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4
Using the Pump
Preparing the Binary Pump
Preparing the Binary Pump
For best performance of the pump:
• Place solvent cabinet with the solvent bottles always on top (or at a higher
• For optimum performance, use the built-in degasser. This is mandatory for
• When using the pump with vacuum degassing unit, flush the degassing unit
• Prevent blocking of solvent inlet filters (never use the pump without solvent
• Check pump outlet filters and column frit in regular time intervals. A
• Whenever possible use a minimum flow rate of 5 µL/min per solvent
• When using buffer solutions, flush the system with water before switching
• The optional seal wash function should be used when buffer solutions with
• Never leave an unused pump with water in a channel for an extended
42
level) of the pump.
flow rates below 0.5 mL/min and for configurations without Jet Weaver.
with at least 5 mL per channel before operating the pump, especially when
the pumping system had been turned off for a certain length of time (for
example, overnight) and volatile solvent mixtures are used in the channels.
inlet filters). Growth of algae should be avoided, see
Systems"
on page 43.
blocked pump outlet filter can be identified by black, yellow or greenish
layers on its surface.
channel to avoid crossflow of solvent into the unused pump channel.
it off.
concentrations of 0.1 M or higher are being pumped for long periods of
time.
period of time (2-3 days). Always flush with organic solvent or add 10 %
isopropanol to water.
"Algae Growth in HPLC
Agilent 1290 Infinity Binary Pump User Manual
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