrodes - Siemens ADVIA 1800 Operator's Manual

Priming and initializing the ISE module

1. At the ISE Operation window, in the Bufferprime area, enter
2. To prime the line with buffer, select
3. When the priming is finished, verify that the electrodes are not leaking.
4. At the ISE Operation window, select
5. Run 10 pooled serum samples, or do an ISE CV check.
6. Perform calibration and run controls.

Replacing ISE electrodes

Materials required:
Electrodes
• Cl (REF 07097504, PN 073-0049-01)
• K (REF 06135445, PN 073-0050-01)
• Na (REF 03092699, PN 073-0051-01)
• Reference (REF 00311764, PN 073-0653-01
• O-rings, 3 (REF 09955206, PN 073-0071-01)
• Philips screwdriver
Time: 5 minutes
Analyzer mode: Manual operation
Replace the Na, K, and the Cl electrodes if the slope is incorrect or calibration
continuously fails.
The acceptable ISE slope is between 45.0 and
63.0. Slopes outside of this range are flagged
as shown in the table. A flagged slope fails
the calibration. The slope limits are defined at
the ISE Parameter Settings window.
Replace the reference electrode when the reference electrode value is <500.
Checking the reference electrode value
1. At the Menu Panel, select
2. At the ISE Monitor window, at the bottom of the Calib.monitor: Serum area, check
the value of the Ref. electrode field.
3. If the Ref. electrode value is

Removing electrodes

1. At the Menu Panel, select
2. In the Period. wash area, select
3. Using a Phillips screwdriver, remove the screws (
DPP shield to the analyzer panel.
174
, then select
Execute
, then select
Exit
Mark
., then select
Maint ISE Monitor
, replace the reference electrode.
less than 500.0
, then select
Maint. ISE Operation
, then select
OFF Set
1
in the Times field.
15
.
Yes
.
Yes
BIOHAZARD
Wear personal protective equipment.
Use universal precautions.
ISE Slope Range
H > 65.0
h 63.1 to 65.0
l 38.0 - 44.9
L < 38.0
.
.
.
, see Figure 5-2) that secure the
ADVIA 1800 Operator's Guide