Reaction Tank; Spectrophotometer - Siemens ADVIA 1800 Operator's Manual

Reaction tank

The reaction tank contains non-reactive oil, which keeps the temperature of the liquid in
the reaction tray (RRV) cuvettes at a constant 37°C ±0.5°C. A heater and a thermostat
control the temperature.
Figure 1-25. Reaction tank

Spectrophotometer

The spectrophotometer measures the amount of light absorbed at 14 specific wavelengths
by liquids contained in reaction cuvettes.
Every six seconds, the reaction tray (RRV) moves cuvettes containing reaction liquid
(sample and reagent) in front of a halogen lamp, which sends light through the cuvettes.
Each time, a different wavelength is measured.
The photometer then measures the absorbance based on the lamp energy and the optical
density of the cuvettes. This process is repeated for as many times and wavelengths as
required by the assay conditions.
A cooling tank maintains the lamp temperature.
34
1 Reaction Tank
2 Two Liquid Surface Level
Sensors
ADVIA 1800 Operator's Guide