Page 1 Diode Array Detectors Agilent InfinityLab LC Series User Manual Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 2 12.211 (Technical Data) and 12.212 (Computer Software) and, for the Depart- ment of Defense, DFARS 252.227-7015 (Technical Data - Commercial Items) and DFARS 227.7202-3 (Rights in Commer- cial Computer Software or Computer Software Documentation). Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 3 Overview about the troubleshooting and diagnostic features. Error Information This chapter describes the meaning of error messages, and provides information on probable causes and suggested actions how to recover from error conditions. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 4 This chapter describes the detector in more detail on hardware and electronics. LAN Configuration This chapter provides information on connecting the module to the Agilent ChemStation PC. Appendix This chapter provides addition information on safety, legal and web. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 5: Table Of Contents
Set up the Detector with Agilent Open Lab ChemStation The Detector User Interface Detector Control Settings Method Parameter Settings Preparing the Module Leak and Waste Handling Setting up an Analysis Solvent Information Capillary Coding Guide Installing Capillaries Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 6 Self-Test Intensity Test Cell Test Quick Noise Test ASTM Drift and Noise Test Slit Test Wavelength Verification Test Wavelength Calibration D/A Converter (DAC) Test Dark Current Test Other Lab Advisor Functions Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 7 Identifying Cables Cable Overview Analog Cables Remote Cables CAN/LAN Cables RS-232 Cables USB Cables Hardware Information Firmware Description Electrical Connections Interfaces Setting the 6-bit Configuration Switch Instrument Layout Early Maintenance Feedback Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 8 Initialization Mode Selection Dynamic Host Configuration Protocol (DHCP) Manual Configuration Appendix General Safety Information Waste Electrical and Electronic Equipment (WEEE) Directive Radio Interference Sound Emission Solvent Information Agilent Technologies on Internet Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 9: Introduction
Lamp Max-Light Cartridge Flow Cell Slit Assembly Grating and Diode Array Leak and Waste Handling Waste Concept Operating Principle This chapter gives an introduction to the detector and an instrument overview. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 10: Overview Of The Module
Improved Electronic temperature control (ETC) provides maximum baseline stability and practical sensitivity under fluctuating ambient temperature and humidity conditions. • Additional diagnostic signals for temperature and lamp voltage monitoring. • Easy exchange of flow cell by cartridge design. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 11: Product Description
For fast separations, this detector has multiple wavelength and full spectral detection at sampling rates up to 120 Hz. Status indicator Lamp housing cover Cartridge Flow Cell Power switch Leak drain Air inlet Figure 1 Overview of the detector Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 12: Product Description G7117B
For fast separations, this detector has multiple wavelength and full spectral detection at sampling rates up to 240 Hz. Status indicator Lamp housing cover Cartridge Flow Cell Power switch Leak drain Air inlet Figure 2 Overview of the detector Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 13: Product Description G7117C
For fast separations, this detector has multiple wavelength and full spectral detection at sampling rates up to 120 Hz. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 14: Optical System
Max-light cartridge flow cell at the other side and is focused by the fold mirror [4] through the slit assembly [5] onto a holographic grating [6] light being dispersed onto the diode array [7]. This allows simultaneous access to all wavelength information. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 15: Lamp
Nearly 100 % light transmission is achieved by utilizing total internal reflection in a non-coated silica fiber. Compromising refractive index and thermal effects are almost completely eliminated, resulting in significantly less baseline drift. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 16: Slit Assembly
The slit width is directly controlled by the micro-processor of the instrument and can be set as method parameter. Figure 7 Slit Assembly The slit width influences the spectral resolution and noise. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 17 The fixed slit combines the required optical functions - slit and shutter - in a simple and compact component. The slit width is fixed to 4 nm and both positions (fixed slit and shutter) are directly controlled by the micro- processor of the instrument. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 18: Grating And Diode Array
The diode array is a series of 1024 individual photodiodes and control circuits located on a ceramic carrier. It has a wavelength range from 190 – 640 nm and the sampling interval is 0.5 nm. Figure 9 Grating and diode array Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 19: Leak And Waste Handling
Introduction Leak and Waste Handling Leak and Waste Handling The Agilent InfinityLab LC Series has been designed for safe leak and waste handling. It is important that all security concepts are understood and instructions are carefully followed. The solvent cabinet is designed to store a maximum volume of 8 L solvent. The maximum volume for an individual bottle stored in the solvent cabinet should not exceed 2 L.
Page 20 Introduction Leak and Waste Handling Figure 10 Infinity II Leak Waste Concept (Flex Bench installation) Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 21 Introduction Leak and Waste Handling Figure 11 Infinity II Single Stack Leak Waste Concept (bench installation) Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 22 Infinity II Two Stack Leak Waste Concept (bench installation) The waste tube connected to the leak plane outlet on each of the bottom instruments guides the solvent to a suitable waste container. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 23: Waste Concept
1 Agilent recommends using the 6 L waste can with 1 Stay Safe cap GL45 with 4 ports (5043-1221) for optimal and safe waste disposal. If you decide to use your own waste solution, make sure that the tubes don't immerse in the liquid. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 24: Operating Principle
Introduction Operating Principle Operating Principle Flow cell cartridge Column Waste Figure 13 Hydraulic path Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 25: Site Requirements And Specifications
Site Requirements and Specifications Site Requirements Physical Specifications Performance Specifications Specifications Specification Conditions This chapter provides information on environmental requirements, physical and performance specifications. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 26: Site Requirements
Make sure the power connector of the instrument can be easily reached  and unplugged. Provide sufficient space behind the power socket of the instrument to  unplug the cable. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 27 Never use a power cord other than the one that Agilent shipped with this  instrument. Never use the power cords that Agilent Technologies supplies with this  instrument for any other equipment. Never use cables other than the ones supplied by Agilent Technologies ...
Page 28 This module is designed to operate in a typical electromagnetic environment, i.e. NOT E where RF transmitters such as mobile telephones may not be used in close proximity. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 29: Physical Specifications
Up to 3000 m (9842 ft) Safety standards: IEC, EN, Overvoltage category II, Pollution degree 2 For indoor use only. CSA, UL ISM Classification ISM Group 1 Class B According to CISPR 11 Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 30: Performance Specifications
2 – 400 nm, programmable in 1 nm steps Slit width 4 nm Diode width ~0.5 nm Time programmable Wavelength, polarity, peak width, lamp bandwidth, auto balance, wavelength range, threshold, spectra storage mode Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 31 Maximum operating pressure (MOP): Maximum pressure at which a system can operate continuously un- der normal conditions. Maximum incidental pressure (MIP): The maximum pressure which the system can experience during a short time. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 32 = 0.9 µL  Max-Light Cartridge Cell LSS: 10 mm, = 1.0 µL Maximum Operating Pressure (MOP) : 70 bar (60 bar for LSS) Maximum Incidental Pressure (MIP) : 150 bar Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 33 Maximum operating pressure (MOP): Maximum pressure at which a system can operate continuously un- der normal conditions. Maximum incidental pressure (MIP): The maximum pressure which the system can experience during a short time. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 34 Spectral tools Data analysis software for spectra evaluation, including spectral libraries and peak purity functions. Analog output Recorder/integrator: 100 mV or 1 V, output range 0.001 – 2 AU, one output Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 35 Maximum operating pressure (MOP): Maximum pressure at which a system can operate continuously un- der normal conditions. Maximum incidental pressure (MIP): The maximum pressure which the system can experience during a short time. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 36: Specification Conditions
Time Constant versus Response Time According to ASTM E1657-98 „Standard Practice of Testing Variable-Wavelength Photometric Detectors Used in Liquid Chromatography” the time constant is converted to response time by multiplying by the factor 2.2. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 37: Using The Module
Instrument Configuration Set up the Detector with Agilent Open Lab ChemStation The Detector User Interface Detector Control Settings Method Parameter Settings This chapter explains the essential operational parameters of the module. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 38: Magnets
Using the Module Magnets Magnets Magnets in doors of pumps, autosamplers, detectors, and fraction collectors. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 39: Turn On/Off
Using the Module Turn on/off Turn on/off This procedure exemplarily shows an arbitrary LC stack configuration. Power switch: On Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 40 Using the Module Turn on/off Turn instrument On/Off with the control software. Power switch: Off Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 41: Status Indicators
5. Resident mode (blinking) - for example, during update of main firmware. 6. Bootloader mode (fast blinking). Try to re-boot the module or try a cold-start. Then try a firmware update. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 42: Instrument Configuration
All switches DOWN: module uses the default IP address 192.168.254.11. b Switch 4 UP and others DOWN: module uses DHCP. c Switch 5 UP and others DOWN: modules uses STORED address. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 43 Using the Module Instrument Configuration 2 Enter the setup information (MAC / IP address and/or Instrument Name). a Agilent OpenLab ChemStation (Configure Instrument): b Lab Advisor (Instrument Overview - Add Instrument): Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 44: Set Up The Detector With Agilent Open Lab Chemstation
ChemStation Method and Run Control (just detector is shown) After successful load of the OpenLab ChemStation, you should see the module as an active item in the graphical user interface (GUI). Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 45: The Detector User Interface
EMF warning. Maintenance required (red) Important: The EMF settings can be accessed via Agilent Lab Advisor. The limit(s) can be changed. Based on the limit, the User Interface displays the above status. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 46 • Set Error Method • Identify Module (Status LED will blink) • Perform a Balance • Switch the UV-lamp on/off (same as click on button “Make Device Ready/Turn device off (standby)”) Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 47: Detector Control Settings
The correct selection is import- ant for optimal performance and lifetime. • At Power On: automatic lamp-on at power on. • Automatic Turn On: automatic detector power Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 48: Method Parameter Settings
Figure 15 Method parameter settings For additional help and support: Highlight the desired cell and press F1. A help NOT E screen will open with additional information and documentation about the topic. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 49 The G7117A and the G7117C have a data rate of up to 120 Hz. Figure 17 G7117A/C Peakwitdth Settings The G7117B has a data rate of up to 240 Hz. For details see “Peak width (response time)” on page 85. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 50 If unchecked, the lamp will be turned off after the analysis has finished. Note that the lamp on requires at least one hour warm-up time, see “Warm up of the Detector” on page 102. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 51 These signals can be used in addition to the normal baseline signal to determine whether correlation to temperature or volt- age/current of the lamp. These signals are available via the Agilent ChemStation Online Plot/Data Signal and/or Agilent Lab Advisor Software. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 52: Preparing The Module
Capillary Coding Guide Syntax for Capillary Description At-a-Glance Color-Coding Keys Installing Capillaries This chapter provides information on how to set up the module for an analysis and explains the basic settings. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 53: Leak And Waste Handling
Regularly check the filling level of the waste container. The residual free  volume in the waste container must be large enough to collect the waste liquid. To achieve maximal safety, regularly check the tubing for correct  installation. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 54: Waste Concept
1 Agilent recommends using the 6 L waste can with 1 Stay Safe cap GL45 with 4 ports (5043-1221) for optimal and safe waste disposal. If you decide to use your own waste solution, make sure that the tubes don't immerse in the liquid. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 55: Setting Up An Analysis
(when available in the system) and the pump. Solvents containing volatile ingredients will slightly lose these. Therefore priming of the pumping system is required before starting an application. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 56 2 Flush all tubes with at least 30 mL of solvent. 3 Set flow to required value of your application and close the purge valve. Pump for approximately 10minutes before starting your application. NOT E Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 57: Requirements And Conditions
Table 8 Conditions Flow 1.5 mL/min Stoptime 8 min Solvent 100% (30% water/70% Acetonitrile) Temperature Ambient Wavelength sample 254 nm Injection Volume 1 µL Column Temperature (optional): 25 °C or ambient Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 58 Variations in solvent quality, column packing, standard concentration and column temperature will all have a potential effect on peak retention and response. Figure 18 Typical Chromatogram with UV-detector Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 59: Preparing The Detector
Solvents (fill water and Acetontrile into the solvent bottles). 5 Load the default method. 6 Pump the water/acetonitrile (30/70 %) mobile phase through the column for 10 min for equilibration. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 60 Preparing the Module Setting up an Analysis 7 Select the menu item Run Control > Sample Info and enter information about this application. Click OK to leave this screen. Figure 19 Sample Info Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 61: Running The Sample And Verifying The Results
Figure 20 Chromatogram with Isocratic Test Sample Information about using the Data Analysis functions can be obtained from the NOT E Using your ChemStation manual supplied with your system. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 62: Solvent Information
Most data available refers to room temperature (typically 20 – 25 °C, 68 – 77 °F). If corrosion is possible, it usually accelerates at higher temperatures. If in doubt, please consult technical literature on chemical compatibility of materials. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 63 UHPLC and biochemical instrumentation. It is stable in the specified pH range (for the Bio-Inert LC system: pH 1 – 13, see bio-inert module manuals for details), and inert to many common solvents. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 64 It can be corroded by strong bases (e.g. hydroxide solutions > 10 %, diethylamine). It is not recommended for the use with fluoric acid and fluorides. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 65 For example, a 1 % solution of acetic acid in methanol will attack steel. • Solutions containing strong complexing agents (for example, EDTA, ethylene diamine tetra-acetic acid). • Mixtures of carbon tetrachloride with isopropanol or THF. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 66 It can be corroded by complexing cyanides and concentrated acids like aqua regia. Zirconium Oxide (ZrO Zirconium Oxide is inert to almost all common acids, bases and solvents. There are no documented incompatibilities for HPLC applications. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 67 Sapphire, Ruby and Al -based ceramics Sapphire, ruby and ceramics based on aluminum oxide Al are inert to almost all common acids, bases and solvents. There are no documented incompatibilities for HPLC applications. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 68: Capillary Coding Guide
Port id Tube Tubing FS/PK PEEK-coat Metric M3 ed fused + 1.6 mm Port id silica Heat Heat PK/SS Stainless Swagelok exchanger exchanger steel-coat union ed PEEK PFFE PTFE Long Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 69 MP35N Nickel-cob Long head alt-chro- mium-mol ybdenium alloy Small head SW 4 Small head SW 5 Fin- ger-tight 1200 bar PEEK Intermedi- Fused silica in contact with solvent Stainless steel-coated PEEK Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 70: At-A-Glance Color-Coding Keys
Bone White As you move to smaller-volume, high efficiency columns, you’ll want to use H INT narrow id tubing, as opposed to the wider id tubing used for conventional HPLC instruments Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 71: Installing Capillaries
Capillary ST, 0.12 mm x 500 mm SL/S (5500-1157) Multisampler MCT Valve/Heat Exchanger Capillary ST 0.12 mmX 400 mm SL/SL (5500-1251) Vialsampler MCT Valve/Heat Exchanger Capillary ST 0.12 mm x 105 mm SL/SL (5500-1238) Vialsampler ICC Heat Exchanger Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 72 1.5 m and PEEK Fittings 10/PK (p/n 5063-6591) (G5615-68755) Waste accessory kit (5062-8535) Waste Tube PTFE 0.7 mm x 5 m, 1.6 mm od (5062-2462) DAD/FLD Waste Analytical tubing kit 0.25 mm i.d. PTFE-ESD (G5664-68712) Detector Fraction Collector Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 73 “Syntax for Capillary Description” on page 68. Select a nut that is long enough for the fitting you'll be Slide the nut over the end of the tubing or capillary. using. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 74 If changing from a column or an injection valve to another, the fitting may leak or decrease the quality of the separation by contributing to band broadening. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 75: Optimizing The Detector
Delay Volume and Extra-Column Volume How to Configure the Optimum Delay Volume How to Achieve Higher Sensitivity Warm up of the Detector This chapter provides information on how to optimize the detector. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 76: Introduction
How to Get the Best Detector Performance The information below will guide you on how to get the best detector performance. Follow these rules as a start for new applications. It gives rules-of-thumb for optimizing detector parameters. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 77: Optimization Overview
Set the spectral wavelength range (for colorless samples 190...400 nm is sufficient). • Set step to 4 nm for normal use; set small step (and slit width) if high resolu- tion of spectra with fine structure is wanted. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 78: Choosing A Flow Cell
G7117B DAD. The cell is a requirement for the Ultra-Low Dispersion Kit solution which currently exists as 1290 Infinity Ultra-Low Dispersion Kit (5067-5189). The cell should be part of the ultra-low dispersion solution. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 79 The use of Peek-FS capillaries is not recommended. In combination with the SST zero dead volume fitting (e.g. at the inlet) the capillary could break and the glass particles could block/damage the flow cell. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 80 When performing detector tests in Lab Advisor, remove the Aperture. Aperture is not compatible with other Max-Light Cartridges. It can only be installed on the Max-Light Cartridge Cell LSS ( 10 mm, V(σ) 1.0 µL) (G7117-60020). Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 81: Inline Pressure Relief Valve Kit (G4212-68001)
The pressure relief valve is designed to protect the Max-Light Cartridge Flow Cell. Agilent Technologies strongly recommend installing the pressure relief valve at the outlet of the detector as soon as a second detector is installed like in LC/MS applications.
Page 82 The linearity limit of the detector is seen at about 2 AU for both, the 10 mm and the 60 mm Max-Light Cartridge Flow Cell. Using firmware revision B.06.25 and below, the 60 mm Max-Light Cartridge Cell linearity limit would be 333 mAU/cm. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 83: Optimizing For Sensitivity, Selectivity, Linearity And Dispersion
When increasing the path length, the cell volume could increase. Depending on the peak volume, this could cause more peak dispersion. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 84 Agilent detector it is necessary to have more precise information on path lengths of the detector flow cells.  Part Number Path Length Cell Volume ( G4212-60008/G5615-60018 1.0 cm 1.0 µL G4212-60007/G5615-60017 6.0 cm 4.0 µL Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 85: Peak Width (Response Time)
(over-filtering) will reduce peak height by about 20 % and reduce baseline noise by a factor of 1.4. This gives you the best possible signal-to-noise ratio, but may affect peak resolution. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 86 [min] pts/scan pts/scan pts/scan < 0.0015625 0.015625 > 0.0015625 0.03125 > 0.003125 0.0625 > 0.00625 0.125 > 0.0125 0.25 > 0.025 > 0.05 > 0.1 > 0.2 1.25 1.25 1.25 1.25 Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 87: Sample And Reference Wavelength And Bandwidth
The signals comprises a series of data points over time, with the average absorbance in the sample wavelength band minus the average absorbance of the reference wavelength band. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 88 2.5, whereas the signal is about 75 % of a 4 nm wide band. The signal-to-noise ratio for a 30 nm bandwidth is twice that for a 4 nm bandwidth in our example. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 89 This is almost completely eliminated by using a reference wavelength. With this technique, PTH-amino acids can be quantified in the low picomole range even in a gradient analysis. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 90: Slit Width (G7117B)
A narrow slit provides spectral resolution for analytes with very fine structures in the absorbance spectrum. An example of such a spectrum is benzene. The five main absorbance bands (fingers) are only 2.5 nm wide and just 6 nm apart from each other. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 91 Any photodiode receives light within a range of wavelength determined by the slit width. This explains why the fine spectral structure of benzene disappears when using a 8 nm wide slit. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 92: Optimizing Spectral Acquisition
For anisic acid (the example used before) a step of 4 nm would be sufficient. However a step of 2 nm gives a more optimal display of the spectrum. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 93: Margin For Negative Absorbance
Only for such cases, increase the margin for negative absorbance to avoid overflow of the analog-to-digital converter. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 94: Optimizing Selectivity
WL1 (204 nm, caffeine) WL2 (222 nm, hydrochlorothiazide) WL3 (260 nm, reference to suppress) hydrochlorothiazide) WL4 (282 nm, reference to suppress caffeine) Wavelength (nm) Figure 28 Wavelength Selection for Peak Suppression Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 95 No reference No reference Hydrochlorothiazides Caffeine suppressed uppressed Wavelength 204 nm Wavelength 222 nm Reference 260 nm Reference 282 nm Time (min) Time (min) Figure 29 Peak Suppression Using Reference Wavelength Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 96: Optimizing The Detector Regarding To The System
The extra-column volume is minimized with the Agilent InfinityLab LC Series system due to the narrow-bore (0.12 mm i.d.) tubing, the low-volume heat exchangers in the column compartment and the flow cell in the detector.
Page 97: How To Achieve Higher Sensitivity
For example, a signal at wavelength 250 nm with a bandwidth of 16 nm will be an average of the absorbance data from 242 nm to 258 nm. Additionally, a reference wavelength and reference bandwidth can be defined for each signal. The average Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 98 This is shown in the spectrum for anisic acid in Figure 30 on page 99. This spectrum is typical of many small molecules containing a UV chromophore. For best results the reference has been set so that Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 99 The detector internally acquires data points faster than is needed for a chromatogram and processes them to produce the signal seen by the data system. Part of the processing reduces the data to an appropriate data rate Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 100 [HZ] height [min] ≤126 pts/scan ≤251 pts/scan ≤501 pts/scan >501 pts/scan < 0.00078125 0.0078125 > 0.00078125 0.015625 > 0.0015625 0.03125 > 0.003125 0.0625 > 0.00625 0.125 > 0.0125 0.25 > 0.025 Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 101 100 and Table 20 on page 101. Running at 240, the spectra scan data rate is reduced automatically if the spectra scan data rate is more than 251 points/scan. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 102: Warm Up Of The Detector
The example below was done under stable environmental conditions. The figures below show the first two hours of a detector warm-up phase. The lamp was turned on immediately after turn on of the detector. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 103 Optimizing the Detector Warm up of the Detector Figure 31 Detector Warm-up – 1 hour Figure 32 Detector Warm-up – 2 hour Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 104: Troubleshooting And Diagnostics
Troubleshooting and Diagnostics Available Tests vs User Interfaces Agilent Lab Advisor Software Overview about the troubleshooting and diagnostic features. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 105: Available Tests Vs User Interfaces
Preferred tool should be the Agilent Lab Advisor software, see “Agilent Lab Advisor Software” on page 107. • Screenshots used within these procedures are based on the Agilent Lab Advisor software. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 106 Troubleshooting and Diagnostics Available Tests vs User Interfaces Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 107: Agilent Lab Advisor Software
The tests and diagnostic features that are provided by the Agilent Lab Advisor software may differ from the descriptions in this manual. For details, refer to the Agilent Lab Advisor software help files. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 108: Error Information
Heater at fan assembly failed Heater Power At Limit This chapter describes the meaning of error messages, and provides information on probable causes and suggested actions how to recover from error conditions. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 109: What Are Error Messages
In all cases, error propagation is done via the CAN bus or via an APG/ERI remote cable (see documentation for the APG/ERI interface). Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 110: General Error Messages
Check the vacuum degasser for an error condi- The degasser failed to generate sufficient tion. Refer to the Service Manual for the degasser vacuum for solvent degassing. or the pump that has the degasser built-in. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 111: Remote Timeout
Exchange the CAN cable. Defective CAN cable. Switch off the system. Restart the system, and Defective mainboard in another module. determine which module or modules are not rec- ognized by the system. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 112: Leak Sensor Short
Defective leak sensor. tive. Please contact your Agilent service representa- Leak sensor incorrectly routed, being tive. pinched by a metal component. Please contact your Agilent service representa- Power switch assembly defective tive. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 113: Compensation Sensor Open
Probable cause Suggested actions Please contact your Agilent service representa- Defective power switch assembly tive. Please contact your Agilent service representa- Loose connection between the power switch tive. board and the mainboard Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 114: Fan Failed
This changes the resistance of the leak sensor which is sensed by the leak sensor circuit on the main board. Probable cause Suggested actions Ensure all fittings are tight. Loose fittings. Exchange defective capillaries. Broken capillary. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 115: Open Cover
The ERI (Enhanced Remote Interface) provides two error events related to over current situations on the +5 V and +24 V lines. Probable cause Suggested actions Reduce the load. The load on the ERI is too high. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 116: Detector Error Messages
Exchange the UV lamp. Defective UV lamp or non-Agilent lamp. Please contact your Agilent service representa- Defective detector main board. tive. Please contact your Agilent service representa- Defective power supply. tive. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 117: Uv Lamp Voltage
Exchange the UV lamp. Defective UV lamp or non-Agilent lamp. Please contact your Agilent service representa- Defective detector main board. tive. Please contact your Agilent service representa- Defective power supply. tive. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 118: Uv Heater Current
ROM, the error message is generated. Probable cause Suggested actions Please contact your Agilent service representa- Defective connector or cable. tive. Please contact your Agilent service representa- Defective PDA/optical unit. tive. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 119: Wavelength Recalibration Lost
Suggested actions Please contact your Agilent service representa- Defective sensor or main board. tive. Verify that the ambient conditions are within the Detector is exposed to illegal ambient condi- allowed range. tions. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 120: Illegal Temperature Value From Sensor At Air Inlet
0 means upper power limit hit (excessive ambient temperature drop). 1 means lower power limit hit (excessive ambient temperature increase). Probable cause Suggested actions Wait until temperature control equilibrates. Excessive ambient temperature change. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 121: Test Functions And Calibration
Wavelength Recalibration Fails D/A Converter (DAC) Test Test Evaluation Dark Current Test Test Failed Other Lab Advisor Functions EMFs - Early Maintenance Feature This chapter describes the tests for the module. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 122: Introduction
Advanced - Quick Noise Test Basic Advanced - Self Test Basic Advanced - Slit Test (G7117B only) Basic Advanced - Wavelength Verification Test Basic Advanced Calibrations - Wavelength Calibration Basic Advanced Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 123 - Method Parameters - Set Signal C Advanced - Set Signal B Advanced - Set Signal A Advanced - Set Data Rate [Hz] Advanced - Module Information - Identify Module Basic Advanced Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 124 - Board Temperature [°C] Advanced - Optical Temperature [°C] Advanced EMF Counters - Accumulated UV Lamp On-Time Basic Advanced - Number of UV Lamp Ignitions Basic Advanced - Current UV Lamp On-Time Basic Advanced Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 125 Test Functions and Calibration Introduction Figure 33 The Lab Advisor shows the available test Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 126: Use Of Max-Light Cartridge Test Cell
) 0.4 µL)  G4212-60017 Max-Light Cartridge Cell ULD (10 mm, V( 100 % ) 0.6 µL) G7117-60020 Max-Light Cartridge Cell LSS (10 mm, V(  ~ 100 % ) 1.0 µL) Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 127: Conditions Of Detector
If a test fails with the Max-Light Cartridge Cell repeat the test with the Max-Light Cartridge Test Cell and compare. If the test fails also, then start with proposed actions mentioned in the details of the tests. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 128: Self-Test
• For noise test a longer warm-up time may be required (> 2 hours). • When using a Max-Light Cartridge Cell a flow rate of 0.5 mL/min with water is required. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 129 1 Run the Self-Test with Agilent Lab Advisor (for further information see Online-Help of user interface). Figure 34 Self-Test – Results Under the tab Signals you can find the detailed signals from the tests. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 130: Intensity Test
Lamp must be on for at least 10 minutes. 1 Run the Intensity-Test with Agilent Lab Advisor (for further information see Online-Help of user interface). Figure 35 Intensity Test – Results Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 131: Test Failed
Run the cell test. If the test fails, flush the flow Dirty or contaminated flow cell. cell. See also “Clean the Max-Light Cartridge Cell” on page 165. Please contact your Agilent service representa- Dirty or contaminated optical components. tive. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 132: Cell Test
Max-Light Cartridge Test Cell Preparations • Lamp must be on for at least 10 minutes. • When using a Max-Light Cartridge Cell a flow rate of 0.5 mL/min with water is required. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 133: Test Failed (Low Ratio Value)
Absorbing solvent or air bubble in flow cell. from air bubbles. Clean the flow cell as described in “Clean the Dirty or contaminated flow cell. Max-Light Cartridge Cell” on page 165. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 134: Quick Noise Test
Detector and UV-lamp must be on for at least 2 hours. • ASTM measurements based on specifications may require longer stabilization times. • When using a Max-Light Cartridge Cell a flow rate of 0.5 mL/min with water is required. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 135 1 Run the Quick Noise Test with Agilent Lab Advisor (for further information see Online-Help of user interface). Figure 39 Quick Noise Test – Results Figure 40 Quick Noise Test – Signal Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 136: Test Failed
• Flush flow cell. Dirty or contaminated flow cell. • Clean the flow cell as described in “Clean the Max-Light Cartridge Cell” on page 165. Exchange the UV-lamp. Old UV-lamp. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 137: Astm Drift And Noise Test
Detector and UV-lamp must be on for at least 2 hours. • ASTM measurements based on specifications may require longer stabilization times. • When using a Max-Light Cartridge Cell a flow rate of 0.5 mL/min with water is required. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 138 1 Run the ASTM Drift and Noise Test with Agilent Lab Advisor (for further information see Online-Help of user interface). Figure 41 ASTM Drift and Noise Test – Results Figure 42 ASTM Drift and Noise Test – Signal Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 139: Test Failed
Dirty or contaminated flow cell. • Clean the flow cell as described in “Clean the Max-Light Cartridge Cell” on page 165. Exchange the UV-lamp. Old UV-lamp. Environment not according to specifications. Improve environment. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 140: Slit Test
When using a Max-Light Cartridge Cell a flow rate of 0.5 mL/min with water is required. 1 Run the Slit Test with the Agilent Lab Advisor (for further information see Online-Help of user interface). Figure 43 Slit Test – Results Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 141: Test Failed
Please contact your Agilent service representa- Defective slit assembly. tive. Please contact your Agilent service representa- Defective detector main board. tive. Please contact your Agilent service representa- Defective optical unit. tive. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 142: Slit Test (G7117A/G7117C)
There is no dedicated slit test for the G7117A/G7117C. To verify the proper function perform the following tests: • Intensity Test (tests the normal position) • Dark Current Test (tests the dark position) Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 143: Wavelength Verification Test
Max-Light Cartridge Cell Preparations • Lamp must be on for at least 10 minutes. • When using a Max-Light Cartridge Cell a flow rate of 0.5 mL/min with water is required. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 144 Test Functions and Calibration Wavelength Verification Test 1 Run the Wavelength Verification Test with the Agilent Lab Advisor (for further information see Online-Help of user interface). Figure 45 Wavelength Verification – Results Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 145: Wavelength Calibration
If the detector was repaired (opened covers), the wavelength calibration can be NOT E done 10 minutes after lamp on. A final wavelength calibration should be repeated after complete warm-up of the detector. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 146 Online-Help of user interface). Figure 46 Wavelength Calibration - Results If you select No, the test is aborted. If you select Yes, the re-calibration is performed (the offset is corrected). Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 147: Wavelength Recalibration Fails
See also “Clean the Max-Light Cartridge Cell” on page 165. If the test fails with Max-Light Cartridge Test Cell and new UV-lamp, the optical NOT E unit must be replaced. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 148: D/A Converter (Dac) Test
1 Run the D/A Converter (DAC) Test with the Agilent Lab Advisor (for further information see Online-Help of user interface). Figure 47 D/A Converter (DAC) Test – Results Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 149: Test Evaluation
The noise on the step should be less than 3 µV. Probable cause Suggested actions Check or replace the cable. Bad cable or grounding problem between detector and external device. Please contact your Agilent service representa- Defective detector main board. tive. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 150: Dark Current Test
1 Run the Dark Current Test with the recommended user interface (for further information see Online-Help of user interface). Figure 49 Dark Current Test – Results Figure 50 Dark Current Test – Signals Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 151: Test Failed
140 (part of the “Self-Test” on page 128). Please contact your Agilent service representa- Defective detector main board. tive. Please contact your Agilent service representa- Defective PDA/optical unit. tive. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 152: Other Lab Advisor Functions
Other Lab Advisor Functions Other Lab Advisor Functions EMFs - Early Maintenance Feature The EMFs screen allows you to view and manage the EMF counters for all modules in all systems. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 153: Maintenance
Clean the Max-Light Cartridge Cell Storage of Max-Light Cartridge Cell Correcting Leaks Replace Leak Handling System Parts Replace the Module Firmware Information from Module’s Assemblies This chapter describes the maintenance of the module. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 154: Introduction To Maintenance
Eye damage may result from directly viewing the UV-light produced by the lamp of the optical system used in this product. Always turn the lamp of the optical system off before removing it.  Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 155 If you connect external equipment to the instrument, make sure that you  only use accessory units tested and approved according to the safety standards appropriate for the type of external equipment. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 156: Overview Of Maintenance
A wavelength calibration test should be cell. performed after replacement. Leak sensor drying If leak has occurred. Check for leaks. Leak handling System If broken or corroded. Check for leaks. replacement Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 157: Cleaning The Module
The figures shown in this procedure exemplarily show the Infinity II Multisampler NOT E module. The principle of how to remove and/or install doors works in the same way for all Infinity II modules. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 158 Press the release buttons and pull the front door out. For the Installation of the front door. Insert the hinges into their guides and move the door in until the release buttons click into their final position. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 159: Replace The Deuterium Lamp
If the detector has been in use, the lamp may be hot. If so, wait for lamp to cool down.  The lamp house cover includes a magnet. NOT E Open the doors. Locate the lamp cover. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 160 Do not touch the glass bulb with your fingers. It may reduce the light output. Fix the lamp screws (1.) and reconnect the lamp con- Place the lamp cable in the lamp cover. nector (2.). Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 161 Slide the lamp cover into the top position of the metal 10 Close the doors. front (1.) and press the lamp cover completely in until it clicks (2.). 11 Perform a Wavelength Re-calibration after lamp warm-up. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 162: Replace The Max-Light Cartridge Cell
In case the flow cell is not used for some time (stored), then flush the flow cell with iso-propanol. Remove the Max-Light Cartridge Cell Open the doors. Disconnect the capillaries from the flow cell cartridge. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 163 Flip the cartridge lever towards the front (down). Pull the cartridge holder completely out. Remove the flow cell cartridge. Install the Max-Light Cartridge Cell Insert the flow cell cartridge. Slide the cartridge holder completely into the detector. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 164 Maintenance Replace the Max-Light Cartridge Cell Lift the cartridge lever up into the final position. Reconnect the capillaries. Close the doors. Perform a Cell Test and a Wavelength Verification Test. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 165: Clean The Max-Light Cartridge Cell
The use of demineralized water may improve the cleaning characteristics. 6 Repeat step 4 on page 165. 7 If tests fail again, the flow cell might be replaced if the chromatographic performace cannot be accepted. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 166: Storage Of Max-Light Cartridge Cell
2 Remove the Max-Light Cartridge Cell from the cartridge holder of the detector. 3 Replace the black hoods, that secure the cell light inlet and outlet. 4 Store the Max-Light Cartridge Cell in plastic case provided with the Max-Light Cartridge Flow Cell. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 167: Correcting Leaks
Open the doors. Use tissue to dry the leak sensor area (1). Observe the capillary connections and the flow cell area (2) for leaks and correct, if required. Close the doors. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 168: Replace Leak Handling System Parts
Locate the Leak Adapter (1) and Tubing (2). Press the Leak Adapter down and remove it together Install the Leak Adapter by pressing it into the Main with the tubing. Cover. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 169 Maintenance Replace Leak Handling System Parts Insert the Tubing (approximately 85 mm required for Close the doors. replacement) between Leak Adapter outlet and Leak Panel. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 170: Replace The Module Firmware
When using the detector in a system, all other modules must have firmware from set 6.50 (latest ver- 1100/1200/1260/ sion) or later (main and resident). Otherwise the communication will not work. 1290 series modules Conversion to / emula- G4212A, G4212B, G7117A G4212B tion Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 171: Information From Module's Assemblies
PSI. Serial Number and Firmware Revision The user interface provides module specific information that is stored in the main board. These are for example the serial number, firmware revision. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 172: Parts And Materials For Maintenance
Parts and Materials for Maintenance Overview of Maintenance Parts Accessory Kit This chapter provides information on parts for maintenance. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 173 Max-Light Cartridge Test Cell  OR 2 G4212-60032 HDR Max-Light Cartridge Cell (3.7 mm, V( ) 0.4 µL)  OR 2 G4212-60038 ULD Max-Light Cartridge Cell (10 mm, V( ) 0.6 µL) Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 174 Tube Connector, 90 degree, ID 6.4 5043-1013 Tubing Clip see item 7 in Figure 51 on page 173 5500-1191 InfinityLab Quick Turn Capillary ST 0.12 mm x 280 mm, long socket Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 175: Identifying Cables
Identifying Cables Cable Overview Analog Cables Remote Cables CAN/LAN Cables RS-232 Cables USB Cables This chapter provides information on cables used with the Agilent 1200 Infinity Series modules. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 176: Cable Overview
Identifying Cables Cable Overview Cable Overview Never use cables other than the ones supplied by Agilent Technologies to ensure NOT E proper functionality and compliance with safety or EMC regulations. Analog cables Description 35900-60750 Agilent 35900A A/D converter 01046-60105 Analog cable (BNC to general purpose, spade lugs)
Page 177 1-1, 2-3, 3-2, 4-6, 5-5, 6-4, 7-8, 8-7, 9-9. 5181-1561 RS-232 cable, 8 m USB cables Description 5188-8050 USB A M-USB Mini B 3 m (PC-Module) 5188-8049 USB A F-USB Mini B M OTG (Module to Flash Drive) Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 178: Analog Cables
The other end depends on the instrument to which connection is being made. Agilent Module to 35900 A/D converters p/n 35900-60750 35900 Pin Agilent Signal Name module Not connected Shield Analog - Center Analog + Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 179 Pin Agilent Signal Name module Shield Shield Analog - Center Center Analog + Agilent Module to General Purpose p/n 01046-60105 Pin Agilent Signal Name module Not connected Black Analog - Analog + Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 180: Remote Cables
NOT USED pink SHUT DOWN blue START PREPARE black 1wire DATA violet DGND grey-pink +5V ERI out red-blue PGND white-green PGND brown-green +24V ERI out white-yellow +24V ERI out yellow-brown Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 181 Pin (ERI) Signal Pin (APG) Active (TTL) Pin (RJ45) Start High Request Stop High Ready High Fraction Trig- High Power on High Shut Down High Start High Prepare High Ground Cable Shield- Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 182 Identifying Cables Remote Cables One end of these cables provides a Agilent Technologies APG (Analytical Products Group) remote connector to be connected to Agilent modules. The other end depends on the instrument to be connected to. Agilent Module to Agilent 35900 A/D Converters...
Page 183: Can/Lan Cables
CAN cable, Agilent module to module, 1 m LAN Cables Description 5023-0203 Cross-over network cable, shielded, 3 m (for point to point connection) 5023-0202 Twisted pair network cable, shielded, 7 m (for point to point connec- tion) Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 184: Cables
To connect a USB Flash Drive use a USB OTG cable with Mini-B plug and A socket. Description 5188-8050 USB A M-USB Mini B 3 m (PC-Module) 5188-8049 USB A F-USB Mini B M OTG (Module to Flash Drive) Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 185: Hardware Information
ERI (Enhanced Remote Interface) USB (Universal Serial Bus) Setting the 6-bit Configuration Switch Special Settings Instrument Layout Early Maintenance Feedback This chapter describes the detector in more detail on hardware and electronics. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 186: Firmware Description
APG/ERI remote, • error handling, • diagnostic functions, • or module specific functions like • internal events such as lamp control, filter movements, • raw data collection and conversion to absorbance. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 187 Main and resident firmware must be from the same set. Main FW update Main System Resident System Resident FW Update Figure 52 Firmware Update Mechanism Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 188 G1314B to G1314C), the original feature set is available again. All this specific information is described in the documentation provided with the firmware update tools. The firmware update tools, firmware and documentation are available from the Agilent web. • http://www.agilent.com/en-us/firmwareDownload?whid=69761 Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 189: Electrical Connections
There are no externally accessible fuses because automatic electronic fuses are implemented in the power supply. Never use cables other than the ones supplied by Agilent Technologies to ensure NOT E proper functionality and compliance with safety or EMC regulations.
Page 190: Rear View Of The Module
DE = Germany • JP = Japan • CN = China year and week of last major manufacturing change, e.g. 820 could be week 20 of 1998 or 2008 SSSSS real serial number Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 191 Alphabetic character A-Z (used by manufacturing) Alpha-numeric code 0-9, A-Z, where each combination unambig- uously denotes a module (there can be more than one code for the same module) 00000 Serial number Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 192: Interfaces
Hardware Information Interfaces Interfaces The Agilent InfinityLab LC Series modules provide the following interfaces: Table 28 Agilent InfinityLab LC Series Interfaces Module RS-232 Analog APG (A) Special (on-board) / ERI (E) Pumps G7104A/C G7110B G7111A/B, G5654A G7112B G7120A, G7132A G7161A/B...
Page 193 RS-232C as interface to a computer • USB (Universal Series Bus) as interface to a computer • REMOTE connector as interface to other Agilent products • Analog output connector(s) for signal output Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 194: Overview Interfaces
Remote (ERI) The ERI (Enhanced Remote Interface) connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features as common shut down, prepare, and so on. It allows easy connection between single instruments or systems to ensure coordinated analysis with simple coupling requirements.
Page 195 (L) Request to start run / timetable. Receiver is any module perform- ing run-time controlled activities. PREPARE (L) Request to prepare for analysis (for example, calibration, detec- tor lamp on). Receiver is any module performing pre-analysis activi- ties. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 196: Eri (Enhanced Remote Interface)
ERI replaces the AGP Remote Interface that is used in the HP 1090/1040/1050/1100 HPLC systems and Agilent 1100/1200/1200 Infinity HPLC modules. All new InfinityLab LC Series products using the FUSION core electronics use ERI. This interface is already used in the Agilent Universal...
Page 197 This serial line can be used to read out an EPROM or write into an EPROM of a connected ERI-device. The firmware can detect the connected type of device automatically and update information in the device (if required). Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 198: Usb (Universal Serial Bus)
Short circuit will be detected through hardware. USB (Universal Serial Bus) USB (Universal Serial Bus) - replaces RS232, supports: • a PC with control software (for example Agilent Lab Advisor) • USB Flash Disk Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 199: Setting The 6-Bit Configuration Switch
For specific LAN modes switches 4-5 must be set as required. • For boot resident/cold start modes switches 1+2 or 6 must be UP. Configuration switch Figure 55 Location of Configuration switch (example shows a G7114A/B VWD) Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 200: Special Settings
In resident mode, it is not operable as a module. It only uses basic functions of the operating system for example, for communication. In this mode the main firmware can be loaded (using update utilities). Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 201 Boot Main System / Keep Data Boot Resident System / Keep Data Boot Main System / Revert to Default Data Boot Resident System / Revert to Default Data Note: The setting '0' (down) is essential. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 202: Instrument Layout
• the metal inner cabinet shields the internal electronics from electromagnetic interference and also helps to reduce or eliminate radio frequency emissions from the instrument itself. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 203: Early Maintenance Feedback
The useful maintenance cycle is dependent on the requirements for use. Therefore, the definition of the maximum limits need to be determined based on the specific operating conditions of the instrument. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 204 EMF counters exceed the new EMF limits, the EMF flag will be displayed, providing a reminder that maintenance needs to be scheduled. This function is only available via Agilent Lab Advisor or Instant Pilot. NOT E Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 205: Lan Configuration
Dynamic Host Configuration Protocol (DHCP) General Information (DHCP) Setup (DHCP) Manual Configuration With Telnet With the Instant Pilot (G4208A) This chapter provides information on connecting the module to the Agilent ChemStation PC. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 206: What You Have To Do First
PC network card using a crossover network cable (point-to-point) or • a hub or switch using a standard LAN cable. LAN interface MAC label Figure 57 Location of LAN interfaces and MAC label Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 207: Tcp/Ip Parameter Configuration
TCP/IP parameters after power-on. The parameters may be derived non-volatile memory or initialized with known default values. The initialization mode is selected by the configuration switch, see Table 32 on page 209. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 208: Configuration Switches
Location of Configuration switch (example shows a G7114A/B VWD) The module is shipped with all switches set to OFF, as shown above. To perform any LAN configuration, SW1 and SW2 must be set to OFF. NOT E Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 209: Initialization Mode Selection
The TCP/IP connection will be established using these parameters. The parameters were configured previously by one of the described methods. Non-Volatile Active Parameter Stored Parameter Figure 59 Using Stored (Principle) Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 210 In the Using Default mode, the parameters stored in the memory of the module NOT E are not cleared automatically. If not changed by the user, they are still available, when switching back to the mode Using Stored. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 211: Dynamic Host Configuration Protocol (Dhcp)
2 It may be necessary to fully qualify the hostname with the DNS suffix, e.g. 0030d3177321.country.company.com. 3 The DHCP server may reject the hostname proposed by the card and assign a name following local naming conventions. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 212 Card or the mainboard of above mentioned modules. Table 34 G1369C LAN Interface Card (configuration switch on the card) SW 4 SW 5 SW 6 SW 7 SW 8 Initialization Mode DHCP Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 213 Advisor, Firmware Update Tool) and use MAC address as host name, e.g. 0030d3177321. The LC system should become visible in the control software (see Note in section “General Information (DHCP)” on page 211). Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 214: Manual Configuration
TELNET Session Non-Volatile Stored Parameter Control Module Figure 63 Manual Configuration (Principle) Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 215: With Telnet
When the connection was established successfully, the module responds with the following: Figure 65 A connection to the module is made 3 Type ? and press enter to see the available commands. Figure 66 Telnet Commands Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 216 Initialization mode is Using Stored active TCP/IP settings TCP/IP status - here ready connected to PC with controller software (e.g. Agilent Figure 67 Telnet - Current settings in "Using Stored" mode ChemStation), here not connected Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 217 If the Initialization Mode Switch is changed now to “Using Stored” mode, the NOT E instrument will take the stored settings when the module is re-booted. In the example above it would be 192.168.254.12. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 218: With The Instant Pilot (G4208A)
Instant Pilot - LAN Configuration (Edit mode) 5 Press the Edit button (only visible if not in Edit mode), perform the required changes and press the Done button. 6 Leave the screen by clicking Exit. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 219: Appendix
In Case of Damage Solvents Safety Symbols Waste Electrical and Electronic Equipment (WEEE) Directive Radio Interference Sound Emission Solvent Information Agilent Technologies on Internet This chapter provides addition information on safety, legal and web. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 220: General Safety Information
Do not use this product in any manner not specified by the manufacturer. The protective features of this product may be impaired if it is used in a manner not specified in the operation instructions. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 221: Before Applying Power
Verify that the voltage range and frequency of your power distribution  matches to the power specification of the individual instrument. Never use cables other than the ones supplied by Agilent Technologies  to ensure proper functionality and compliance with safety or EMC regulations.
Page 222: Do Not Operate In An Explosive Atmosphere
Personal injury (for example electrical shock, intoxication) Instruments that appear damaged or defective should be made  inoperative and secured against unintended operation until they can be repaired by qualified service personnel. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 223: Solvents
For details, see the usage guideline for the solvent cabinet. A printed copy of the NOT E guideline has been shipped with the solvent cabinet, electronic copies are available in the Agilent Information Center or via the Internet. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 224: Safety Symbols
A pacemaker could switch into test mode and cause illness. A heart defibril- lator may stop working. If you wear these devices keep at least 55 mm dis- tance to magnets. Warn others who wear these devices from getting too close to magnets. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 225 Do not proceed beyond a caution until you have fully understood and met  the indicated conditions. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 226: Waste Electrical And Electronic Equipment (Weee) Directive
The affixed label indicates that you must not discard this electrical/electronic product in domestic household waste. Do not dispose of in domestic household waste NOT E To return unwanted products, contact your local Agilent office, or see http://www.agilent.com for more information. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 227: Radio Interference
Appendix Radio Interference Radio Interference Cables supplied by Agilent Technologies are screened to provide optimized protection against radio interference. All cables are in compliance with safety or EMC regulations. Test and Measurement If test and measurement equipment is operated with unscreened cables, or used for measurements on open set-ups, the user has to assure that under operating conditions the radio interference limits are still met within the premises.
Page 228: Solvent Information
• mixtures of carbon tetrachloride with 2-propanol or THF. • Avoid the use of dimethyl formamide (DMF). Polyvinylidene fluoride (PVDF), which is used in leak sensors, is not resistant to DMF. Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 229: Agilent Technologies On Internet
Appendix Agilent Technologies on Internet Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at: http://www.agilent.com Agilent InfinityLab LC Series Diode Array Detectors User Manual...
Page 230 • introduction and specifications, • using and optimizing, • troubleshooting and diagnose, • maintenance, • parts identification, • hardware information, • safety and related information. www.agilent.com  Agilent Technologies Inc. 2014-2020 Published in Germany 09/2020 Document No: SD-29000132 Rev. B...

This manual is also suitable for:

Infinity ii 1290 Infinity ii 1260 G7117b G7117a G7117c 1260 infinity ii

Agilent Technologies InfinityLab LC Series User Manual

Table of Contents

1 Introduction

Overview of the Module

Product Description

Optical System

Leak and Waste Handling

Operating Principle

2 Site Requirements and Specifications

3 Using the Module

4 Preparing the Module

5 Optimizing the Detector

Introduction

Optimization Overview

Optimizing for Sensitivity, Selectivity, Linearity and Dispersion

Optimizing Selectivity

Optimizing the Detector Regarding to the System

Warm up of the Detector

6 Troubleshooting and Diagnostics

7 Error Information

What Are Error Messages

General Error Messages

Detector Error Messages

8 Test Functions and Calibration

9 Maintenance

10 Parts and Materials for Maintenance

11 Identifying Cables

12 Hardware Information

13 LAN Configuration

14 Appendix

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Chapters

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Summary of Contents for Agilent Technologies InfinityLab LC Series