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Agilent Technologies 1290 Infinity II User Manual

Agilent Technologies 1290 Infinity II User Manual

Variable wavelength detector
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  • Page 1 Agilent 1290 Infinity II Variable Wavelength Detector User Manual Agilent Technologies...
  • Page 2 1987) or any equivalent agency regulation or contract clause. Use, duplication or dis- closure of Software is subject to Agilent Technologies’ standard commercial license terms, and non-DOD Departments and Agencies of the U.S. Government will Agilent 1290 Infinity II VWD User Manual...
  • Page 3 In This Book In This Book This manual covers the Agilent 1290 Infinity II Variable Wavelength Detector (G7114B). Find information on other Agilent Variable Wavelength Detectors in separate manuals. 1 Introduction to the Variable Wavelength Detector This chapter gives an introduction to the detector, instrument overview and internal connectors.
  • Page 4 This chapter describes the detector in more detail on hardware and electronics. 13 LAN Configuration This chapter provides information on connecting the module to the Agilent ChemStation PC. 14 Appendix This chapter provides addition information on safety, legal and web. Agilent 1290 Infinity II VWD User Manual...

  • Page 5: Table Of Contents

    Set up the Detector with Agilent Open Lab ChemStation The Detector User Interface Detector Control Settings Method Parameter Settings Scanning with the VWD 4 Preparing the Module Leak and Waste Handling Setting up an Analysis Solvent Information Agilent 1290 Infinity II VWD User Manual...
  • Page 6 9 Maintenance Introduction to Maintenance Warnings and Cautions Overview of Maintenance Cleaning the Module Remove and Install the Doors Replace the Deuterium Lamp Replace the Flow Cell / Cuvette Holder Repairing the Flow Cells Agilent 1290 Infinity II VWD User Manual...
  • Page 7 11 Identifying Cables Cable Overview Analog Cables Remote Cables CAN/LAN Cables RS-232 Cables USB Cables 12 Hardware Information Firmware Description Electrical Connections Interfaces Setting the 6-bit Configuration Switch Instrument Layout Early Maintenance Feedback Agilent 1290 Infinity II VWD User Manual...
  • Page 8 Manual Configuration PC and Agilent ChemStation Setup 14 Appendix General Safety Information Waste Electrical and Electronic Equipment Directive Radio Interference Sound Emission Solvent Information Declaration of Conformity for HOX2 Filter Agilent Technologies on Internet Agilent 1290 Infinity II VWD User Manual...

  • Page 9: Introduction To The Variable Wavelength Detector

    Agilent 1290 Infinity II VWD User Manual Introduction to the Variable Wavelength Detector Introduction to the Detector Product Description Optical System Overview Flow Cell Lamp Source Lens Assembly Entrance Slit Assembly Filter Assembly Mirror Assemblies M1 and M2 Grating Assembly...

  • Page 10: Introduction To The Detector

    • built- in electronic temperature control (ETC) for improved baseline stability, and • built- in holmium oxide filter for fast wavelength accuracy verification. Agilent 1290 Infinity II VWD User Manual...

  • Page 11: Product Description

    Introduction to the Variable Wavelength Detector Product Description Product Description The Agilent 1290 Infinity II Variable Wavelength Detector (VWD) is the most sensitive and fastest detector in its class. Time- programmable wavelength switching provides sensitivity and selectivity for your applications.

  • Page 12: Optical System Overview

    This configuration allows fast change of the wavelength. The cutoff filter is moved into the lightpath above 370 nm to reduce higher order light. Figure 2 Optical Path of the Variable Wavelength Detector Agilent 1290 Infinity II VWD User Manual...

  • Page 13: Optical System Overview

    Outlet i.d. 0.30 0.17 0.17 0.17 Outlet length Total volume 60.77 14.49 14.00 60.77 µL Materials in contact SST, quartz, PTFE, SST, quartz, PTFE SST, quartz, PTFE SST, quartz, Kapton with solvent PEEK Agilent 1290 Infinity II VWD User Manual...
  • Page 14 Entrance Slit Assembly The entrance slit assembly has an exchangeable slit. The standard one has a 1- mm slit. For replacement and calibration purposes to optimize the alignment, a slit with a hole is needed. Agilent 1290 Infinity II VWD User Manual...
  • Page 15 > 370 nm HOLMIU holmium oxide filter for wavelength check SHUTTER for measurement of dark current of photo diodes A photo sensor determines the correct position. Agilent 1290 Infinity II VWD User Manual...
  • Page 16 The photo diode current is directly converted to digital data direct photo current digitalization. The data is transferred to the detector main board . The photo diode ADC boards are located close to the photo diodes. Agilent 1290 Infinity II VWD User Manual...

  • Page 17: Dual-Wavelength Mode

    • Wavelength settings are timetable programmable (depends if enough time for implementation is available), • switching from Single- Wavelength mode to Dual- Wavelength mode is NOT timetable programmable, • filter settings are not timetable programmable. Agilent 1290 Infinity II VWD User Manual...

  • Page 18: Leak And Waste Handling

    Introduction to the Variable Wavelength Detector Leak and Waste Handling Leak and Waste Handling The 1290 Infinity II Series has been designed for safe leak and waste handling. It is important that all security concepts are understood and instructions are carefully followed.

  • Page 19: Leak And Waste Handling

    • from the Seal Wash Sensor • from the pump's Purge Valve or Multipurpose Valve The waste tube connected to the leak pan outlet on each of the bottom instruments guides the solvent to a suitable waste container. Agilent 1290 Infinity II VWD User Manual...

  • Page 20: Waste Concept

    Agilent recommends using the 6 L waste can with 1 Stay Safe cap GL45 with 4 ports (5043-1221) for optimal and safe waste disposal. If you decide to use your own waste solution, make sure that the tubes don't immerse in the liquid. Agilent 1290 Infinity II VWD User Manual...

  • Page 21: Operating Principle

    Introduction to the Variable Wavelength Detector Operating Principle Operating Principle Figure 6 Hydraulic path Agilent 1290 Infinity II VWD User Manual...

  • Page 22: Operating Principle

    Introduction to the Variable Wavelength Detector Operating Principle Agilent 1290 Infinity II VWD User Manual...

  • Page 23: Site Requirements And Specifications

    Agilent 1290 Infinity II VWD User Manual Site Requirements and Specifications Site Requirements Physical Specifications Performance Specifications G7114B This chapter gives information on environmental requirements, physical and performance specifications. Agilent Technologies...

  • Page 24: Site Requirements

    ➔ Make sure the power connector of the instrument can be easily reached and unplugged. ➔ Provide sufficient space behind the power socket of the instrument to unplug the cable. Agilent 1290 Infinity II VWD User Manual...
  • Page 25 Never operate your instrumentation from a power outlet that has no ground connection. ➔ Never use a power cord other than the Agilent Technologies power cord designed for your region. Use of unsupplied cables WA R N I N G Using cables not supplied by Agilent Technologies can lead to damage of the electronic components or personal injury.
  • Page 26 ➔ If your module was shipped in cold weather, leave it in its box and allow it to warm slowly to room temperature to avoid condensation. Agilent 1290 Infinity II VWD User Manual...

  • Page 27: Physical Specifications

    Up to 2000 m (6562 ft) Non-operating altitude Up to 4600 m (15092 ft) For storing the module Safety standards: Installation category II, Pollution degree 2 For indoor use only. IEC, EN, CSA, UL Agilent 1290 Infinity II VWD User Manual...

  • Page 28: Performance Specifications G7114B

    Site Requirements and Specifications Performance Specifications G7114B Performance Specifications G7114B Performance Specifications Table 3 Agilent 1290 Infinity II Variable Wavelength Detector (G7114B) Performance Specifications Feature Specification Detection type Double-beam photometer Light source Deuterium lamp Number of signals Single and dual wavelength detection...
  • Page 29 Site Requirements and Specifications Performance Specifications G7114B Table 3 Agilent 1290 Infinity II Variable Wavelength Detector (G7114B) Performance Specifications Feature Specification Flow cells Standard: 14 µL volume, 10 mm cell path length and 40 bar (588 psi) pressure maximum Micro: 2 µL volume, 3 mm cell path length and 120 bar (1760 psi) pressure maximum Semi-micro: 5 µL volume, 6 mm cell path length and 40 bar (588 psi)
  • Page 30 Performance tests should be done with a completely warmed up optical unit (> one hour). ASTM measurements require that the detector should be turned on at least 24 hours before start of testing. Agilent 1290 Infinity II VWD User Manual...

  • Page 31: Using The Module

    Agilent 1290 Infinity II VWD User Manual Using the Module Magnets Turn on/off Status Indicators Instrument Configuration Set up the Detector with Agilent Open Lab ChemStation The Detector User Interface Detector Control Settings Method Parameter Settings Scanning with the VWD This chapter explains the essential operational parameters of the module.

  • Page 32: Magnets

    Using the Module Magnets Magnets Agilent 1290 Infinity II VWD User Manual...

  • Page 33: Turn On/Off

    Using the Module Turn on/off Turn on/off Power switch (1) On (2) Off Agilent 1290 Infinity II VWD User Manual...

  • Page 34: Status Indicators

    (for example a leak or defective internal components). 5. Resident mode (blinking) - for example during update of main firmware. 6. Bootloader mode (fast blinking). Try to re-boot the module or try a cold-start. Then try a firmware update. Agilent 1290 Infinity II VWD User Manual...

  • Page 35: Instrument Configuration

    All switches DOWN: module uses the default IP address 192.168.254.11. b Switch 4 UP and others DOWN: module uses DHCP. 2 Enter the setup information (MAC / IP address and/or Instrument Name). a Agilent OpenLab ChemStation (Configure Instrument): Agilent 1290 Infinity II VWD User Manual...
  • Page 36 Using the Module Instrument Configuration b Lab Advisor (Instrument Overview - Add Instrument): Agilent 1290 Infinity II VWD User Manual...

  • Page 37: Set Up The Detector With Agilent Open Lab Chemstation

    Figure 7 ChemStation Method and Run Control (just detector is shown) After successful load of the OpenLab ChemStation, you should see the module as an active item in the graphical user interface (GUI). Agilent 1290 Infinity II VWD User Manual...

  • Page 38: The Detector User Interface

    The information provides flow cell and lamp related information like • Part number • Production date • Serial number and other details. Agilent 1290 Infinity II VWD User Manual...
  • Page 39 • Identify Module (Status LED will blink) • Perform a Balance • Switch the UV-lamp on/off (same as click on button “Make Device Ready/Turn device off (standby)”) • Take / Abort Scans (during flow off) Agilent 1290 Infinity II VWD User Manual...

  • Page 40: Detector Control Settings

    RFID tags. If no RFID tag cell is used, detector icon will become gray (cell tag not ready) and analysis is disabled. • At Power On: automatic lamp-on at power on. • Automatic Turn On: automatic detector power on. Agilent 1290 Infinity II VWD User Manual...

  • Page 41: Method Parameter Settings

    Figure 8 Method parameter settings For additional help and support: Highlight the desired cell and press F1. A help screen will N O T E open with additional information and documentation about the topic. Agilent 1290 Infinity II VWD User Manual...
  • Page 42 (As Pump/Injector) or the module (if different from system stop time). The data collection is stopped at this time. A posttime period can be used to allow module’s items to equilibrate (e.g. after gradient change or temperature change). Agilent 1290 Infinity II VWD User Manual...
  • Page 43 Scan Range / Step: Stop-Flow scan range / step. Access to the scan feature is only possible during run. See “Scanning with the VWD” on page 45. Additional Signals (for diagnostic purpose) • Sample only • Reference only Agilent 1290 Infinity II VWD User Manual...
  • Page 44 These signals can be used in addition to the normal baseline signal to determine whether correlation to temperature or voltage/current of the lamp. These signals are available via the Agilent ChemStation Online Plot/Data Signal and/or Agilent Lab Advisor Software. Agilent 1290 Infinity II VWD User Manual...

  • Page 45: Scanning With The Vwd

    Turning off the pump would stop the run and no access to the sample scan is possible. N O T E Agilent 1290 Infinity II VWD User Manual...
  • Page 46 Online Spectra Window 6 Select Sample Scan. A sample scan is taken in the range defined under Miscellaneous in Table 4 on page 42 and displays the result (Sample Scan minus Blank Scan). Agilent 1290 Infinity II VWD User Manual...

  • Page 47: Preparing The Module

    Agilent 1290 Infinity II VWD User Manual Preparing the Module Leak and Waste Handling Waste Concept Setting up an Analysis Before Using the System Requirements and Conditions Preparing the Detector Preparing the HPLC System Running the Sample and Verifying the Results...

  • Page 48: Leak And Waste Handling

    Recommendations for Solvent Cabinet N O T E For details, see the usage guideline for the Agilent 1200 Infinity Series Solvent Cabinets. For correct installation of your system contact your Agilent service representative. Agilent 1290 Infinity II VWD User Manual...

  • Page 49: Waste Concept

    Agilent recommends using the 6 L waste can with 1 Stay Safe cap GL45 with 4 ports (5043-1221) for optimal and safe waste disposal. If you decide to use your own waste solution, make sure that the tubes don't immerse in the liquid. Agilent 1290 Infinity II VWD User Manual...

  • Page 50: Setting Up An Analysis

    (when available in the system) and the pump. Solvents containing volatile ingredients will slightly lose these. Therefore priming of the pumping system is required before starting an application. Agilent 1290 Infinity II VWD User Manual...
  • Page 51 2 Flush all tubes with at least 30 mL of solvent. 3 Set flow to required value of your application and close the purge valve. Pump for approximately 10minutes before starting your application. N O T E Agilent 1290 Infinity II VWD User Manual...

  • Page 52: Requirements And Conditions

    52: Table 8 Conditions Flow 1.5 mL/min Stoptime 8 min Solvent 100% (30% water/70% Acetonitrile) Temperature Ambient Wavelength sample 254 nm Injection Volume 1 µL Column Temperature (optional): 25 °C or ambient Agilent 1290 Infinity II VWD User Manual...
  • Page 53 53. The exact profile of the chromatogram will depend on the chromatographic conditions. Variations in solvent quality, column packing, standard concentration and column temperature will all have a potential effect on peak retention and response. Figure 12 Typical Chromatogram with UV-detector Agilent 1290 Infinity II VWD User Manual...

  • Page 54: Preparing The Detector

    Sampler (prepare the standard isocratic sample into a vial). e Solvents (fill water and Acetontrile into the solvent bottles). 5 Load the default method. 6 Pump the water/acetonitrile (30/70 %) mobile phase through the column for 10 min for equilibration. Agilent 1290 Infinity II VWD User Manual...
  • Page 55 Preparing the Module Setting up an Analysis 7 Select the menu item Run Control > Sample Info and enter information about this application. Click OK to leave this screen. Figure 13 Sample Info Agilent 1290 Infinity II VWD User Manual...

  • Page 56: Running The Sample And Verifying The Results

    ChemStation will show the resulting chromatogram. Figure 14 Chromatogram with Isocratic Test Sample Information about using the Data Analysis functions can be obtained from the Using your N O T E ChemStation manual supplied with your system. Agilent 1290 Infinity II VWD User Manual...

  • Page 57: Solvent Information

    (typically 20 – 25 °C, 68 – 77 °F). If corrosion is possible, it usually accelerates at higher temperatures. If in doubt, please consult technical literature on chemical compatibility of materials. Agilent 1290 Infinity II VWD User Manual...
  • Page 58 It has limited stability with aliphatic, aromatic and halogenated hydrocarbons, THF, phenol and derivatives, concentrated acids and bases. For normal phase applications, the maximum pressure should be limited to 200 bar. Agilent 1290 Infinity II VWD User Manual...
  • Page 59 For example, a 1 % solution of acetic acid in methanol will attack steel. • Solutions containing strong complexing agents (for example, EDTA, ethylene diamine tetra- acetic acid). • Mixtures of carbon tetrachloride with 2- propanol or THF. Agilent 1290 Infinity II VWD User Manual...
  • Page 60 TFE/PDD copolymer tubings, which are used in all Agilent degassers except 1322A, are not compatible with fluorinated solvents like Freon, Fluorinert, or Vertrel. They have limited life time in the presence of Agilent 1290 Infinity II VWD User Manual...
  • Page 61 Sapphire, Ruby and Al -based ceramics Sapphire, ruby and ceramics based on aluminum oxide Al are inert to almost all common acids, bases and solvents. There are no documented incompatibilities for HPLC applications. Agilent 1290 Infinity II VWD User Manual...
  • Page 62 Preparing the Module Solvent Information Agilent 1290 Infinity II VWD User Manual...

  • Page 63: Optimizing The Detector

    Agilent 1290 Infinity II VWD User Manual Optimizing the Detector Introduction Match the Flow Cell to the Column Set the Detector Parameters Warm up of the Detector This chapter provides information on how to optimize the detector. Agilent Technologies...

  • Page 64: Introduction

    The information below will guide you on how to get the best detector performance. Follow these rules as a start for new applications. It gives a rule- of- thumb for optimizing the detector parameters. Agilent 1290 Infinity II VWD User Manual...

  • Page 65: Match The Flow Cell To The Column

    • If longer columns (> 50 mm) for higher resolution are used, then the next larger flow cell is the preferred choice for higher sensitivity. Agilent 1290 Infinity II VWD User Manual...
  • Page 66 As a rule- of- thumb, the flow cell volume should be about 1/3 of the peak volume at half height. To determine the volume of your peaks, take the peak width as reported in the integration results multiply it by the flow rate and divide it by 3). Agilent 1290 Infinity II VWD User Manual...
  • Page 67 However you have to be aware that there is additional tolerance of gasket thickness and its N O T E compression ratio which is supposed to be very small in comparison with the machining tolerance. Agilent 1290 Infinity II VWD User Manual...

  • Page 68: Set The Detector Parameters

    • at a wavelength with moderate absorptivity if you work with high concentrations, and • preferably where the spectrum is flat for better linearity. 3 Consider to use time- programming to further optimization. Agilent 1290 Infinity II VWD User Manual...

  • Page 69: Warm Up Of The Detector

    The example below was done under stable environmental conditions. The figures below show the first two hours of a detector warm- up phase. The lamp was turned on immediately after turn on of the detector. Agilent 1290 Infinity II VWD User Manual...
  • Page 70 Optimizing the Detector Warm up of the Detector Figure 18 Detector Warm-up – 1 hour Figure 19 Detector Warm-up – 2 hour Agilent 1290 Infinity II VWD User Manual...

  • Page 71: Troubleshooting And Diagnostics

    Agilent 1290 Infinity II VWD User Manual Troubleshooting and Diagnostics Available Tests versus Interfaces Agilent Lab Advisor Software Overview about the troubleshooting and diagnostic features. Agilent Technologies...

  • Page 72: Available Tests Versus Interfaces

    • Preferred tool should be the Agilent Lab Advisor software, see “Agilent Lab Advisor Software” on page 73 • Screenshots used within these procedures are based on the Agilent Lab Advisor software. Figure 20 The Lab Advisor shows the available test Agilent 1290 Infinity II VWD User Manual...

  • Page 73: Agilent Lab Advisor Software

    The tests and diagnostic features that are provided by the Agilent Lab Advisor software may differ from the descriptions in this manual. For details, refer to the Agilent Lab Advisor software help files. Agilent 1290 Infinity II VWD User Manual...
  • Page 74 Troubleshooting and Diagnostics Agilent Lab Advisor Software Agilent 1290 Infinity II VWD User Manual...

  • Page 75: Error Information

    Agilent 1290 Infinity II VWD User Manual Error Information What Are Error Messages General Error Messages Timeout Shutdown Remote Timeout Lost CAN Partner Leak Leak Sensor Open Leak Sensor Short Compensation Sensor Open Compensation Sensor Short Fan Failed Open Cover...
  • Page 76 Agilent Lab Advisor Software Heater Power At Limit Cover Violation This chapter describes the meaning of detector error messages, and provides information on probable causes and suggested actions how to recover from error conditions. Agilent 1290 Infinity II VWD User Manual...

  • Page 77: What Are Error Messages

    In all cases, error propagation is done via the CAN bus or via an APG remote cable (see documentation for the APG interface). Agilent 1290 Infinity II VWD User Manual...

  • Page 78: General Error Messages

    An external instrument has generated a shutdown signal on the remote line. The module continually monitors the remote input connectors for status signals. A LOW signal input on pin 4 of the remote connector generates the error message. Agilent 1290 Infinity II VWD User Manual...

  • Page 79: Remote Timeout

    Exchange the remote cable. Defective remote cable. Check the instrument for defects (refer to the Defective components in the instrument instrument’s documentation). showing the not-ready condition. Agilent 1290 Infinity II VWD User Manual...

  • Page 80: Lost Can Partner

    Probable cause Suggested actions Ensure all fittings are tight. Loose fittings. Exchange defective capillaries. Broken capillary. Exchange flow cell components. Leaking flow cell. Agilent 1290 Infinity II VWD User Manual...

  • Page 81: Leak Sensor Open

    Probable cause Suggested actions Please contact your Agilent service Defective leak sensor. representative. Please contact your Agilent service Leak sensor incorrectly routed, being representative. pinched by a metal component. Agilent 1290 Infinity II VWD User Manual...

  • Page 82: Compensation Sensor Open

    Probable cause Suggested actions Please contact your Agilent service Defective power switch board representative. Please contact your Agilent service Loose connection between the power representative. switch board and the main board Agilent 1290 Infinity II VWD User Manual...

  • Page 83: Fan Failed

    Please contact your Agilent service The top foam was removed during representative. operation. Please contact your Agilent service Foam not activating the sensor. representative. Please contact your Agilent service Defective sensor or main board. representative. Agilent 1290 Infinity II VWD User Manual...

  • Page 84: Eri Messages

    The ERI (Enhanced Remote Interface) provides two error events related to over current situations on the +5 V and +24 V lines. Probable cause Suggested actions Reduce the load. The load on the ERI is too high. Agilent 1290 Infinity II VWD User Manual...

  • Page 85: Detector Error Messages

    Top foam removed while lamp is on. representative. Exchange the lamp. Defective or non-Agilent lamp. Please contact your Agilent service Defective main board. representative. Please contact your Agilent service Defective power supply. representative. Agilent 1290 Infinity II VWD User Manual...

  • Page 86: Uv Lamp: No Voltage

    Suggested actions Ensure the lamp is connected. Lamp disconnected. Exchange the lamp. Defective or non-Agilent lamp. Please contact your Agilent service Defective power supply. representative. Please contact your Agilent service Defective main board. representative. Agilent 1290 Infinity II VWD User Manual...

  • Page 87: No Heater Current

    Please contact your Agilent service Fan not running (permitting lamp on). representative. Please contact your Agilent service Defective main board. representative. Exchange the lamp. Defective or non-Agilent lamp. Please contact your Agilent service Defective power supply. representative. Agilent 1290 Infinity II VWD User Manual...

  • Page 88: Wavelength Calibration Setting Failed

    • Repeat the calibration. Current step value too far from maximum. • Please contact your Agilent service representative. Please contact your Agilent service Misaligned/defective grating assembly. representative. Please contact your Agilent service Defective main board. representative. Agilent 1290 Infinity II VWD User Manual...

  • Page 89: Wavelength Holmium Check Failed

    If defective, please contact your Agilent service representative. • Run the grating-motor test to determine if the grating assembly is defective. If defective, please contact your Agilent service representative. Agilent 1290 Infinity II VWD User Manual...

  • Page 90: Grating Or Filter Motor Errors

    Please contact your Agilent service Motor is not connected. representative. Please contact your Agilent service Defective motor. representative. Please contact your Agilent service Defective/missing grating or filter. representative. Please contact your Agilent service Cable/connector defective. representative. Agilent 1290 Infinity II VWD User Manual...

  • Page 91: Wavelength Test Failed

    Please contact your Agilent service Motor is not connected. representative. Please contact your Agilent service Defective motor. representative. Please contact your Agilent service Defective/missing grating or filter. representative. Please contact your Agilent service Cable/connector defective. representative. Agilent 1290 Infinity II VWD User Manual...

  • Page 92: Adc Hardware Error

    Probable cause Suggested actions Please contact your Agilent service Defective sensor or main board. representative. Verify that the ambient conditions are within Detector is exposed to illegal ambient the allowed range. conditions. Agilent 1290 Infinity II VWD User Manual...

  • Page 93: Illegal Temperature Value From Sensor At Air Inlet

    As a result the temperature control is switched off. Probable cause Suggested actions Please contact your Agilent service Defective connector or cable. representative. Please contact your Agilent service Defective heater. representative. Agilent 1290 Infinity II VWD User Manual...
  • Page 94 Probable cause Suggested actions Please contact your Agilent service The top foam was removed during representative. operation. Please contact your Agilent service Foam not activating the sensor. representative. Agilent 1290 Infinity II VWD User Manual...

  • Page 95: Test Functions

    Agilent 1290 Infinity II VWD User Manual Test Functions Introduction Intensity Test Intensity Test Evaluation Intensity Test Failed Cell Test Cell Test Failed Wavelength Verification-Calibration When to Calibrate the Detector ASTM Drift and Noise Test Quick Noise Test Dark Current Test...

  • Page 96: Introduction

    For details on the use of the interface refer to the interface documentation. The Lab Advisor shows the available test under Service & Diagnostics. Figure 21 The Lab Advisor shows the available test Agilent 1290 Infinity II VWD User Manual...

  • Page 97: Intensity Test

    Therefore, intensity spectra will differ slightly between instruments. The figure below shows a typical intensity test spectrum. The Intensity Test is available in Agilent Lab Advisor (preferred tool). Agilent 1290 Infinity II VWD User Manual...

  • Page 98: Intensity Test Evaluation

    1 Run the Intensity- Test with Agilent Lab Advisor (for further information see Online- Help of user interface). Figure 22 Intensity Test – Results Figure 23 Intensity Test – Signal Agilent 1290 Infinity II VWD User Manual...

  • Page 99: Intensity Test Failed

    Repeat the test with the flow cell removed. If Flow cell windows dirty the test passes, exchange the flow cell windows. Please contact your Agilent service Optics defect representative. Exchange the lamp. Defective lamp or optics. Agilent 1290 Infinity II VWD User Manual...

  • Page 100: Cell Test

    If the sample side shows much lower values than the reference side the flow cell might have a problem. Flush the flow cell with a flow of 1 mL/min for at least 10 minutes. Prerequisites Agilent 1290 Infinity II VWD User Manual...

  • Page 101: Cell Test Failed

    Online- Help of user interface). Figure 24 Test – Results Cell Test Failed Cell Test Failed Probable cause Suggested actions Flush flow cell Cell contaminated Clean/replace cell windows Cell windows are contaminated Check cell position Mechanical problem Agilent 1290 Infinity II VWD User Manual...

  • Page 102: Wavelength Verification-Calibration

    • after exchange of the optical unit or VWM board, • at a regular interval, at least once per year (for example, prior to an Operational Qualification/Performance Verification procedure), and • when chromatographic results indicate the detector may require recalibration. Agilent 1290 Infinity II VWD User Manual...
  • Page 103 Online- Help of user interface). Figure 25 Wavelength Calibration - Results If you select No, the test is aborted. If you select Yes, the re- calibration is performed (the offset is corrected). Agilent 1290 Infinity II VWD User Manual...

  • Page 104: Astm Drift And Noise Test

    1 Run the ASTM Drift and Noise Test with Agilent Lab Advisor (for further information see Online- Help of user interface). Figure 26 ASTM Drift and Noise Test – Results Figure 27 Drift and Noise Test – Signal Agilent 1290 Infinity II VWD User Manual...

  • Page 105: Quick Noise Test

    In order to obtain reliable results, the lamp should be turned on for at least 10 minutes prior to measurement. 1 Run the Quick Noise Test with Agilent Lab Advisor (for further information see Online- Help of user interface). Figure 28 Quick Noise Test – Results Agilent 1290 Infinity II VWD User Manual...
  • Page 106 Test Functions Quick Noise Test Figure 29 Quick Noise Test – Signal Agilent 1290 Infinity II VWD User Manual...

  • Page 107: Dark Current Test

    Next, the leakage current from both diodes is measured. 1 Run the Dark Current Test with the Agilent Lab Advisor (for further information see Online- Help of user interface). Figure 30 Dark Current Test - Results Agilent 1290 Infinity II VWD User Manual...

  • Page 108: Dark Current Test Failed

    Dark Current Test Failed Dark Current Test Failed Probable cause Suggested actions Replace optical unit. Defective sample or reference diode. Replace optical unit. Defective sample or reference ADC board. Exchange the main board. Defective main board. Agilent 1290 Infinity II VWD User Manual...

  • Page 109: Holmium Oxide Test

    “Declaration of Conformity for HOX2 Filter” on page 224. N O T E When to do the Test • after recalibration, • as part of the Operational Qualification/Performance Verification procedure, or • after flow cell maintenance or repair. Agilent 1290 Infinity II VWD User Manual...

  • Page 110: Interpreting The Results

    1 Run the Holmium Oxide Test with the Agilent Lab Advisor (for further information see Online- Help of user interface). Figure 32 Holmium Oxide Test - Results Figure 33 Holmium Oxide Test - Signals Agilent 1290 Infinity II VWD User Manual...

  • Page 111: Holmium Oxide Test Failed

    OK, exchange the flow cell components. Run the holmium oxide filter test. If the test Dirty or defective holmium oxide filter. fails, contact your Agilent service representative. Please contact your Agilent service Optical misalignment. representative. Agilent 1290 Infinity II VWD User Manual...

  • Page 112: D/A Converter (Dac) Test

    DAC test. When If the analog detector signal is noisy or missing. Preparations Lamp must be on for at least 10 minutes. Connect integrator, chart recorder or data system to the detector analog output. Agilent 1290 Infinity II VWD User Manual...
  • Page 113 The test can be started via the command line. 1 To start the test TEST: DAC 1 Reply: RA 00000 TEST:DAC 1 2 To stop the test TEST:DAC 0 Reply: RA 00000 TEST:DAC 0 Agilent 1290 Infinity II VWD User Manual...

  • Page 114: D/A Converter Test Evaluation

    The noise on the step should be less than 3 µV. Probable cause Suggested actions Check or replace the cable. Bad cable or grounding problem between detector and external device. Please contact your Agilent service Defective detector main board. representative. Agilent 1290 Infinity II VWD User Manual...

  • Page 115: Other Lab Advisor Functions

    Other Lab Advisor Functions Other Lab Advisor Functions EMFs - Early Maintenance Feature The EMFs screen allows you to view and manage the EMF counters for all modules in all systems. Figure 36 EMFs Agilent 1290 Infinity II VWD User Manual...
  • Page 116 Test Functions Other Lab Advisor Functions Agilent 1290 Infinity II VWD User Manual...

  • Page 117: Maintenance

    Agilent 1290 Infinity II VWD User Manual Maintenance Introduction to Maintenance Warnings and Cautions Overview of Maintenance Cleaning the Module Remove and Install the Doors Replace the Deuterium Lamp Replace the Flow Cell / Cuvette Holder Repairing the Flow Cells...

  • Page 118: Introduction To Maintenance

    Eye damage may result from directly viewing the UV-light produced by the lamp of the optical system used in this product. ➔ Always turn the lamp of the optical system off before removing it. Agilent 1290 Infinity II VWD User Manual...
  • Page 119 C A U T I O N ➔ If you connect external equipment to the instrument, make sure that you only use accessory units tested and approved according to the safety standards appropriate for the type of external equipment. Agilent 1290 Infinity II VWD User Manual...

  • Page 120: Overview Of Maintenance

    A pressure tightness test should be parts cleaning or cell windows. done after repair. exchange Leak sensor drying If leak has occurred. Check for leaks. Leak handling system If broken or corroded. Check for leaks. replacement Agilent 1290 Infinity II VWD User Manual...

  • Page 121: Cleaning The Module

    WA R N I N G hazard and damage the module ➔ Do not use an excessively damp cloth during cleaning. ➔ Drain all solvent lines before opening any connections in the flow path. Agilent 1290 Infinity II VWD User Manual...

  • Page 122: Remove And Install The Doors

    Press the release buttons and pull the front door out. For the Installation of the front door. Insert the hinges into their guides and move the door in until the release buttons click into their final position. Agilent 1290 Infinity II VWD User Manual...

  • Page 123: Replace The Deuterium Lamp

    C A U T I O N ➔ To prevent accidental electrostatic discharge when coming into contact with components inside the instrument, touch one of the metal housing panels at the front of the instrument. Agilent 1290 Infinity II VWD User Manual...
  • Page 124 Unscrew the two lamp screws (Pozidriv). Remove the lamp and place it on a clean place. N O T E Do not touch the glass bulb with your fingers. It may reduce the light output. Agilent 1290 Infinity II VWD User Manual...
  • Page 125 Turn the lamp ON and give the lamp more than 10 minutes to warm-up. Perform a Wavelength Re-calibration after lamp warm-up. After lamp on, the detector requires a warm-up time of 60 minutes. No measurements N O T E should be performed during this time. Agilent 1290 Infinity II VWD User Manual...

  • Page 126: Replace The Flow Cell / Cuvette Holder

    146 • “High Pressure Flow Cell 10 mm / 14 µL” on page 148 Preparations Turn the lamp OFF. Open the doors. Disconnect the inlet capillary and outlet tubing from the flow cell. Agilent 1290 Infinity II VWD User Manual...
  • Page 127 Fix the two thumb screws parallel and tight. center of the flow cell. Reconnect the inlet capillary and the outlet tubing. Close the doors. Perform a Wavelength Verification-Calibration to check the correct position of the flow cell/cuvette holder. Agilent 1290 Infinity II VWD User Manual...

  • Page 128: Repairing The Flow Cells

    “High Pressure Flow Cell 10 mm / 14 µL” on page 148 The shown cell parts will differ depending upon the flow cell type. For detailed parts N O T E schematics, refer to above mentioned pages. Agilent 1290 Infinity II VWD User Manual...
  • Page 129 Use adhesive tape to remove the peek ring, the window and the gasket. d Repeat step a through step c for the other window (keep the parts separate - otherwise they could be mixed!). Agilent 1290 Infinity II VWD User Manual...
  • Page 130 6 Perform a leak test. If OK, insert the flow cell. 7 Perform “Wavelength Verification- Calibration” on page 102 to check the correct positioning of the flow cell. 8 Replace the front cover. Agilent 1290 Infinity II VWD User Manual...

  • Page 131: Using The Cuvette Holder

    If your own standard should be used to checkout the instrument. Parts required Description G1314-60200 Cuvette Holder Cuvette with the “standard”, e.g. NIST certified holmium oxide sample Preparations • Remove the normal flow cell. • Have cuvette with standard available. Agilent 1290 Infinity II VWD User Manual...
  • Page 132 Next Steps: Install the cuvette holder in the instrument. Perform your Wavelength Verification/Calibration (see “Wavelength Verification-Calibration” on page 102) to check the correct position of the cuvette holder. Agilent 1290 Infinity II VWD User Manual...

  • Page 133: Correcting Leaks

    1 Open the doors. 2 Use tissue to dry the leak sensor area [1]. 3 Observe the capillary connections and the flow cell area [2] for leaks and correct, if required. Agilent 1290 Infinity II VWD User Manual...
  • Page 134 Maintenance Correcting Leaks 4 Close the doors. Agilent 1290 Infinity II VWD User Manual...

  • Page 135: Replacing Leak Handling System Parts

    Install the Leak Adapter by pressing it into the Main Cover. Insert the Tubing [2] (ca. 85 mm required for replacement) between Leak Adapter outlet and Leak Pan. Insert/close the doors. Agilent 1290 Infinity II VWD User Manual...

  • Page 136: Replace The Module Firmware

    1 Download the required module firmware, the latest LAN/USB FW Update Tool and the documentation from the Agilent web. http://www.chem.agilent.com/_layouts/agilent/downloadFirmware.aspx?whid=69761 2 For loading the firmware into the module follow the instructions in the documentation. Agilent 1290 Infinity II VWD User Manual...
  • Page 137 When using the G7114B in a system, all other modules must have firmware from set 6.50 or above (main and resident). Otherwise the communication will not work. Conversion / emulation Possible via Lab Advisor software: G1314E/F. Agilent 1290 Infinity II VWD User Manual...
  • Page 138 Maintenance Replace the Module Firmware Agilent 1290 Infinity II VWD User Manual...

  • Page 139: Parts And Materials For Maintenance

    Agilent 1290 Infinity II VWD User Manual Parts and Materials for Maintenance Overview of Maintenance Parts Standard Flow Cell 10 mm / 14 µL Micro Flow Cell 3 mm / 2 µL Semi-micro Flow Cell 6 mm / 5 µL High Pressure Flow Cell 10 mm / 14 µL...

  • Page 140: Overview Of Maintenance Parts

    Parts and Materials for Maintenance Overview of Maintenance Parts Overview of Maintenance Parts Figure 40 Overview of maintenance parts Agilent 1290 Infinity II VWD User Manual...
  • Page 141 5043-0856 Leak Adapter 5063-6527 Tubing assembly, i.d. 6 mm, o.d. 9 mm, 1.2 m (to waste) 5062-8535 Waste accessory kit (Flow Cell to Waste) 5067-5737 Door left 5067-5736 Door right 5043-1013 Tubing Clip Agilent 1290 Infinity II VWD User Manual...

  • Page 142: Standard Flow Cell 10 Mm / 14 Μl

    Gasket #1 kit (OUT large hole, i.d. 2.4 mm) KAPTON, 2/pk G1314-65065 Ring #1 kit (OUT large hole, i.d. 2.4 mm) PEEK, 2/pk G1314-44010 Clip for RFI ID tag 0515-4780 Screw for Clip, M2.2, 4.5 mm long Agilent 1290 Infinity II VWD User Manual...
  • Page 143 Parts and Materials for Maintenance Standard Flow Cell 10 mm / 14 µL Figure 41 Standard Flow Cell Agilent 1290 Infinity II VWD User Manual...

  • Page 144: Micro Flow Cell 3 Mm / 2 Μl

    Cell repair kit semi-micro, includes window screw kit, Gasket Kit BACK, Gasket Kit FRONT and 4 mm hexagonal wrench 79883-68703 Window screw kit, includes 2 quartz windows, 2 compression washers, 2 window holders, 2 window screws and 10 washers Agilent 1290 Infinity II VWD User Manual...
  • Page 145 3 - Compression washer 4 - Window holder 5 - Quartz window 6 - Gasket front 7 - Gasket back 2 (5x) 8 - RFID clip 9 - Screw Figure 42 Micro Flow Cell Agilent 1290 Infinity II VWD User Manual...

  • Page 146: Semi-Micro Flow Cell 6 Mm / 5 Μl

    Semi-micro #1 gasket (long hole 1.5 x 3.5 mm), PTFE Semi-micro #2 gasket (long hole 2 x 4 mm), PTFE G1314-44010 Clip for RFI ID tag 0515-4780 Screw for Clip, M2.2, 4.5 mm long Agilent 1290 Infinity II VWD User Manual...
  • Page 147 5 - Quartz window 6 - Semi-micro gasket #1 2 (3x) 7 - Semi-micro gasket #2 8 - Clip for RFI ID tag 9 - Screw for clip Figure 43 Semi-micro Flow Cell Agilent 1290 Infinity II VWD User Manual...

  • Page 148: High Pressure Flow Cell 10 Mm / 14 Μl

    Cell kit Agilent, comprises: two windows, two KAPTON gaskets and two PEEK rings Ring PEEK kit Window quartz kit Gasket kit, KAPTON G1314-44010 Clip for RFI ID tag 0515-4780 Screw for Clip, M2.2, 4.5 mm long Agilent 1290 Infinity II VWD User Manual...
  • Page 149 High Pressure Flow Cell 10 mm / 14 µL 1 - Cell screw 2 - Ring PEEK 3 - Quartz window 4 - Gasket KAPTON 5 - RFID Clip 6 - Screw Figure 44 High Pressure Flow Cell Agilent 1290 Infinity II VWD User Manual...

  • Page 150: Cuvette Holder

    Parts and Materials for Maintenance Cuvette Holder Cuvette Holder For information the use of the cuvette holder, refer to “Using the Cuvette Holder” on page 131. Description G1314-60200 Cuvette Holder Figure 45 Cuvette Holder Agilent 1290 Infinity II VWD User Manual...

  • Page 151: Accessory Kit

    5181-1516 CAN cable, Agilent module to module, 0.5 m 5500-1155 Tube Connector, 90 degree, ID 6.4 5043-1013 Tubing Clip (see item 7 in Figure 40 on page 140) 0100-1516 Fitting male PEEK, 2/pk Agilent 1290 Infinity II VWD User Manual...
  • Page 152 Parts and Materials for Maintenance Accessory Kit Agilent 1290 Infinity II VWD User Manual...

  • Page 153: Identifying Cables

    Agilent 1290 Infinity II VWD User Manual Identifying Cables Cable Overview Analog Cables Remote Cables CAN/LAN Cables RS-232 Cables USB Cables This chapter provides information on cables used with the Agilent 1200 Infinity Series modules. Agilent Technologies...

  • Page 154: Cable Overview

    Identifying Cables Cable Overview Cable Overview Never use cables other than the ones supplied by Agilent Technologies to ensure proper N O T E functionality and compliance with safety or EMC regulations. Analog cables Description 35900-60750 Agilent 35900A A/D converter...
  • Page 155 1-1, 2-3, 3-2, 4-6, 5-5, 6-4, 7-8, 8-7, 9-9. 5181-1561 RS-232 cable, 8 m USB cables Description 5188-8050 USB A M-USB Mini B 3 m (PC-Module) 5188-8049 USB A F-USB Mini B M OTG (Module to Flash Drive) Agilent 1290 Infinity II VWD User Manual...

  • Page 156: Analog Cables

    Agilent modules. The other end depends on the instrument to which connection is being made. Agilent Module to 35900 A/D converters p/n 35900-60750 35900 Pin Agilent Signal Name module Not connected Shield Analog - Center Analog + Agilent 1290 Infinity II VWD User Manual...
  • Page 157 Pin BNC Pin Agilent Signal Name module Shield Shield Analog - Center Center Analog + Agilent Module to General Purpose p/n 01046-60105 Pin Agilent Signal Name module Not connected Black Analog - Analog + Agilent 1290 Infinity II VWD User Manual...

  • Page 158: Remote Cables

    STOP READY POWER ON NOT USED SHUT DOWN START PREPARE 1wire DATA DGND +5V ERI out PGND PGND +24V ERI out +24V ERI out 5188- 8044 ERI to ERI (Connector D_Subminiature 15 pin) Agilent 1290 Infinity II VWD User Manual...
  • Page 159 5188- 8045 ERI to APG (Connector D_Subminiature 15 pin (ERI), Connector D_Subminiature 9 pin (APG)) p/n 5188-8045 Pin (ERI) Signal Pin (APG) Start Request Stop Ready Power on Future Shut Down Start Prepare Ground Cable Shielding Connection Agilent 1290 Infinity II VWD User Manual...
  • Page 160 Identifying Cables Remote Cables One end of these cables provides a Agilent Technologies APG (Analytical Products Group) remote connector to be connected to Agilent modules. The other end depends on the instrument to be connected to. Agilent Module to Agilent 35900 A/D Converters...
  • Page 161 Wire Color Pin Agilent Signal Name Active module (TTL) White Digital ground Brown Prepare run Gray Start Blue Shut down Pink connected Yellow Power on High Ready High Green Stop Black Start request Agilent 1290 Infinity II VWD User Manual...

  • Page 162: Can/Lan Cables

    CAN cable, Agilent module to module, 1 m LAN Cables Description 5023-0203 Cross-over network cable, shielded, 3 m (for point to point connection) 5023-0202 Twisted pair network cable, shielded, 7 m (for point to point connection) Agilent 1290 Infinity II VWD User Manual...

  • Page 163: Cables

    It's also called "Null Modem Cable" with full handshaking where the wiring is made between pins 1-1, 2-3, 3-2, 4-6, 5-5, 6-4, 7-8, 8-7, 9-9. 5181-1561 RS-232 cable, 8 m Agilent 1290 Infinity II VWD User Manual...

  • Page 164: Usb Cables

    To connect a USB Flash Drive use a USB OTG cable with Mini- B plug and A socket. Description 5188-8050 USB A M-USB Mini B 3 m (PC-Module) 5188-8049 USB A F-USB Mini B M OTG (Module to Flash Drive) Agilent 1290 Infinity II VWD User Manual...

  • Page 165: Hardware Information

    Agilent 1290 Infinity II VWD User Manual Hardware Information Firmware Description Electrical Connections Rear View of the Module Information on Instrument Serial Number Interfaces Overview Interfaces ERI (Enhanced Remote Interface) USB (Universal Serial Bus) Setting the 6-bit Configuration Switch Special Settings...

  • Page 166: Firmware Description

    • run synchronization through APG remote, • error handling, • diagnostic functions, • or module specific functions like • internal events such as lamp control, filter movements, • raw data collection and conversion to absorbance. Agilent 1290 Infinity II VWD User Manual...
  • Page 167 Update of main system can be done in the resident system only. Update of the resident N O T E system can be done in the main system only. Main and resident firmware must be from the same set. Agilent 1290 Infinity II VWD User Manual...
  • Page 168 All these specific informations are described in the documentation provided with the firmware update tools. The firmware update tools, firmware and documentation are available from the Agilent web. • http://www.chem.agilent.com/_layouts/agilent/downloadFirmware.aspx?whid=69761 Agilent 1290 Infinity II VWD User Manual...

  • Page 169: Electrical Connections

    There are no externally accessible fuses because automatic electronic fuses are implemented in the power supply. Never use cables other than the ones supplied by Agilent Technologies to ensure proper N O T E functionality and compliance with safety or EMC regulations.

  • Page 170: Rear View Of The Module

    • DE = Germany • JP = Japan • CN = China year and week of last major manufacturing change, e.g. 820 could be week 20 of 1998 or 2008 SSSSS real serial number Agilent 1290 Infinity II VWD User Manual...
  • Page 171 • CN = China Alphabetic character A-Z (used by manufacturing) Alpha-numeric code 0-9, A-Z, where each combination unambiguously denotes a module (there can be more than one code for the same module) 00000 Serial number Agilent 1290 Infinity II VWD User Manual...

  • Page 172: Interfaces

    (A) / ERI (E) Pumps G7104A Flexible Pump G7120A High Speed Pump Samplers G7129A/B ALS G7167A/B Multisampler Detectors G7114A/B VWD G7117A/B DAD G7115A/B DAD Others G7116B MCT Requires a HOST module via CAN Agilent 1290 Infinity II VWD User Manual...

  • Page 173: Overview Interfaces

    N O T E connected to the DAD/MWD/FLD/VWD/RID (due to higher data load). If no Agilent detector is part of the system, the LAN interface should be installed in the pump or autosampler. Agilent 1290 Infinity II VWD User Manual...
  • Page 174 Remote (ERI) The ERI (Enhanced Remote Interface) connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features as common shut down, prepare, and so on. It allows easy connection between single instruments or systems to ensure coordinated analysis with simple coupling requirements.
  • Page 175 PREPARE (L) Request to prepare for analysis (for example, calibration, detector lamp on). Receiver is any module performing pre-analysis activities. Special Interfaces There is no special interface for this module. Agilent 1290 Infinity II VWD User Manual...

  • Page 176: Eri (Enhanced Remote Interface)

    This way the interface can support various additional devices like sensors, triggers (in and out) and small controllers, etc. Figure 48 Location of the ERI interface (example shows a G7114A/B VWD) Agilent 1290 Infinity II VWD User Manual...
  • Page 177 This serial line can be used to read out an EPROM or write into an EPROM of a connected ERI- device. The firmware can detect the connected type of device automatically and update information in the device (if required). Agilent 1290 Infinity II VWD User Manual...

  • Page 178: Usb (Universal Serial Bus)

    • Short circuit will be detected through hardware. USB (Universal Serial Bus) USB (Universal Serial Bus) - replaces RS232, supports: • a PC with control software (for example Agilent Lab Advisor) • USB Flash Disk Agilent 1290 Infinity II VWD User Manual...

  • Page 179: Setting The 6-Bit Configuration Switch

    • For specific LAN modes switches 4- 5 must be set as required. • For boot resident/cold start modes switches 1+2 or 6 must be UP. Figure 49 Location of Configuration switch (example shows a G7114A/B VWD) Agilent 1290 Infinity II VWD User Manual...
  • Page 180 When selecting mode COM, settings are stored to non-volatile memory. When selecting mode TEST, COM settings are tak- en from non-volatile memory. not assigned - Always keep these switches on position ‘0’ (off) Default IP Address is 192.168.254.11 Host Name will be the MAC address. Agilent 1290 Infinity II VWD User Manual...

  • Page 181: Special Settings

    Exceptions are calibration settings, diagnosis and repair log books which will not be erased. ➔ Save your methods and data before executing a forced cold start. If you use the following switch settings and power the instrument up again, it will start as described above. Agilent 1290 Infinity II VWD User Manual...
  • Page 182 Boot Main System / Keep Data Boot Resident System / Keep Data Boot Main System / Revert to Default Data Boot Resident System / Revert to Default Data Note: The setting '0' (down) is essential. Agilent 1290 Infinity II VWD User Manual...

  • Page 183: Instrument Layout

    • the plastic layers help cushion the electronic and mechanical parts from physical shock, and • the metal inner cabinet shields the internal electronics from electromagnetic interference and also helps to reduce or eliminate radio frequency emissions from the instrument itself. Agilent 1290 Infinity II VWD User Manual...

  • Page 184: Early Maintenance Feedback

    The useful maintenance cycle is dependent on the requirements for use. Therefore, the definition of the maximum limits need to be determined based on the specific operating conditions of the instrument. Agilent 1290 Infinity II VWD User Manual...
  • Page 185 EMF counters to zero. The next time the EMF counters exceed the new EMF limits, the EMF flag will be displayed, providing a reminder that maintenance needs to be scheduled. This function is only available via Agilent Lab Advisor or Instant Pilot. N O T E Agilent 1290 Infinity II VWD User Manual...
  • Page 186 Hardware Information Early Maintenance Feedback Agilent 1290 Infinity II VWD User Manual...

  • Page 187: Lan Configuration

    Agilent 1290 Infinity II VWD User Manual LAN Configuration What You Have to Do First TCP/IP parameter configuration Configuration Switch Initialization Mode Selection Dynamic Host Configuration Protocol (DHCP) General Information (DHCP) Setup (DHCP) Manual Configuration With Telnet PC and Agilent ChemStation Setup...

  • Page 188: What You Have To Do First

    188) to • the PC network card using a crossover network cable (point- to- point) • a hub or switch using a standard LAN cable. Figure 51 Location of LAN interfaces and MAC label Agilent 1290 Infinity II VWD User Manual...

  • Page 189: Tcp/Ip Parameter Configuration

    TCP/IP parameters after power- on. The parameters may be derived from a Bootp cycle, non- volatile memory or initialized with known default values. The initialization mode is selected by the configuration switch, see Table 18 on page 191. Agilent 1290 Infinity II VWD User Manual...

  • Page 190: Configuration Switch

    To perform any LAN configuration, SW1 and SW2 must be set to OFF. N O T E Table 17 Factory Default Settings Initialization (‘Init’) Mode Bootp, all switches down. For details see “Initialization Mode Selection” on page 191 Agilent 1290 Infinity II VWD User Manual...

  • Page 191: Initialization Mode Selection

    They are not stored to the non- volatile memory of the module. Therefore, the parameters are lost with the next power cycle of the module. Figure 53 Bootp (Principle) Agilent 1290 Infinity II VWD User Manual...
  • Page 192 Use the initialization mode Bootp & Store carefully, because writing to the non-volatile N O T E memory takes time. Therefore, when the module shall obtain its parameters from a Bootp Server every time it is powered on, the recommended initialization mode is Bootp! Agilent 1290 Infinity II VWD User Manual...
  • Page 193 Figure 56 Using Default (Principle) Using the default address in your local area network may result in network problems. Take N O T E care and change it to a valid address immediately. Agilent 1290 Infinity II VWD User Manual...
  • Page 194 In the Using Default mode, the parameters stored in the memory of the module are not N O T E cleared automatically. If not changed by the user, they are still available, when switching back to the mode Using Stored. Agilent 1290 Infinity II VWD User Manual...

  • Page 195: Dynamic Host Configuration Protocol (Dhcp)

    2 It may be necessary to fully qualify the hostname with the DNS suffix, e.g. 0030d3177321.country.company.com. 3 The DHCP server may reject the hostname proposed by the card and assign a name following local naming conventions. Agilent 1290 Infinity II VWD User Manual...

  • Page 196: Setup (Dhcp)

    On the Instant Pilot the MAC address can be found under Details in the LAN section. Figure 58 LAN Setting on Instant Pilot 2 Set the Configuration Switch to DHCP either on the G1369C LAN Interface Card or the main board of above mentioned modules. Agilent 1290 Infinity II VWD User Manual...
  • Page 197 4 Configure your Control Software (e.g. Agilent ChemStation, Lab Advisor, Firmware Update Tool) and use MAC address as host name, e.g. 0030d3177321. The LC system should become visible in the control software (see Note in section “General Information (DHCP)” on page 195). Agilent 1290 Infinity II VWD User Manual...

  • Page 198: Manual Configuration

    Therefore, manual configuration can be done at any time. A power cycle is mandatory to make the stored parameters become the active parameters, given that the initialization mode selection switches are allowing it. Figure 59 Manual Configuration (Principle) Agilent 1290 Infinity II VWD User Manual...

  • Page 199: With Telnet

    When the connection was established successfully, the module responds with the following: Figure 61 A connection to the module is made 3 Type ? and press enter to see the available commands. Figure 62 Telnet Commands Agilent 1290 Infinity II VWD User Manual...
  • Page 200 Initialization mode is Using Stored active TCP/IP settings TCP/IP status - here ready connected to PC with controller software (e.g. Agilent Figure 63 Telnet - Current settings in "Using Stored" mode ChemStation), here not connected Agilent 1290 Infinity II VWD User Manual...
  • Page 201 If the Initialization Mode Switch is changed now to “Using Stored” mode, the instrument N O T E will take the stored settings when the module is re-booted. In the example above it would be 192.168.254.12. Agilent 1290 Infinity II VWD User Manual...

  • Page 202: Pc And Agilent Chemstation Setup

    This procedure describes the change of the TCP/IP settings on your PC to match the module’s default parameters in a local configuration (see Table 19 on page 194). 1 Open the Local Area Connection Properties and select Internet Protocol (TCP/IP). Then click on Properties. Agilent 1290 Infinity II VWD User Manual...
  • Page 203 LAN Configuration PC and Agilent ChemStation Setup 2 You may enter here the fixed IP address of the module or use the Alternative Configuration. Agilent 1290 Infinity II VWD User Manual...
  • Page 204 LAN Configuration PC and Agilent ChemStation Setup 3 We will use the direct LAN access via Cross- over LAN cable with the module’s IP address. 4 Click on OK to save the configuration. Agilent 1290 Infinity II VWD User Manual...

  • Page 205: Agilent Chemstation Setup

    The LAN must be connected to detector due to high data load on communication to Control N O T E Software. 1 Open the ChemStation Configuration Editor. 2 Select from the menu Configure - Instruments. 3 Select Modular 3D LC System. 4 Give the Instrument a name. Agilent 1290 Infinity II VWD User Manual...
  • Page 206 LAN Configuration PC and Agilent ChemStation Setup 5 Click on OK. 6 Select LC System Access — Access Point and click on Add. 7 Click on OK. Agilent 1290 Infinity II VWD User Manual...
  • Page 207 During first startup or when the system configuration has changed, a notification shows up. 12 The left column shows the modules that could be configured. You may select the module manually from the list. We use the Auto Configuration mode. Click on Yes. Agilent 1290 Infinity II VWD User Manual...
  • Page 208 The selected module is shown now in the right window (with serial number). In addition all other modules connected via CAN to the detector are shown as well. 15 Click on OK to continue the ChemStation loading. Agilent 1290 Infinity II VWD User Manual...
  • Page 209 Under Connection Settings you may change the IP/Hostname of the module (may require a re- start of the ChemStation). After successful load of the ChemStation, you should see the module(s) as active item in the graphical user interface (GUI). Agilent 1290 Infinity II VWD User Manual...
  • Page 210 LAN Configuration PC and Agilent ChemStation Setup Figure 66 Screen After Successful Load of ChemStation Agilent 1290 Infinity II VWD User Manual...

  • Page 211: Appendix

    Agilent 1290 Infinity II VWD User Manual Appendix General Safety Information General Safety Information Safety Standards General Before Applying Power Ground the Instrument Do Not Operate in an Explosive Atmosphere Do Not Remove the Instrument Cover Do Not Modify the Instrument...

  • Page 212: General Safety Information

    Agilent Technologies assumes no liability for the customer’s failure to comply with these requirements. Ensure the proper usage of the equipment.

  • Page 213: Before Applying Power

    Verify that the voltage range and frequency of your power distribution matches to the power specification of the individual instrument. ➔ Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations. ➔...

  • Page 214: Do Not Operate In An Explosive Atmosphere

    WA R N I N G Personal injury (for example electrical shock, intoxication) ➔ Instruments that appear damaged or defective should be made inoperative and secured against unintended operation until they can be repaired by qualified service personnel. Agilent 1290 Infinity II VWD User Manual...

  • Page 215: Solvents

    Check the filling level of the waste container regularly. ➔ To achieve maximal safety, check the correct installation regularly. ➔ Do not use solvents with an auto-ignition temperature below 200 °C (392 °F). Agilent 1290 Infinity II VWD User Manual...

  • Page 216: Safety Symbols

    European Community directives. The European Declaration of Conformity is available at: http://regulations.corporate.agilent.com/DoC/search.htm Manufacturing date. Power symbol indicates On/Off. The apparatus is not completely disconnected from the mains supply when the power switch is in the Off position Agilent 1290 Infinity II VWD User Manual...
  • Page 217 C A U T I O N alerts you to situations that could cause loss of data, or damage of equipment. ➔ Do not proceed beyond a caution until you have fully understood and met the indicated conditions. Agilent 1290 Infinity II VWD User Manual...

  • Page 218: Refrigerant

    Send for a doctor immediately. ➔ Moreover, it must be noted that HFKW-134a must always be extracted from the system and collected. It must never be discharged into the atmosphere on environmental grounds (greenhouse effect). Agilent 1290 Infinity II VWD User Manual...
  • Page 219 ➔ All media must be disposed of in accordance with national and local regulations. ➔ Please contact your local Agilent Service Center in regard to safe environmental disposal of the appliance. Agilent 1290 Infinity II VWD User Manual...

  • Page 220: Waste Electrical And Electronic Equipment Directive

    Monitoring and Control Instrumentation product. Do not dispose of in domestic household waste N O T E To return unwanted products, contact your local Agilent office, or see www.agilent.com more information. Agilent 1290 Infinity II VWD User Manual...

  • Page 221: Radio Interference

    Appendix Radio Interference Radio Interference Cables supplied by Agilent Technologies are screened to provide optimized protection against radio interference. All cables are in compliance with safety or EMC regulations. Test and Measurement If test and measurement equipment is operated with unscreened cables, or...

  • Page 222: Sound Emission

    This product has a sound pressure emission (at the operator position) < 70 dB. • Sound Pressure Lp < 70 dB (A) • At Operator Position • Normal Operation • According to ISO 7779:1988/EN 27779/1991 (Type Test) Agilent 1290 Infinity II VWD User Manual...

  • Page 223: Solvent Information

    THF, dioxane, di- isopropyl ether) such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides, • Solvents containing strong complexing agents (e.g. EDTA), • Mixtures of carbon tetrachloride with 2- propanol or THF. Agilent 1290 Infinity II VWD User Manual...

  • Page 224: Declaration Of Conformity For Hox2 Filter

    Appendix Declaration of Conformity for HOX2 Filter Declaration of Conformity for HOX2 Filter Agilent 1290 Infinity II VWD User Manual...

  • Page 225: Agilent Technologies On Internet

    Appendix Agilent Technologies on Internet Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at: http://www.agilent.com Agilent 1290 Infinity II VWD User Manual...
  • Page 226 Open Lab ChemStation heater power at limit detector warm up holmium oxide test failed overview ignition without cover RS-232 detector illegal temperature value from sensor preparation cables at air inlet analog DHCP Agilent 1290 Infinity II VWD User Manual...
  • Page 227 (parts) of LAN PC setup with RFID tag message flow cells ADC hardware error Bootp & Store matching for application calibration failed Bootp frequency range calibration lost cable filter check failed configuration switche Agilent 1290 Infinity II VWD User Manual...

  • Page 228: Product Description

    170, grating assembly power lamp set up considerations mirrors detector Agilent 1290 Infinity II VWD User Manual...
  • Page 229 TCP/IP parameter configuration equipment telnet wavelength configuration calibration temperature sensor WEEE directive test functions weight test function D/A converter introduction tests available tests vs interface holmium oxide intensity of deuterium lamp wavelength calibration Agilent 1290 Infinity II VWD User Manual...
  • Page 230 In This Book This manual contains technical reference information about the Agilent 1290 Infinity II Variable Wavelength Detector (G7114B). The manual describes the following: • introduction and specifications, • using and optimizing, • troubleshooting and diagnose, • maintenance, • parts identification, •...

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