Agilent Technologies 1260 Infinity II User Manual page 55

Solvent Recommendation for Agilent 1290 Infinity and 1290 Infinity II and 1260 Infinity II Flexible Pumps
• Use a minimum flow rate of 5 μL/min or 1 % composition per solvent
• When installing tubing connections to the MCGV, use lower channels (A/D)
• Consider using an inline filter, for example Inline Filter Assembly
• Never leave buffers in a system without flow. Before shutting down a
• Regularly maintain the LC system.
Handling of Acetonitrile
Acetonitrile is a solvent that is frequently used in reversed-phase
chromatography. Despite of its common use, it can be a source of issues if not
handled correctly. As Acetonitrile ages, some residue can get stuck on internal
pump surfaces which can cause issues with valve performance and therefore
affect retention time precision.
When using acetonitrile:
• Use high-quality solvents from renowned suppliers.
• Use fresh solvents and filter them.
• Minimize exposure to light and air/oxygen.
• Choose a bottle size which fits to your application and usage.
• Acids accelerate solvent aging. If possible avoid such additives or refresh
• Pure acetonitrile ages faster. If your application allows, add about 5 % water
• Do not leave acetonitrile in unused systems to avoid aging. If not in use,
• In case of blocked valves, flush the system with hot water. Knock at valves,
1260 Infinity II Flexible Pump User Manual
channel (whatever is greater) to avoid cross-flow. Cross-flow can be caused
by micro leaks in pump heads and can result in buffer precipitation in
pump heads, channel blocking, or reduced pump head life time through
wear of seals and pistons.
for aqueous solvents and upper channels for organic solvents. This will
re-dissolve precipitates more easily.
(5067-5407).
system, flush it extensively with warm water to avoid clogging of valves,
capillaries, or flow cells or reducing the life time of your column. If the
system is not used for some time, for example more than a day depending
on lab temperature, fill all solvent lines with organic solvent or water with
at least 10 % isopropanol.
solvents more frequently.
and adjust gradient compositions.
flush all solvent lines with a mixture of water and 10 % isopropanol.
flush them (see "Release a Stuck Inlet Valve"
them, for example in methanol.
Using the Module
on page 141) or ultrasonicate
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